Determination of parameters for tobacco mosaic virus cryogenic treatment and freeze-drying

Donev, Todor; Yordanova, A.; Stoimenova, E.; Damjanova, Sonja

doi:10.1007/bf00180405

Cited by 5 publications

(2 citation statements)

References 6 publications

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“…However, significant particle breakage occurred at higher pressures, or if the sample had thawed before water sublimation, suggesting that conditions during freeze-drying critically influence virus integrity. A systematic optimization of freeze-drying conditions for tobamoviruses identified specific temperature and pressure profiles as well as cryoprotectants that sustained up to 89% infectivity [301]. This work significantly advanced the understanding of freeze-drying as a viable method for the long-term storage of tobamoviruses, offering insights into the delicate balance required to maintain viral integrity during the lyophilization process.…”

Section: |6|2 Lyophilization and Storage Of Virus Preparationsmentioning

confidence: 92%

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Wendlandt,

Britz,

Kleinow

et al. 2024

Preprint

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This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages, and presents a novel efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses: from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. Best practice and also rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls and 'forgotten' or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of the multivalent soft-matter nanorods.

show abstract

Section: |6|2 Lyophilization and Storage Of Virus Preparationsmentioning

confidence: 92%

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Wendlandt,

Britz,

Kleinow

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…However, significant particle breakage occurred at higher pressures or if the sample had thawed before water sublimation, suggesting that conditions during freeze-drying critically influence virus integrity. A systematic optimization of freeze-drying conditions for tobamoviruses identified specific temperature and pressure profiles as well as cryoprotectants that sustained up to 89% infectivity [ 313 ]. This work significantly advanced the understanding of freeze-drying as a viable method for the long-term storage of tobamoviruses, offering insights into the delicate balance required to maintain viral integrity during the lyophilization process.…”

Section: Techniques For Virion Enrichment and Storage One By Onementioning

confidence: 99%

Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach

Wendlandt,

Britz,

Kleinow

et al. 2024

Viruses

View full text Add to dashboard Cite

This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages and presents a novel, efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses, from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. The best practices and rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls, and ‘forgotten’ or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwhile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of these multivalent soft-matter nanorods.

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Yordanova

Stoimenova

Donev

2000

Biotechnology Letters

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Determination of parameters for tobacco mosaic virus cryogenic treatment and freeze-drying

Cited by 5 publications

References 6 publications

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach

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