Determination of oxymatrine and its metabolite matrine in rat blood and dermal microdialysates by high throughput liquid chromatography/tandem mass spectrometry

Zheng, Hong; Chen, Guangchun; Shi, Lei; Lou, Ziyang; Fangjian, Chen; Jin-hong, HU

doi:10.1016/j.jpba.2008.11.032

Cited by 31 publications

(16 citation statements)

References 12 publications

(12 reference statements)

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“…In addition, due to its short elimination half life of 2.17 h, it must be repeatedly injected over an extended period. Nevertheless, the dermal metabolism of oxymatrine via topical delivery seems to be low compared to its systemic metabolism (Zheng et al, 2009). To our knowledge, few studies about topical and transdermal delivery of oxymatrine have been reported.…”

Section: Introductionmentioning

confidence: 94%

A combination of a microemulsion and a phospholipid complex for topical delivery of oxymatrine

Cao

Ouyang

Wang³

et al. 2011

Arch. Pharm. Res.

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Oxymatrine (OMT), a water-soluble drug, has a very low oral bioavailability because of its low membrane permeability and its biotransformation in the gastrointestinal tract. Formulated as an oxymatrine-phospholipid complex (OMT-PLC) can improve the lipid solubility and effectiveness of OMT. The purpose of this study was to explore the utility of the combination of a microemulsion and an OMT-PLC as a topical delivery vehicle for enhancing the absorption and efficacy of OMT. The solubility of OMT-PLC was determined and phase diagrams of microemulsions were constructed. Various microemulsion formulations were developed and characterized by their physicochemical properties, and their in vitro and in vivo permeability through skin. An optimal microemulsion (ME4), which presented as spherical droplets and consisted of 10.0% OMT-PLC, 8.0% isopropyl myristate, 30.0% Cremophor RH40/polyethylene glycol 400 (1:1) and 52.0% water, was selected. It possessed an average droplet size of 32.4 nm, a low viscosity of 113.7 mPa · s, and a high cloud point of 88°C. Compared to the control solution, ME4 provided better skin permeability in vitro and a higher retention ratio of OMT in skin in vivo. Moreover, ME4 significantly enhanced the antiproliferative activity of OMT on scar fibroblasts. These results indicate that the combination of a microemulsion and a phospholipid complex represents an effective vehicle for topical delivery of OMT.

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Section: Introductionmentioning

confidence: 94%

A combination of a microemulsion and a phospholipid complex for topical delivery of oxymatrine

Cao

Ouyang

Wang³

et al. 2011

Arch. Pharm. Res.

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show abstract

“…Zheng et al [102] conducted the microdialysis combined with HPLC-MS/MS to study the oxymatrine gels in the rats skin and plasma microdialysate samples the topical metabolic parameters, the Oxymatrine in vivo blood vessels and skin probe recoveries were (31.1 ± 7.6)%, and (26.7 ± 0.85)%; the matrine in vivo blood vessels and skin probe recoveries were (39.6 ± 4.7)% and (41.1 ± 1.7)%. The study was the first to clarify the topical oxymatrine after the percutaneous metabolism, the oxymatrine and its metabolite matrine dynamic metabolic process in the skin and blood.…”

Section: Study the Traditional Chinese Medicine Metabolic Percutaneousmentioning

confidence: 99%

Crosstalk the Microdialysis in Scientific Research: from Principle to its Applications

Lee¹,

Di²

2013

Pharm Anal Acta

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“…The metabolism of oxymatrine and its metabolite matrine was investigated using LC -MS/MS [103] . Another report uses microdialysis sampling and LC -MS to probe the extracellular environment of tumors in mice bearing human melanoma xenografts.…”

Section: Off -Line Analysis Using Liquid Chromatographymentioning

confidence: 99%

Analytical Considerations for Microdialysis Sampling

Nandi

Kuhnline

Lunte

2011

Applications of Microdialysis in Pharmaceutical Science

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Determination of oxymatrine and its metabolite matrine in rat blood and dermal microdialysates by high throughput liquid chromatography/tandem mass spectrometry

Cited by 31 publications

References 12 publications

A combination of a microemulsion and a phospholipid complex for topical delivery of oxymatrine

A combination of a microemulsion and a phospholipid complex for topical delivery of oxymatrine

Crosstalk the Microdialysis in Scientific Research: from Principle to its Applications

Analytical Considerations for Microdialysis Sampling

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