Determination of ochratoxin A in roasted coffee and corn by immunoaffinity column cleanup with high-performance thin layer chromatography

Chompurat, S.; Sato, Kazuyoshi; Mahakarnchanakul, Warapa; Sirison, Jiraporn; Kladpan, S.

doi:10.2520/myco1975.2006.suppl4_197

Cited by 2 publications

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“…There are some reports on contamination of OTA and infection of OTA-producing fungi in green coffee in Vietnam 14), 15) and Thailand 13) . Only two reports on OTA in Vietnamese roasted coffee and Thai roasted coffee have been published 16), 17) , but there are no reports in international journals on ochratoxins in Thai instant coffee. The aim of this study was to survey OTA and OTB in commercial coffee from Vietnam and Thailand using an in-house immunoaffinity-column (IAC) HPLC method 6) .…”

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Occurrence of ochratoxin A and ochratoxin B in commercial coffee in Vietnam and Thailand

Wongworapat

Soontornjanagit

et al. 2016

Proc. Jpn. Assoc. Mycotoxicol.

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Occurrence of ochratoxin A (OTA) and ochratoxin B (OTB) in 32 Vietnamese roasted coffees, 30 Thai roasted coffees and 38 Thai instant coffees was performed using an immunoaffinity column-HPLC method. Twenty-six of Vietnamese roasted coffees were contaminated with overall average 0.75 μg/kg of OTA and 11 samples were contaminated with overall average 0.20 μg/kg of OTB. Four of Thai roasted coffees were contaminated with overall average 0.17 μg/kg of OTA and one sample was contaminated with 0.56 μg/ kg of OTB. Twenty-eight of Thai instant coffees were contaminated with overall average 2.19 μg/kg of OTA, and OTB from these samples was not detected. There was no sample more than OTA regulatory limits of European Union. Therefore, it seems that the risk of ochratoxins in retail coffees in Vietnam and Thailand was acceptably low. This report is the first on occurrence of OTB in Vietnamese roasted coffee and ochratoxins in Thai instant coffee.

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“…OTB was detected in only one sample (0.56 μg/kg). Chompurat et al reported that 3 (6.8%) of 44 Thai roasted coffee (Arabica-Robusta blended) were contaminated with OTA with a positive average of 2.9 μg/kg in the range <1-4.6 μg/kg 17) . The positive average of OTA in the report was 4.3-times higher than that in this study.…”

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confidence: 99%

Occurrence of ochratoxin A and ochratoxin B in commercial coffee in Vietnam and Thailand

Wongworapat

Soontornjanagit

et al. 2016

Proc. Jpn. Assoc. Mycotoxicol.

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“…IM-TLC was used to detect OTA with an LOD of 0.5 mg/kg (Santos and Vargas, 2002 ). In another study, Immunoaffinity column high-performance thin layer chromatography (IM-HPTLC) has also been used to detect OTA with limits of detection <0.28 μg/kg (Chompurat et al, 2007 ). More recently, an LOD of around 0.4 ng/mL was reported with multitime-regenerated IM (Liu X. et al, 2018 ).…”

Section: Ochratoxin a Detectionmentioning

confidence: 99%

Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

Nao

Wang

et al. 2020

Front. Chem.

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Ochratoxin A (OTA) is a mycotoxin that is widespread throughout the world. It contaminates foods such as vegetables, fruits, and rice. It harms human health and has potential carcinogenic effects. The G-quadruplex (G4) is a tetraplexed DNA structure generated from guanine-rich DNA that has found emerging use in aptamer-based sensing systems. This review outlines the status of OTA contamination and conventional detection methods for OTA. Various G4-based methods to detect OTA developed in recent years are summarized along with their advantages and disadvantages compared to existing approaches.

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Determination of ochratoxin A in roasted coffee and corn by immunoaffinity column cleanup with high-performance thin layer chromatography

Cited by 2 publications

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Occurrence of ochratoxin A and ochratoxin B in commercial coffee in Vietnam and Thailand

Occurrence of ochratoxin A and ochratoxin B in commercial coffee in Vietnam and Thailand

Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

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