Determination of oxygen uptake by tissue grown in an all glass differential microrespirometer

Grunbaum, Benjamin W.; Siegel, Benjamin V.; Schulz, Arthur R.; Kirk, Paul L.

doi:10.1007/bf01224009

Cited by 35 publications

(10 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Routine rates of oxygen consumption were measured with glass micro-respirometers (Grunbaum et al, 1955). The number of larvae used in each determination varied according to larval size, with more individuals being required for the younger stages.…”

Section: Methodsmentioning

confidence: 99%

Physiological Studies on Cancer irroratus Larvae. III. Effects of Temperature and Salinity on the Partitioning of Energy Resources During Development

Johns¹

1982

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Balanced energy budgets were estimated for larval stages of the rock crab Cancer irroratus cultured in 6 combinations of temperature and salinity. The energy consumed by larvae during developn~ent increased in all culture conditions although consumption in 10 'C -30% S was significantly lower than for larvae in other conditions (15 "C -25 ' %o S, 15 "C -30 %O S, 15 "C -35 %o, 24 "C -30%0 S). Maintenance costs for the entire developmental period were highest for larvae cultured at 10°C -30% S and 15°C -25% S. Larvae under these conditions had net growth efficiencies of only 32-34 %. In the other temperature and salinity conditions tested, the amount of energy expended for maintenance was generally equal to the amount of energy converted to new tissue with net growth efficiencies varying between 4 8 and 51 %. These data suggest that the larva's ability to acquire energy resources and effectively partition the energy obtained between maintenance and growth depends on environmental factors. Under certain conditions (lO°C -30% S, 15OC -25 % S ) larvae are unable to compensate physiologically for environmental changes. One consequence of incomplete physiological compensation is a reduced potential for growth and an overall reduction in the organism's ecological fitness within the zooplankton community.

show abstract

Section: Methodsmentioning

confidence: 99%

Physiological Studies on Cancer irroratus Larvae. III. Effects of Temperature and Salinity on the Partitioning of Energy Resources During Development

Johns¹

1982

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…Larval respiration rates were estimated in order to predict rates of weight loss in the absence of food. A single measurement was made in 1980 using all-glass microrespirometry (Grunbaum et al 1955), with 25 freshly collected larvae swimming in 0.5 ml of Florida…”

Section: Larval Respiration Ratesmentioning

confidence: 99%

“…A single measurement was made in 1980 using all-glass microrespirometry (Grunbaum et al 1955), with 25 freshly collected larvae swimming in 0.5 ml of Florida Current seawater at 25°C. Readings were taken every 30 min for 3 h. Larvae were then preserved in 10% formalin buffered with sodium borate and later weighed in a single group as described above for juveniles.…”

Section: Larval Respiration Ratesmentioning

confidence: 99%

Influence of delayed metamorphosis on postsettlement survival and growth in the sipunculan Apionsoma misakianum

Pechenik

Rice

2001

Invertebrate Biology

View full text Add to dashboard Cite

Abstract. Certain stresses experienced by marine larvae from many groups can dramatically reduce aspects of juvenile performance. This study reports the effects of delayed metamorphosis and nutritional stress on survival and growth of the deposit-feeding sipunculan Apionsoma (= Golfingid) misakianum. Approximately 600 larvae collected from the Florida Current plankton were distributed among 3 treatment groups. Ninety larvae (controls) were offered sediment and adult-conditioned seawater 4 d after collection, to induce metamorphosis; larvae of this species could not be induced to metamorphose by increasing the K + concentration of seawater. The remaining 500 larvae were kept swimming for either 2 or 4 weeks, with or without phytoplankton (clone T-ISO). At the end of the periods of prolonged larval swimming, subsampled larvae (360) were induced to metamorphose as in the controls. Surviving individuals were retrieved 6 weeks after the addition of excess sediment in all treatments, and weighed to document growth. Neither delayed metamorphosis nor starvation influenced juvenile survival. However, starving larvae for 2 weeks significantly reduced mean juvenile growth rates relative to the mean growth rate of control individuals (p<0.0001), while prolonging larval life by 4 weeks significantly reduced mean juvenile growth rates (p<0.05) whether or not larvae were fed. Reduced juvenile growth rates may have been caused by nutritional stress experienced by larvae in both the starved and fed treatments. The rapid response of freshly collected larvae to sediment indicates that competent larvae of this species routinely delay metamorphosis in the field. The extent to which they also experience food limitation is not yet clear. If competent larvae are food limited while delaying n letarn jrphosis in the field, our results suggest that juveniles will grow more slowly and may thus exhibit reduced fitness.

show abstract

“…We used a modified all-glass differential micro-respirometer (Greunbaum, Siegel, Schultz, and Kirk, 1955) in which the capillary bores were modified to 0.35 mm diameter and the respirometry vessels were enlarged to a 10 rn1 capacity. This instrument combines simplicity with small size, and makes possible semi-continuous series of measurements over a short period of time.…”

Section: Oxygen Consumption Measurementsmentioning

confidence: 99%

Limits of Nematoscelis megalops in the northwestern Atlantic in relation to Gulf Stream cold core rings. II, Physiological and biochemical effects of expatriation

Boyd¹,

Wiebe²,

Cox³

1979

View full text Add to dashboard Cite

Nematoscelis megalops, a cold water euphausiid commonly found in Northwestern Atlantic Slope Water, is frequently transported in the cores of Gulf Stream cyclonic rings into the Sargasso Sea. The inner core made of cold Slope Water gradually assumes physical and biological characteristics of the surrounding Sargasso Sea. These changes gradually lead to a localized extinction of this species in the core of the ring. Samples of N. megalops taken from the same ring at 6 and 9 months after its formation show a weakened physiological and biochemical condition.Deterioration of ring individuals is evidenced by an increase in body water content and a reduction in total body lipid, carbon, respiration rates, and nitrogen relative to Slope Water individuals. By 6 months it appears that ring N. megalops must su:p,Plement food intake by metabolizing some of their body protein and by 9 months they appear to use lipids as well.A shipboard starvation experiment involving 40 Slope Water individuals showed that physiological and biochemical states similar to those found in individuals from the 9 months old ring could be duplicated in 4 days of complete starvation.

show abstract

Determination of oxygen uptake by tissue grown in an all glass differential microrespirometer

Cited by 35 publications

References 5 publications

Physiological Studies on Cancer irroratus Larvae. III. Effects of Temperature and Salinity on the Partitioning of Energy Resources During Development

Physiological Studies on Cancer irroratus Larvae. III. Effects of Temperature and Salinity on the Partitioning of Energy Resources During Development

Influence of delayed metamorphosis on postsettlement survival and growth in the sipunculan Apionsoma misakianum

Limits of Nematoscelis megalops in the northwestern Atlantic in relation to Gulf Stream cold core rings. II, Physiological and biochemical effects of expatriation

Contact Info

Product

Resources

About