Determination of Nucleic Acids by a Resonance Light-Scattering Technique with α,β,γ,δ-Tetrakis[4- (trimethylammoniumyl)phenyl]porphine

Abstract

The resonance light-scattering technique, using a spectrofluorometer, was first developed as a sensitive instrumental analysis method. At pH 7.48 and ionic strength 0.004, the extent of light-scattering of alpha, beta, gamma, delta-tetrakis[4-(trimethylammoniumyl)phenyl]porphine (TAPP) is enhanced by nucleic acids near 432 nm. There are linear relationships between the enhanced extents of light-scattering and the concentrations of nucleic acids in the range of 1.8 x 10(-7)-10.8 x 10(-7) M for calf thymus and fish sperm DNA and in the range of 1.8 x 10(-7)-1.8 x 10(-6) M for yeast RNA. The limit of determination (3 sigma) is 4.1 x 10(-8) M for calf thymus DNA, 4.6 x 10(-8) M for fish sperm DNA, and 6.7 x 10(-8) M for yeast RNA. Mechanism study indicates that nucleic acids react with the title porphyrin in two modes, depending on the concentrations of nucleic acids. When the molar ratio of nucleic acids to TAPP is smaller than 4:1, the hypochromicity and fluorescence quenching of TAPP by nucleic acids appear, and the enhancement of resonance light-scattering can be observed. When the molar ratio of nucleic acids to TAPP is larger than 4:1, a new fluorescent complex is formed.

Determination of Proteins with Fast Red VR by a Corrected Resonance Light-Scattering Technique

“…[2][3][4][5][6][7][8] It has been proved that the enhanced RLS signals which resulted from the aggregation and assembly processes can be applied to highly sensitive quantifications of DNA, 9,11 proteins, [12][13][14] ions, 15 surfactants 16 and clinical medicines [17][18][19] by using a common spectrofluorometer. These measurements, however, depend on the measuring conditions, including the molecular absorption, instrument conditions, reflection and scattering of the inside and outside walls of the absorption cell.…”

Determination of DNA with Cetyltrimethylammonium Bromide by the Measurement of Resonance Light Scattering

“…If the instrumental conditions are adjusted unchanged, the enhanced RLS signals are generally related to (1) the concentration and the size of the solute in the aqueous solution; (2) the absorption features of the organic dyes; and (3) the charges of the interacting components. [12][13][14][15] Cetyltrimethylammonium bromide (CTMAB) can be used as the initial precipitant in the extraction of DNA from tissues or cells. 16 The interaction mechanism is the electrostatic attraction between the negative charges of DNA and the positive charges of CTMAB to form ion associates.…”

A label-free method for studying DNA sequence recognition of mitoxantrone based on resonance light-scattering technique

“…The successful application of it in analytical chemistry was firstly reported in 1993, in which Pasternack et al 16,17 successfully studied the J-assembly of trans-bis (N-methylpyridinium-4-y1) diphenylporphine and its copper(II) derivative on DNA using a common fluorescence spectrophotometer. In 1996, Huang et al 18,19 brought RLS technique into the quantitative analytical chemistry and completed the determination of DNA. Subsequently, RLS technique has obtained a wide application in analytical chemistry because of its simplicity, low cost and reliability.…”