Determination of Pamidronate in Bisphosphonate-Enriched Bone Cement by Ion-Pair Hplc and Capillary Electrophoresis

Abstract

The presence of pamidronate during local use of bisphosphonates (BP)-enriched bone cement was determined. The question was whether pamidronate implanted into the bone cement is eluted. The study was performed on 10 probes of BP-enriched bone cement located in 0.9% NaCl. The probes were incubated for 3 and 6 weeks. Ion-pair HPLC was used for the detection of pamidronate. Then, capillary electrophoresis was applied for quantitative analysis of pamidronate in the 3rd and 6th week after incubation. The presence of pamidronate, eluted from BP-enriched bone cement into 0.9% NaCl solution 3 and 6 weeks after incubation, was demonstrated. These results may explain the changes in the level of cytokine RANKL and bone turnover marker osteoprotegrin in rats’ serum treated with BP-enriched bone cement 3 and 6 weeks after surgery. The possibility of effective local use of BP-enriched bone cement in veterinary medicine was underlined. The results, and the former conducted research, point out that the clinical applications of BP-enriched bone cement in vivo may have some validity in the future.

The presence of pamidronate in bone cement affects serum biochemical markers in the rat

“…Our further results obtained by Ion Pair HPLC and CE methods showed that pamidronate is eluted from the BPenriched cement after three and six weeks of incubation in 0.9% NaCl solution (20). These results may explain the changes in the level of cytokines in rats' serum.…”