Determination of Omeprazole, Esomeprazole and Pantoprazole by Quenching the fluorescence of Eosin Y

Abstract

A non-extractive, simple and sensitive spectrofluorimetric method with good selectivity has been described for the determination of the proton pump inhibitors namely; Omeprazole, Esomeprazole magnesium and Pantoprazole sodium that are used for the treatment of peptic ulcer disease. The method is based on the quenching the fluorescence intensity of the eosin Y dye, as the result of the ion-pair complex formation of the studied drugs with the dye in the presence of acetate buffer solution of pH 3.5. The quenching of the eosin Y fluorescence intensity was measured spectrofluorimetrically at 540 nm after excitation at 352 nm. At the optimum reaction conditions, the quenching values of fluorescence (∆F) and concentrations were rectilinear over the concentration ranges of 0.5–13.0, 0.7-15 and 0.3-15 µg/mL for Omeprazole, Esomeprazole magnesium and Pantoprazole sodium respectively. The recovery % values were in the range 99.92-100.56% and relative standard deviation values range was0.430-2.521 for all the studied drugs. The method was applied successfully for determination of above drugs in their pharmaceutical formulations as capsule, tablet and injectables. The method was free from interferences of common excipients.

Plant extract mediated synthesis of ZnO and CeO2 nanoparticles for spectrofluorometric assay of omeprazole and domperidone in pharmaceuticals

“… Analytical techniques Reagent Linear range LOD Ref. Spectrophotometry OMZ iodine and DDQ 0.1–3.0 0.5–25.0 μg ml −1 0.03 0.14 μg ml −1 [ 62 ] DOM, methyl cellulose 10–50 μg ml −1 4.59 μg ml −1 [ 63 ] Fluorescence OMZ, Tb 3+ -1,10-phenanthroline 0.05–10 μg ml −1 0.016 μg ml −1 [ 64 ] DOM, methanol 50 v/v. 0.1–3.0 μg mL −1 5.77 × 10 −3 μg mL −1 [ 65 ] Electrochemical OMZ, Pencil graphite electrode 30-200 μmol L −1 7.52 μmol L −1 [ 66 ] DOM Conductive Glassy Carbon electrode 0.1–100 Μ mol L −1 12.0 mol L −1 [ 67 ] Chromatography OMZ, RP-HPLC (80:20) water, Methanol mobile phase 6–20 μg ml −1 1.425 × 10 −5 μg mL −1 [ 68 ] DOM, RP-HPLC method, 70:30 methanol:phosphate buffer at a flow rate of 1 mL/min was used as mobile phase 15-150 μg ml −1 0.0372 μg ml −1 [ 69 ] Prop...…”