Determination of Oxfendazole in Cow Milk by Reversed-Phase High-Performance Liquid Chromatography

Tsina, Irene; Matin, S.B.

doi:10.1002/jps.2600700805

Cited by 9 publications

(4 citation statements)

References 7 publications

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“…2 Different analytical techniques were applied for quantitative estimation of oxfendazole including potentiometric titration, 3,4 radio immunoassay, 5 spectrophotometric methods 6 and HPLC methods even alone or in presence of other compounds. [7][8][9][10][11][12][13][14][15][16][17] Oxfendazole is an amide group containing compound that made it highly sensitive to hydrolytic degradation in basic conditions with the production of the degradation product 2-amino-5-Phenylsulfinyl benzimidazole. There is no stability indicating analytical methods were reported for determination of oxfendazole in presence of its degradation product.…”

Section: Introductionmentioning

confidence: 99%

RP- HPLC and TLC- densitometric methods for determination of oxfendazole in the presence of its alkali -induced degradation product

Salama¹,

Attia²,

Mohamad³

et al. 2018

JAPLR

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Accurate, selective, and sensitive reversed phase high performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC)-densitometry methods have been developed and validated for determination of oxfendazole in the presence of its alkaliinduced degradation product. The developed RP-HPLC method was based on the separation of the two components using 0.05M KH 2 PO 4 (adjusted to pH=5), methanol and actonitrile (50:30:20, by volume) as a mobile phase in isocratic elution mode on BDS Hypersil C 18 column at a flow rate of 1mL min −1 and ultraviolet (UV) detection at 225nm. The components were well resolved from each other with significantly different R t values of 7.3 and 4.05min for oxfendazole and its alkali-induced degradation product, respectively. The method was applied in the range of (3-15µg mL-1). On the other hand, TLC-densitometric method depends on the separation and quantitation of oxfendazole and its alkali-induced degradation product on TLC silica gel 60 F254 plates, using chloroformmethanol and glacial acetic acid (90:8:2, by volume) as the developing system followed by densitometric measurement at 292nm. The studied components were well resolved from each other with significantly different R f values of 0.75 and 0.2 for oxfendazole and its degradation product, respectively. The method was applied in the range of (0.5-6µg / spot). The developed methods were validated according to the International Conference on Harmonization (ICH) guidelines demonstrating good accuracy and precision. The results were statistically compared with those obtained by the reported method, and no significant difference was found.

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Section: Introductionmentioning

confidence: 99%

RP- HPLC and TLC- densitometric methods for determination of oxfendazole in the presence of its alkali -induced degradation product

Salama¹,

Attia²,

Mohamad³

et al. 2018

JAPLR

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“…Chromatographic [21][22][23][24][25][26][27] and radioimmunoassay 28 were devoted by different researchers for assaying OFA in biological materials like serum, milk, muscle and plasma. OFA was assayed before by ratiospectra derivative spectrophotometric, 29 chemometric calibration 30 and the continuous wavelet transform 31 techniques in pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

Potentiometric determination of piroxicam and oxfendazole in pharmaceuticals

Rajendraprasad¹,

Basavaiah²

2016

10.5267/j.ccl

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Piroxicam (POC) and oxfendazole (OFA) are two widely used veterinary non-steroidal antiinflammatory and anthelmintic medicines, respectively. Simple, precise and accurate and costeffective methods for the determination of POC and OFZ in bulk drug and in its dosage forms have been developed and validated. The methods are based on the potentiometric titration of compound with acetous perchloric acid in glacial acetic acid medium using modified glasssaturated calomel electrode system. The methods are applicable over the ranges 1.5-15 mg and 7.5-15 mg, for POC and OFA, respectively. The proposed methods were successfully applied to the determination of active substances in their pharmaceutical dosage forms. A statistical comparison was made on the results of proposed methods with those obtained for reference method. The intra-day and inter-day precision and accuracy values obtained were satisfactory with RSD and RE values less than 3%. Excipients in tablets did not interfere to the assay as shown by the recovery study via standard addition technique with mean percentage recoveries in the range 97.8-100.6%.

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“…Different analytical techniques were applied for quantitative estimation of oxfendazole including potentiometric titration 3,4 , radio immunoassay 5 , colorimetric (based on the measurement of green colored manganate obtained due to oxidation of oxfendazole by permanganate in an alkaline medium at 610 nm) and direct measurement (at 290 nm in the acetic acid-water (1:1) solvent system) spectrophotometric methods 6 and HPLC methods either alone or in presence of other compounds [7][8][9][10][11][12][13][14][15][16][17] .…”

Section: Figure 1 Chemical Structure Of Oxfendazolementioning

confidence: 99%

Development and Validation of Spectrophotometric Methods for Quantitative Estimation of Oxfendazole in Presence of Its Alkali-induced Degradation Product: A Comparative Study

Salama

Attia

Mohamad

et al. 2017

Journal of Advanced Pharmacy Research

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Objectives: This study aimed to develop and validate three simple, accurate, selective, reproducible and sensitive spectrophotometric methods for the determination of oxfendazole in the presence of its alkali-induced degradation product without preliminary separation. Methods: (A) first derivative spectrophotometry ( 1 D) method at 298.9 nm, (B) first derivative of ratio spectra ( 1 DD) method at 295.4 nm and (C) area under the curve (AUC) method at wavelength ranges of (290-294 nm) and (297-301 nm). All methods were applied in the range of (1-10µg mL -1 ). Results: These methods were validated and successfully applied for determination of oxfendazole in Unifendazole® suspension. The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision. Conclusion: The proposed methods are simple, rapid, economic, accurate and precise to determine oxfendazole in the presence of its alkali-induced degradation product without previous separation steps.

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Determination of Oxfendazole in Cow Milk by Reversed-Phase High-Performance Liquid Chromatography

Cited by 9 publications

References 7 publications

RP- HPLC and TLC- densitometric methods for determination of oxfendazole in the presence of its alkali -induced degradation product

RP- HPLC and TLC- densitometric methods for determination of oxfendazole in the presence of its alkali -induced degradation product

Potentiometric determination of piroxicam and oxfendazole in pharmaceuticals

Development and Validation of Spectrophotometric Methods for Quantitative Estimation of Oxfendazole in Presence of Its Alkali-induced Degradation Product: A Comparative Study

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