Determination of Ochratoxin A in Italian Red Wines by Molecularly Imprinted Solid Phase Extraction and HPLC Analysis

Abstract

An extraction method based on molecularly imprinted polymer prepared through a mimic template approach was used for the determination of ochratoxin A in 17 red wines from different geographical regions of Italy. Sample loading (wine sample diluted 1:1 with 1% v/v aqueous solution of PEG 8000), washing (2 mL water/acetonitrile 4:1 v/v), and elution (2 mL of acetonitrile/acetic acid 98:2 v/v) conditions allowed the optimization of the extraction method, capable of preconcentrating ochratoxin A below the maximum permitted level of 2 ng/mL. Under optimized conditions, recoveries of ochratoxin A from spiked samples ranged from 88 to 102% with sample volumes up to 20 mL. The HPLC determination by fluorescence detection allowed limits of detection and quantification, respectively, of 0.075 and 0.225 ng/mL. Sample extractions by an immunoaffinity protocol showed the method to be comparable, demonstrating the potential of the imprinting approach to substitute for the current immunoaffinity method.

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

“…However, the reported LOD and LOQ values (0.025 and 0.08 μg/L, respectively) are about 1.5-fold higher than those obtained in this work. LOD (0.075 μg/L) and LOQ (0.225 μg/L) values reported in a similar study that used another synthesized MIP to determine OTA in Italian red wines were 4.4-fold higher than those of the BSA-based SPE method (Giovannoli, Passini, Di Nardo, Anfossi, & Baggiani, 2014). Additionally, Rhouati, Paniel, Meraihi, and Marty (2011) developed and tested an oligosorbent (aptamer) for the analytical determination of OTA in beer, reporting a LOD of 0.2 μg/L, which is also far over the response obtained herein using albumin as sorbent (0.017 μg L −1 ).…”

A novel molecularly imprinted method with computational simulation for the affinity isolation and knockout of baicalein from Scutellaria baicalensis

“…Thus, the obtained MIPs are able to specifically rebind with the templates in a strong and selective way. Owing to their mechanical and chemical stability, low cost and high selectivity, MIPs have been exploited for catalysis, sensors, drug delivery, solid‐phase extraction (SPE) and chromatographic separation (Wang et al ., ; Resmini, ; Yang and Zhao, ; Bereli et al ., ; Li et al ., , ; Giovannoli et al ., ). Molecularly imprinted solid‐phase extraction (MISPE) is one of the most successful and useful applications for the separation and enrichment of many analytes from a complex matrix (Zeng et al ., ; Scorrano et al ., ; Sadeghi and Jahani, ; Guan et al ., ).…”

Comprehensive study on the degradation of ochratoxin A in water by spectroscopic techniques and DFT calculations

“…1881/2006. 11 Immunochemical methods, [12][13][14][15] liquid chromatography coupled to uorescence spectroscopy 14,[16][17][18][19][20] and mass spectrometry 15, 20,21 are commonly used for OTA determination. OTA, i.e.…”