Determination of opiates and cocaine in urine by high pH mobile phase reversed phase UPLC–MS/MS

Berg, Thomas; Lundanes, Elsa; Christophersen, Asbjørg S.; Strand, Dag Helge

doi:10.1016/j.jchromb.2008.12.052

Cited by 121 publications

(64 citation statements)

References 32 publications

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“…higher retention and improved selectivity) and MS sensitivity (i.e. better signal-to-noise because of the improved mobile phase desolvatation in presence of a high percentage of organic modifier) [19,30]. Therefore, a pH value of 9 was selected as a good compromise between sufficient retention/selectivity and elevated MS sensitivity.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

“…In the field of doping analysis, UHPLC-MS-MS strategy was employed for determining between 30 and 130 prohibited substances in urine in a single analytical run [16][17][18], using a simple ''dilute and shoot'' as sample preparation. Finally, Berg et al [19] developed and validated an SPE-UHPLC-MS-MS method allowing the determination of six opiates, cocaine and benzoylecgonine in less than 6 min, reequilibration included. They also mentioned that their method has been routinely used in more than 2,000 urine samples with two replicates of each sample.…”

Section: Introductionmentioning

confidence: 99%

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High-Throughput Screening of Drugs of Abuse in Urine by Supported Liquid–Liquid Extraction and UHPLC Coupled to Tandem MS

Maquille

Guillarme

Rudaz

et al. 2009

Chroma

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A qualitative method, involving supported liquid-liquid extraction (SLE) and ultra high pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS-MS), was developed for the rapid tentative identification of various drugs of abuse in urine. In this study, 28 drugs and metabolites were covered by the screening procedure. Before analysis, urine samples were extracted by SLE and good extraction recoveries were obtained for most investigated compounds. The UHPLC strategy was then selected for the rapid separation of amphetamines, cocaine, opiates and related compounds in urine. Using columns packed with sub-2 lm particles, analysis time was reduced down to 2 min, while maintaining acceptable performance. Finally, the detection was by tandem MS operating in the single reaction monitoring (SRM) mode. The most intense transition was selected for the different drugs and SRM dwell times set at 5 ms, to maintain sufficient data points across the narrow UHPLC peaks. The tentative identification of the drugs of interest, including amphetamines, opiates and cocaine, was based on both, retention times and mass spectrometry information. With the proposed method, limits of detection were estimated at about 1 ng mL -1 and the applicability was assessed by successfully analyzing several samples of drug abusers. Finally, this study demonstrates the potential of UHPLC coupled to tandem MS for the rapid screening of drugs of abuse in urine.

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Section: Chromatographic Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High-Throughput Screening of Drugs of Abuse in Urine by Supported Liquid–Liquid Extraction and UHPLC Coupled to Tandem MS

Maquille

Guillarme

Rudaz

et al. 2009

Chroma

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“…These include: spectrophotometry 5,6 , immuno chromatography 7 , potentiometry 8,9 , simultaneous voltammetric and amperometric 10 , gc-mass 1,11,26 , cyclicvoltametry and amperometry 12 , cyclicvoltametry 13,14 , sequential injection analysis 4 ,chemiluminescence 15 , kinetic potentiometric 9 , high performance liquid chromatography [16][17][18][19] , gas chromatography 20 , capillary electrophoresis 21 , ion mobility spectrometry 22,23 . These methods are efficient, but require expensive instrument and are expensive and many of them have needing time to complete the determination.…”

Section: Oriental Journal Of Chemistrymentioning

confidence: 99%

Kinetic Spectrophotometric Determination of Morphine in Pharmaceutical Samples

Keyvanfard¹,

Amri²

2014

Orient J Chem

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A new, sensitive, simple, inexpensive and fast kinetic spectrophotometric method was developed for the determination of trace amounts of morphine over the range of 12-60ng/mL. The method is based on the catalytic effect of morphine on the reaction of bromate and methylene bluein acidic media is reported. The reaction was monitored spectrophotometrically by 60 ng/ml measuring the decrease in absorbance of methylene blue at 665 nm with a fixed-time 0.5-2.5 min from initiation of the reaction. The detection limit is 0.8 ng/mL and relative standard deviation of 12and 52ng/mLmorphine for 6 replicate measurements was 1.50 and 0.87%respectively. The method 12 and 52 ng/ml was applied to the determination of morphine inpharmaceutical samples % respectively.

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“…The confirmation and quantification analyses for positive findings from screening were carried out using alternative chromatographic systems (11)(12)(13). Heroin exposure was assessed by the verification in both blood and urine of 6-MAM and/or a morphine to codeine concentration ratio greater than unity (M/C > 1), as suggested previously by us (14) and others (15).…”

Section: Sampling and Analyses Of Blood And Urinementioning

confidence: 99%

Surveillance of abused drugs in forensic autopsy cases in Norway

Konstantinova-Larsen¹,

Normann²,

Arnestad³

et al. 2011

Nor J Epidemiol

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High drug related mortality has been registered in Norway. Although comparison between countries relies on a standard international coding system of diseases, different practices in verifying cause of death and applying codes could lead to variation. The comparison gives no information on drug findings or drug patterns underlying the cause of deaths. To evaluate deaths involving illicit drugs in Norway, we examined drug patterns in 2735 forensically examined post-mortem samples collected from 15-64 year-old individuals from 2000 to 2009. There were four times as many men as women among the deceased illicit drug users, and the majority were in the age group 25-44 years. The number of deceased showing signs of illicit drug use has gradually declined during the study period. The decline was found among younger individuals, while a larger proportion of the deceased were above 45 years of age in 2009, compared to 2000. Cases positive for heroin, ethanol, ecstasy and flunitrazepam were fewer in 2009, while the prevalence of amphetamine, cannabis, methadone and other opioids has increased. The prevalence of methamphetamine has increased ten fold, and the prevalence of benzodiazepines doubled. Thus, the drug pattern and age of the deceased has changed markedly during the last 10 years. Heroin and ethanol use has partly been substituted by use of amphetamines, cannabis, benzodiazepines and other opioids. This change could possibly be explained by the prolonged survival of drug users on substitution treatment and by the reduced toxicity of consumed drugs.

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Determination of opiates and cocaine in urine by high pH mobile phase reversed phase UPLC–MS/MS

Cited by 121 publications

References 32 publications

High-Throughput Screening of Drugs of Abuse in Urine by Supported Liquid–Liquid Extraction and UHPLC Coupled to Tandem MS

High-Throughput Screening of Drugs of Abuse in Urine by Supported Liquid–Liquid Extraction and UHPLC Coupled to Tandem MS

Kinetic Spectrophotometric Determination of Morphine in Pharmaceutical Samples

Surveillance of abused drugs in forensic autopsy cases in Norway

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