Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour

Wang, Junliang; Xia, Qing; Zhang, Anping; Hu, Xiaoyan; Lin, Chunmian

doi:10.1631/jzus.b11a0180

Cited by 31 publications

(25 citation statements)

References 15 publications

(14 reference statements)

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“…The biosensor had a LOD of 0.19 and 1.51 mM respectively for methyl parathion and malathion [1], as shown in Table 1. Wang et al [8], reported that the inhibition method using purified esterase from wheat flour could detect different pesticides in samples of lettuce. The LOD were between 0.17 and 1.70 mg/L.…”

Section: Other Inhibition-based Pesticide Biosensorsmentioning

confidence: 99%

See 1 more Smart Citation

Enzyme-Based Biosensors for Electrochemical Detection of Pesticides–A Mini Review

Soropogui

Jameel

Salim

2018

IJEEI

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Despite their important contribution in increasing crops production, most pesticides are harmful to humans and living beings and can persist in the environment over long a long duration. Traditional chromatographic methods of analysis are expensive and cumbersome. Biosensor technology appears therefore as an efficient and economical alternative for fast detection of pesticides. The devices are portable, rapid, and highly sensitive. Other important features of the devices are their relatively high sensistivity and low response time. Enzymatic biosensors for pesticide detection rely either on the inhibition mechanism or on the catalytic activity of the immobilized enzyme toward a specific pesticide. Metal and carbon based nanomaterials are being widely used as immobilization support owing to novel characteristics such as biocompatibility and enhanced electron transfer ability for sensitive electrochemical detection, among others. This review focusses on the electrochemical detection of organophosphorus pesticides, delineating the limit of detection and response time of biosensors toward a wide range of organophosphorus pesticides.

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Section: Other Inhibition-based Pesticide Biosensorsmentioning

confidence: 99%

“…Other types of enzymes used are alkaline and acid phosphatases, organophosphorus hydrolase, and tyrosinase [7]. Immobilization of plant esterases is a relatively new trend in enzyme-based biosensor design for simple and convenient detection of pesticides [1], [8], [9].…”

Section: Introductionmentioning

confidence: 99%

Enzyme-Based Biosensors for Electrochemical Detection of Pesticides–A Mini Review

Soropogui

Jameel

Salim

2018

IJEEI

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“…Akwanga is one out of the 13 local Government Area in Nasarawa State, Nigeria and the major occupation here is farming with a total population of 113,430 [20]. It has a land area of 996 km 2…”

Section: The Study Areamentioning

confidence: 99%

“…The term is used often to describe virtually all organic phosphorus containing compounds especially when dealing with neurotoxin compounds [1]. These insecticides are one of the most common classes of chemicals used for the control of insects on vegetables because of their high efficacy and broad spectrum of activity [2]. Organophosphorus residues are likely to occur in vegetables such as lettuce and cabbage.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Organophosphorous Pesticide Residues in Pumpkin, Spinach and Sorrel Leaves Grown in Akwanga, Nasarawa State, Nigeria

Ibrahim¹,

Yakubu²,

Nnamonu³

et al. 2018

JEP

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Three green leafy vegetable samples of pumpkin leaves, spinach leaves, and sorrel leaves were collected from three different locations in a farm in Akwanga and were tested for the presence of organophosphorus (OP) compounds. The concentrations of all the pesticide residues in the vegetable samples were determined using gas chromatography with mass spectrometry (GC-MS). The organophosphorus pesticides detected include carbaryl (0.052 mg/kg) in pumpkin, this is below European maximum residues limit (EU MRL) of 0.1 mg/kg, Dimethoate was found in pumpkin (0.165 mg/kg), spinach (0.103 mg/kg) and sorrel (0.250 mg/kg) all above the EU MRL of 0.05 mg/kg. Dichlofenthion was detected in pumpkin (0.308 mg/kg), pirimiphos methyl was detected in pumpkin and spinach (0.428 mg/kg and 0.149 mg/kg respectively), all these were below the EU MRL of 0.5 mg/kg. Chlorpyrifos was found in spinach (0.230 mg/kg) and sorrel (0.192 mg/kg) only spinach was above the EU MRL of 0.2 mg/kg. The pesticide residue Bromophosethyl was detected in all vegetables, pumpkin leaves (0.501 mg/kg), sorrel (1.571 mg/kg) and spinach (7.981 mg/kg) all above the EU MRL of 0.5 mg/kg. The remaining pesticides detected were all below their EU MRL value; these are Ethion found in spinach (0.167 mg/kg), Methyl Parathion in spinach (0.103 mg/kg) and sorrel (0.335 mg/kg). The levels of some of the organophosphorus pesticide residues found in vegetables were above the maximum residue limits (MRLs) set by the European Union. This calls for laws to regulate the use and circulation of such chemicals. Based on the observation made in these studies,

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“…Preparation of quartz fiber coating with ZnO/g-C 3 N 4 The APTES functionalized fiber was vertically immersed into a serum bottle through a pinhead, which was filled with a mixture of Zn(NO 3 ) 2 $6H 2 O (0.05 M) and hexamethylenetetramine (CH 2 ) 6 N 4 (0.05 M) in solution necessary for ZnO hybridized with g-C 3 N 4 preparation, and heated at 90 C in oil bath pan for 4 h. In high temperature, the ZnO/g-C 3 N 4 composite was synthesized from g-C 3 N 4 and Zn 2þ through salt formation, thermal decomposition and crystallization on g-C 3 N 4 due to the eNH 2 /eNHe/]Ne as nucleation site. Thus ZnO nanoflower are tightly growed on the g-C3N4 nanosheet.…”

Section: Preparation Of G-c 3 N 4 Nanosheetmentioning

confidence: 99%

In situ hydrothermal growth of ZnO/g-C3N4 nanoflowers coated solid-phase microextraction fibers coupled with GC-MS for determination of pesticides residues

Zhang

Gao

Huang

et al. 2016

Analytica Chimica Acta

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Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour

Cited by 31 publications

References 15 publications

Enzyme-Based Biosensors for Electrochemical Detection of Pesticides–A Mini Review

Enzyme-Based Biosensors for Electrochemical Detection of Pesticides–A Mini Review

Prevalence of Organophosphorous Pesticide Residues in Pumpkin, Spinach and Sorrel Leaves Grown in Akwanga, Nasarawa State, Nigeria

In situ hydrothermal growth of ZnO/g-C3N4 nanoflowers coated solid-phase microextraction fibers coupled with GC-MS for determination of pesticides residues

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