Determination of Oenothein B as the Active 5-α-Reductase-Inhibiting Principle of the Folk Medicine Epilobium parviflorum

Lesuisse, Dominique; Berjonneau, J.; Ciot, C.; Devaux, PF; Doucet, B.; Gourvest, Jean-François; Khemis, B.; Lang, Calvin A.; LeGrand, Roy D.; Lowinski, Maryse; Maquin, Pedro; Parent, Angèle; Schoot, Bernard; Teutsch, G.; Chodounská, H.; Kasal, Alexander

doi:10.1021/np960231c

Cited by 45 publications

(34 citation statements)

References 22 publications

(30 reference statements)

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“…Flow cytometry analysis indicated that the antiproliferative effect could be ascribed to an inhibition of the progression of the cell cycle, in agreement with our previous results obtained with the extracts in toto [19]. The antiproliferative action of the nonpolar fraction may be ascribed to flavonoids, macrocyclic ellagitannins, or sterols identified in these extracts [20,21]. In a previous study [14], flavonoids and sitosterol derivates were detected in the ethyl acetate phase (nonpolar fraction).…”

Section: Discussionsupporting

confidence: 90%

“…This may thus explain the greater potency of E. rosmarinifolium in inhibiting cell proliferation and, at the same time, also suggests that oenothein B may play a relevant role in such an antiproliferative effect. Oenothein B is a macrocyclic ellagitanin, a dimer of the tellimagrandin I unit, that can inhibit the activity of 5·-reductase and aromatase [20,24]. Oenothein B has been shown to have antitumor effects in vivo in mice [22] and to inhibit cellular growth in vitro principally because of decreased cell viability [23,39].…”

Section: Discussionmentioning

confidence: 99%

“…Information on the active component/s of Epilobium extracts is limited; however, sterols (ß-sitosterol), ellagitannins (oenothein B), flavonoids (quercetin, myricetin, kaempferol), and a few other compounds were identified [20,21]. Among these, the macrocyclic ellagitanin oenothein B has been shown to have in vivo and in vitro antitumor activities [22,23] and to inhibit two enzymes involved in BPH development, 5·-reductase and aromatase [20,24].…”

Section: Introductionmentioning

confidence: 99%

“…Among these, the macrocyclic ellagitanin oenothein B has been shown to have in vivo and in vitro antitumor activities [22,23] and to inhibit two enzymes involved in BPH development, 5·-reductase and aromatase [20,24].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Effect of Epilobium Extracts on Human Cell Proliferation

Vitalone¹,

McColl²,

Thome³

et al. 2003

Pharmacology

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We have previously shown that extracts of different Epilobium species, a phytotherapeutic agent used in folk medicine as a treatment for benign prostatic hyperplasia, inhibit proliferation of human prostate cells. The selectivity of this effect was evaluated in four different human cell lines (PZ-HPV-7, normal prostate cells; LNCaP, transformed prostate cells; HMEC, mammary cells, and 1321N1, astrocytoma cells). Different extracts of Epilobium species (E. rosmarinifolium, E. spicatum, and E. tetragonum) had similar growth-inhibitory effects in all cell lines tested, indicating a lack of specificity for prostate cells. Inhibition of DNA synthesis was mostly due to the nonpolar fraction of the extracts which is expected to contain flavonoids and sterols. Polar fractions were devoid of activity with the exception of that from E. rosmarinifolium. This species is the most potent in the antiproliferative effect and contains the highest concentration of oenothein B, a hydrolyzable ellagitannin. Oenothein B inhibited DNA synthesis in all four cell lines tested. Extracts of E. angustifolium (the Linné denomination of E. spicatum) and of E. spicatum from different sources were compared for their ability to inhibit DNA synthesis and for their oenothein B content. The E. angustifolium extract contained an amount of oenothein B 40-fold higher than the other extract of the same species and was ten times more potent in inhibiting DNA synthesis in a human prostate cell line. These results indicate that Epilobium extracts inhibit proliferation of prostate cells in a nonspecific manner. Oenothein B may play a role in this effect, but other active compounds are also present. The difference observed between extracts from the same species underscores the importance of determination and standardization of active ingredients in phytotherapeutic agents.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of the Effect of Epilobium Extracts on Human Cell Proliferation

Vitalone¹,

McColl²,

Thome³

et al. 2003

Pharmacology

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“…In past, Epilobium species have used as a preventative measure for benign prostatic hyperplasia (BPH); [41][42][43][44] and many reports suggest of plant possessing anti-proliferative, anti-inflammatory and antioxidant activities. [45][46][47][48][49][50][51][52] More specifically, Epilobium extracts have shown noticeable effects on activities of several key enzymes including 5α-reductase and aromatase, 53,54 caspase 3 55 and metaloproteinases.…”

Section: Canadian Willow Herbmentioning

confidence: 99%

Addressing Androgenetic Alopecia-A Complex Disorder-with a Multilateral Treatment Strategy

Patel¹

2017

MOJBB

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Antioxidant and antiinflammatory effect of Epilobium parviflorum Schreb.

Hevesi

Houghton

Habtemariam

et al. 2008

Phytotherapy Research

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Epilobium parviflorum Schreb. (Onagraceae) is used for the treatment of benign prostatic hyperplasia (BPH), but its biological action is not entirely identified. This paper aims to report data on E. parviflorum with respect to its antioxidant and antiinflammatory effects. The aqueous acetone extract of E. parviflorum showed higher antioxidant effect in the DPPH assay than well known antioxidants and inhibited the lipid peroxidation determined by the TBA assay (IC(50) = 2.37 +/- 0.12 mg/mL). In concentrations of 0.2-15.0 microg/mL the extract possessed a protective effect, comparable to catalase (250 IU/mL), against oxidative damage, generated in fibroblast cells. In the COX inhibition assay E. parviflorum decreased the PGE(2) release, so showing inhibition of the COX-enzyme (IC(50) = 1.4 +/- 0.1 microg/mL).

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Determination of Oenothein B as the Active 5-α-Reductase-Inhibiting Principle of the Folk Medicine Epilobium parviflorum

Cited by 45 publications

References 22 publications

Characterization of the Effect of <i>Epilobium</i> Extracts on Human Cell Proliferation

Characterization of the Effect of <i>Epilobium</i> Extracts on Human Cell Proliferation

Addressing Androgenetic Alopecia-A Complex Disorder-with a Multilateral Treatment Strategy

Antioxidant and antiinflammatory effect of Epilobium parviflorum Schreb.

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