Determination of Ochratoxin A in Beer by Immunoaffinity Cleanup and Liquid Chromatography with Tandem Mass Spectrometry

Omote, M.; Kitagawa, Yasushi; Mochizuki, Naoki

doi:10.1094/asbcj-2008-0226-02

Cited by 6 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since cereals are raw materials of beer and beer-based drinks, it is necessary to consider that the risk of multi-contamination exists in beer and beer-based drinks. Therefore, various analytical methods have been developed for each group of mycotoxin in beer, [11][12][13][14][15][16][17][18][19] but each requires a different clean-up method (e.g., immunoaffinity column, solid phase extraction cartridge, and multi-functional cartridge) and specific analytical conditions for liquid chromatography (LC) with different detectors (e.g., ultraviolet detector, fluorescence detector, and mass spectrometer). As a result, analyses of the regulated mycotoxins become time-consuming, and quality control of foods related to mycotoxins become difficult to practice.…”

Section: Introductionmentioning

confidence: 99%

Development of a Multi-mycotoxin Analysis in Beer-based Drinks by a Modified QuEChERS Method and Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

2011

View full text Add to dashboard Cite

An analytical method was developed for the identification and quantification of 15 mycotoxins (patulin, nivalenol, deoxynivalenol, aflatoxin B1, B2, G1, G2, M1, T-2 toxin, HT-2 toxin, zearalenone, fumonisin B1, B2, B3, and ochratoxin A) in beer-based drinks (beer, low-malt beer, new genre, and nonalcoholic) by a modified QuEChERS method and an ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC/MS/MS). Mycotoxins were extracted from samples using acetonitrile with sodium chloride, anhydrous magnesium sulfate, and sodium citrate, and were then purified with a solid phase extraction (SPE) cartridge including C18. The UHPLC conditions were also examined to establish its optimal conditions for separation. Fifteen mycotoxins were separated in a total of 6.5 min, and were quantified in the optimal mobile phase conditions. Determinations performed using this method produced high correlation coefficients of 15 mycotoxins (R > 0.99) and recovery rates ranging from 70.3 to 110.7% with good repeatability (relative standard deviation RSD < 14.6%). Further, 24 commercial beer-based drinks in Japan were analyzed using this method, and nivalenol, deoxynivalenol, and fumonisins were detected in several samples, but always under the limit of quantification (< 5 ng/mL). These results suggest that the health risk to consumers from beer-based drinks in Japan is relatively low.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of a Multi-mycotoxin Analysis in Beer-based Drinks by a Modified QuEChERS Method and Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

2011

View full text Add to dashboard Cite

show abstract

Current literature in mass spectrometry

2008

J. Mass Spectrom.

View full text Add to dashboard Cite

In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Reviews; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (5 Weeks journals ‐ Search completed at 30th. July 2008)

show abstract

Problems on LC-MS/MS Analysis to Ensure Food Safety

Mochizuki

2011

YAKUGAKU ZASSHI

View full text Add to dashboard Cite

An accurate analysis is required to address various issues concerning the food safety. Many risk factors, such as agricultural chemical residues, residual veterinary drugs, mycotoxins, food additives, and carcinogens produced during food processing may be present in foodstuŠs. High-performance liquid chromatograph-tandem mass spectrometry (LC-MS/MS) is an analytical system advanced in terms of sensitivity, selectivity, and ‰exibility. The system has been widely applied to food analysis. Food contains a wide variety of nutritional components, which may cause interference with food analyses. Then, sample preparation to remove such interference and an appropriate choice of an analytical technique is required. Even with LC-MS/MS, the analytical reliability may be reduced by matrix eŠects, due to interference from food components. In this review, we summarize issues on using LC-MS/MS to achieve good analyses for the food safety and discuss how to address it. The topic especially focuses on matrix eŠects.

show abstract

Determination of Ochratoxin A in Beer by Immunoaffinity Cleanup and Liquid Chromatography with Tandem Mass Spectrometry

Cited by 6 publications

References 19 publications

Development of a Multi-mycotoxin Analysis in Beer-based Drinks by a Modified QuEChERS Method and Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

Development of a Multi-mycotoxin Analysis in Beer-based Drinks by a Modified QuEChERS Method and Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

Current literature in mass spectrometry

Problems on LC-MS/MS Analysis to Ensure Food Safety

Contact Info

Product

Resources

About