Determination of oxycodone and its major metabolites in haematic and urinary matrices: Comparison of traditional and miniaturised sampling approaches

Protti, Michele; Catapano, Maria Carmen; Dekel, Boaz Gedaliahu Samolsky; Rudge, James; Gerra, Gilberto; Lorenzo, Somaini; Mandrioli, Roberto; Mercolini, Laura

doi:10.1016/j.jpba.2018.01.043

Cited by 54 publications

(29 citation statements)

References 25 publications

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“…The RRE and ME were consistent throughout the calibration line, and the validation could therefore be performed with an LLOQ of albendazole of 5 ng/ml. Nevertheless, the partially obtained ME and poor RRE of DBS samples is a disadvantage in comparison to the Mitra device, as has been already described in earlier studies (16)(17)(18).…”

Section: Resultsmentioning

confidence: 83%

Pharmacokinetics of Albendazole, Albendazole Sulfoxide, and Albendazole Sulfone Determined from Plasma, Blood, Dried-Blood Spots, and Mitra Samples of Hookworm-Infected Adolescents

Schulz

Neodo

Coulibaly

et al. 2019

Antimicrob Agents Chemother

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Albendazole is an effective anthelmintic intensively used for decades. However, profound pharmacokinetic (PK) characterization is missing in children, the population mostly affected by helminth infections. Blood microsampling would facilitate PK studies in pediatric populations but has not been applied to quantify albendazole's disposition. Quantification methods were developed and validated using liquid chromatography-tandem mass spectrometry to analyze albendazole and its metabolites albendazole sulfoxide and albendazole sulfone in wet samples (plasma and blood) and blood microsamples (dried-blood spots [DBS]; Mitra). The use of DBS was limited by a matrix effect and poor recovery, but the extraction efficiency was constant throughout the concentration range. Hookworm-infected adolescents were venous and capillary blood sampled posttreatment with 400 mg albendazole and 25 mg/kg oxantel pamoate. Similar half-life (t 1/2 ϭ ϳ1.5 h), time to reach the maximum concentration (t max ϭ ϳ2 h), and maximum concentration (C max ϭ 12.5 to 26.5 ng/ml) of albendazole were observed in the four matrices. The metabolites reached C max after ϳ4 h with a t 1/2 of ca. 7 to 8 h. A statistically significant difference in albendazole sulfone's t 1/2 as determined by using DBS and wet samples was detected. C max of albendazole sulfoxide (288 to 380 ng/ml) did not differ among the matrices, but higher C max of albendazole sulfone were obtained in the two microsampling devices (22 ng/ml) versus the wet matrices (14 ng/ml). In conclusion, timeconcentration profiles and PK results of the four matrices were similar, and the direct comparison of the two microsampling devices indicates that Mitra extraction was more robust during validation and can be recommended for future albendazole PK studies.

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Section: Resultsmentioning

confidence: 83%

Pharmacokinetics of Albendazole, Albendazole Sulfoxide, and Albendazole Sulfone Determined from Plasma, Blood, Dried-Blood Spots, and Mitra Samples of Hookworm-Infected Adolescents

Schulz

Neodo

Coulibaly

et al. 2019

Antimicrob Agents Chemother

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“…The tip is capable of absorbing a fixed liquid sample volume (10, 20, or 30 µL), according to its size and independently of sample density or viscosity. After absorption, the specimen is left to dry, and is then ready for storage, shipping or immediate extraction, and analysis [13,14]. The use of fixed-volume dried microsamples offers several advantages over traditional, large-volume biosamples.…”

Section: Introductionmentioning

confidence: 99%

Blood and Plasma Volumetric Absorptive Microsampling (VAMS) Coupled to LC-MS/MS for the Forensic Assessment of Cocaine Consumption

2020

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Reliable, feasible analytical methods are needed for forensic and anti-doping testing of cocaine and its most important metabolites, benzoylecgonine, ecgonine methyl ester, and cocaethylene (the active metabolite formed in the presence of ethanol). An innovative workflow is presented here, using minute amounts of dried blood or plasma obtained by volumetric absorptive microsampling (VAMS), followed by miniaturized pretreatment by dispersive pipette extraction (DPX) and LC-MS/MS analysis. After sampling 20 µL of blood or plasma with a VAMS device, the sample was dried, extracted, and loaded onto a DPX tip. The DPX pretreatment lasted less than one minute and after elution with methanol the sample was directly injected into the LC-MS/MS system. The chromatographic analysis was carried out on a C8 column, using a mobile phase containing aqueous formic acid and acetonitrile. Good extraction yield (> 85%), precision (relative standard deviation, RSD < 6.0%) and matrix effect (< 12%) values were obtained. Analyte stability was outstanding (recovery > 85% after 2 months at room temperature). The method was successfully applied to real blood and plasma VAMS, with results in very good agreement with those of fluid samples. The method seems suitable for the monitoring of concomitant cocaine and ethanol use by means of plasma or blood VAMS testing.

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“…Furthermore, no storage-induced degradation occurred over a period of 1 year when the DBS samples were stored at room temperature with desiccant and no light exposure. [24][25][26][27] and thus, it appears that DBS can accurately be used for quantitative purposes. In this respect, it should be noted that the amount of blood was non-volumetrically applied to the filter card and the entire spots were used for analysis in the present study.…”

Section: Discussionmentioning

confidence: 99%

Single‐dose administration of clenbuterol is detectable in dried blood spots

Solheim

Jessen

Mørkeberg³

et al. 2020

Drug Testing and Analysis

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Clenbuterol is a β2‐agonist prescribed for asthmatic patients in some countries. Based on its anabolic and lipolytic effects observed in studies on rodents and in livestock destined for food production, clenbuterol is abused by bodybuilders and athletes seeking leanness. Urinary clenbuterol analysis is part of routine doping analysis. However, the collection of urine samples is time‐consuming and can be intimidating for athletes. Dried blood spot (DBS) appears attractive as an alternative matrix, but the detectability of clenbuterol in humans through DBS has not been investigated. This study evaluated if clenbuterol could be detected in DBS and urine collected from six healthy men after oral intake of 80 μg clenbuterol. The DBS and urine samples were collected at 0, 3, 8, 24, and 72 h post‐ingestion, with additional urine collections on days 7 and 10. Using LC–MS/MS, it was shown that clenbuterol could be detected in all DBS samples for 24 h post‐ingestion and with 50% sensitivity 3 days after ingestion. The DBS method was 100% specific. Evaluation of analyte stability showed that clenbuterol is stable in DBS for at least 365 days at room temperature when using desiccant and avoiding light exposure. In urine, clenbuterol was detectable for at least 7–10 days after ingestion. Urinary clenbuterol concentrations below 5 ng/mL were present in some subjects 24 h after administration. Collectively, these data indicate that DBS are suitable for routine doping control analysis of clenbuterol with a detection window of at least 3 days after oral administration of 80 μg.

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Determination of oxycodone and its major metabolites in haematic and urinary matrices: Comparison of traditional and miniaturised sampling approaches

Cited by 54 publications

References 25 publications

Pharmacokinetics of Albendazole, Albendazole Sulfoxide, and Albendazole Sulfone Determined from Plasma, Blood, Dried-Blood Spots, and Mitra Samples of Hookworm-Infected Adolescents

Pharmacokinetics of Albendazole, Albendazole Sulfoxide, and Albendazole Sulfone Determined from Plasma, Blood, Dried-Blood Spots, and Mitra Samples of Hookworm-Infected Adolescents

Blood and Plasma Volumetric Absorptive Microsampling (VAMS) Coupled to LC-MS/MS for the Forensic Assessment of Cocaine Consumption

Single‐dose administration of clenbuterol is detectable in dried blood spots

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