Determination of octreotide and assessment of matrix effects in human plasma using ultra high performance liquid chromatography–tandem mass spectrometry

Ismaiel, Omnia A.; Zhang, Tianyi; Jenkins, Rand; Karnes, H. Thomas

doi:10.1016/j.jchromb.2011.05.039

Cited by 41 publications

(19 citation statements)

References 17 publications

(43 reference statements)

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“…A 15-fold dilution was sufficient to reduce matrix affects in samples of tomato, orange and leek juice for the quantification of 53 different pesticides (Ferrer et al, 2011). Column-switching and pre-column back-flushing is an effective method of removing PLs from the samples during analysis by determining exactly when the PLs will appear and switching to an alternative column during that time period (Ismaiel et al, 2011). PLs tend to adhere to analytical columns, especially hydrophobic columns, and can then elute later in the analysis (Kushon and Pike, 2013;Silvester and Smith, 2012), so back-flushing is used to clear the column and prevent contamination of later samples.…”

Section: Techniques For Minimizing Matrix Effects Owing To Pls Duringmentioning

confidence: 99%

“…PLs tend to adhere to analytical columns, especially hydrophobic columns, and can then elute later in the analysis (Kushon and Pike, 2013;Silvester and Smith, 2012), so back-flushing is used to clear the column and prevent contamination of later samples. This can both extend column life and improve the quality of the analysis (Ismaiel et al, 2011). Many different solvents have been tested to determine the best choice for clearing PLs that have adhered to the column, including acetonitrile (ACN), methanol (MeOH), dichloromethane (DCM), isopropanol (IPA), chloroform, n-hexane, and various mixtures of these, some including water.…”

Section: Techniques For Minimizing Matrix Effects Owing To Pls Duringmentioning

confidence: 99%

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The impact of phospholipids and phospholipid removal on bioanalytical method performance

Carmical

Brown

2016

Biomedical Chromatography

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Phospholipids (PLs) are a component of cellmembranes, biological fluids and tissues. These compounds are problematic for the bioanalytical chemist, especially when PLs are not the analytes of interest. PL interference with bioanalysis highly impacts reverse-phase chromatographic methods coupled with mass spectrometric detection. Phospholipids are strongly retained on hydrophobic columns, and can cause significant ionization suppression in the mass spectrometer, as they outcompete analyte molecules for ionization. Strategies for improving analyte detection in the presence of PLs are reviewed, including in-analysis modifications and sample preparation strategies. Removal of interfering PLs prior to analysis seems to be most effective atmoderating thematrix effects fromthese endogenous cellular components, and has the potential to simplify chromatography and improve column lifetime. Products targeted at PL removal for sample pre-treatment, as well as products that combine multiplemodes of sample preparation (i.e. Hybrid SPE), show significant promise inmediating the effect on PL interference in bioanalysis.

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Section: Techniques For Minimizing Matrix Effects Owing To Pls Duringmentioning

confidence: 99%

Section: Techniques For Minimizing Matrix Effects Owing To Pls Duringmentioning

confidence: 99%

The impact of phospholipids and phospholipid removal on bioanalytical method performance

Carmical

Brown

2016

Biomedical Chromatography

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“…This combination has been recently used by several authors to determine different types of compounds [8][9][10][11][12][13][14]. Compared to the conventional offline SPE and LC separation systems, these improvements are considerably valuable for analysing emerging compounds in complex environmental samples.…”

Section: Introductionmentioning

confidence: 99%

On-line solid-phase extraction coupled to ultra-performance liquid chromatography with tandem mass spectrometry detection for the determination of benzotriazole UV stabilizers in coastal marine and wastewater samples

2012

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Benzotriazoles are a group of UV absorbing compounds considered emerging contaminants that are used in different personal care products, and therefore, it is of high interest to develop sensitive and fast methods for investigating their presence in the environment. In this work, we present the development and application of a novel method based on on-line solid-phase extraction coupled to ultra-performance liquid chromatography with tandem mass spectrometry detection (SPE-UPLC-MS/MS) for the determination of seven benzotriazole UV stabilizers (BUVSs) in coastal marine and wastewater samples. This process is compared with a conventional off-line SPE procedure followed by UPLC-MS/MS. The parameters affecting the performance of the sample preparation and determination processes were evaluated. The results indicate that the on-line procedure provides for better sensitivity and reproducibility and is faster and easier than the off-line procedure. The detection limits and quantification limits achieved were in the range of 0.6-4.1 ng∙L(-1) and 2.1-14 ng∙L(-1) and relative standard deviation between 6.2 and 10%. The developed method was applied to coastal marine and wastewater samples from Gran Canaria Island (Spain). All of the BUVSs studied were detected in the samples from wastewater treatment plants and two were found in the seawater samples (UV P in the range of 2.8-4.4 ng∙L(-1) and UV 360 between 3.6 and 5.2 ng∙L(-1)).

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“…An optimal way of performing ''true'' global profiling would be to introduce the samples straight into a high-resolution mass spectrometer, without any pretreatment, to detect all metabolites that can be ionized. This is currently not possible due to mainly two reasons: the current mass spectrometers are unable to analyze the entire span of chemically diverse metabolites and; ion suppression/enhancement effects caused by co-occurring substances will distort the MS signal (Calbiani et al 2006;Ismaiel et al 2011). To solve these issues most researchers utilize sample preparation combined with chromatographic separation to decrease the complexity of the sample and remove substances that have a negative impact on the MS detection.…”

Section: Sampling and Sample Preparationmentioning

confidence: 99%

“…However, a wide metabolite coverage is more likely to describe perturbation(s) of a biological system in regards to the study objective, thus relevant for the generation of a hypothesis. Nonetheless, some research groups use specific sample preparation solutions based on solid phase extraction (SPE) technology to selectively remove phospholipids, one major reason for ion suppression during plasma sample analysis (Calbiani et al 2006;Ismaiel et al 2011). It has been demonstrated that these SPE methods efficiently remove most phospholipids in plasma samples (Lahaie et al 2010;Tulipani et al 2013) and therefore, in most cases, improve metabolite coverage by reduced ion suppression/enhancement.…”

Section: Sampling and Sample Preparationmentioning

confidence: 99%

LC–MS based global metabolite profiling: the necessity of high data quality

et al. 2016

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LC-MS based global metabolite profiling currently lacks detailed guidelines to demonstrate that the obtained data is of high enough analytical quality. Insufficient data quality may result in the failure to generate a hypothesis, or in the worst case, a false or skewed hypothesis. After assessing the literature, it is apparent that an analytically focused summary and critical discussion related to this subject would be beneficial for both beginners and experts engaged in this field. A particular focus will be placed on data quality, which we here define as the degree to which a set of parameters fulfills predetermined criteria, similar to the established guidelines for targeted analysis. However, several of these parameters are difficult to assess since holistic approaches measure thousands of metabolites in parallel and seldom include predefined knowledge of which metabolites will differ between sample groups. In this review, the following parameters will be discussed in detail: reproducibility, selectivity, certainty of metabolite identification and metabolite coverage. The review systematically describes the generic workflow for LC-MS based global metabolite profiling and highlights how each separate part may affect data quality. The last part of the review describes how data quality can be evaluated as well as identifies areas where additional improvement is needed. In this review, we provide our own analytical opinions in regards to evaluation and, to some extent, improvement of data quality.

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Determination of octreotide and assessment of matrix effects in human plasma using ultra high performance liquid chromatography–tandem mass spectrometry

Cited by 41 publications

References 17 publications

The impact of phospholipids and phospholipid removal on bioanalytical method performance

The impact of phospholipids and phospholipid removal on bioanalytical method performance

On-line solid-phase extraction coupled to ultra-performance liquid chromatography with tandem mass spectrometry detection for the determination of benzotriazole UV stabilizers in coastal marine and wastewater samples

LC–MS based global metabolite profiling: the necessity of high data quality

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