Determination of Ofloxacin in Pharmaceuticals, Human Urine and Serum Using a Potentiometric Sensor

Abstract

An ofloxacin-selective electrode was developed for application in pharmaceutical preparations, serum and urine analysis. The electrode is based on tetrakis[3,5-bis(trifluoromethyl)phenyl]borate as ion exchanger in a poly(vinyl chloride) membrane. The sensor displayed a Nernstian response for ofloxacin over a wide concentration range (3 10) with a slope of 55 AE 1 mV per decade of concentration and a practical detection limit of 1 10 À6 mol L À1. The electrode was successfully applied to the determination of ofloxacin in samples with no pretreatment steps, adequate accuracy and precision and recoveries within the intervals of 94-107 %.