Determination of noradrenaline and dopamine in Chinese herbal extracts from Portulaca oleracea L. by high-performance liquid chromatography

Chen, Juan; Shi, Yan‐Ping; Liu, Jingyan

doi:10.1016/s0021-9673(03)00786-6

Cited by 85 publications

(36 citation statements)

References 10 publications

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“…3). Chromatographic data confirmed the results, which have been reported by Chen and Colleagues (Chen et al, 2003). According to the results ( -1 DW) was evaluated (0.927 < desirability < 0.934).…”

Section: Analysis Of Experimental Designsupporting

confidence: 86%

“…The flow rate was 1 mL.min -1 with UV detection wavelength at 280 nm and the column temperature was 25°C. The quantification of the noradrenaline was carried out using an external standard calibration method (Chen et al 2003).…”

Section: Design Of Experimentsmentioning

confidence: 99%

“…Antitumor effects in vivo (Shen et al, 2013) and in vitro (Tian et al, 2013) model and several powerful pharmaceutical activities for example soothing, hepatoprotective, neuropharmacological, antiinflammatory, antidiabetic, bronchodilator, antihypertensive, antioxidant and containing the noradrenaline hormone has been reported (Amirul Alam et al, 2014). Noradrenaline and Dopamine are the major catecholamine bioactive compounds present in P. oleracea L. as an immune system modulator for treatment of shock (Chen et al, 2003). Over the past few years, tissue culture systems for P. oleracea L. has been studied in the callus induction, plant regeneration (Safdari and Kazemitabar 2009;Safdari and Kazemitabar 2010) and Ldopamine; noradrenaline content in hairy root culture (Ahmadi Moghadam et al, 2014;Ahmadi Moghadam et al, 2011;Pirian and Piri 2013;Pirian et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.)

Ghorbani

Omidi

et al. 2015

Turkish JAF Sci.Tech.

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Common purslane (Portulaca oleracea L.) is an annual plant as one of the natural sources for noradrenaline hormone. In this research, hairy root culture of purslane was established by using Agrobacterium rhizogenes strain ATCC 15834. In the following, Box-Behnken model of response surface methodology (RSM) was employed to optimize B5 medium for the growth of P. oleracea L. hairy root line. According to the results, modelling and optimization conditions, including sucrose, CaCl 2 .H 2 O, H 2 PO 4 and NO 3 -/NH 4 + concentrations on maximum dry weight (0.155 g) and noradrenaline content (0.36 mg.g -1 DW) was predicted. These optimal conditions predicted by RSM were confirmed the enhancement of noradrenaline production as an application potential for production by hairy root cultures.

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Section: Analysis Of Experimental Designsupporting

confidence: 86%

Section: Design Of Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.)

Ghorbani

Omidi

et al. 2015

Turkish JAF Sci.Tech.

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“…Based on the good separation of the catecholamines using calix [4]-modified sol-gel column, the potential of establishing a simple extraction-CEC method to apply this column to determinate DA and NE in P. oleracea L. was investigated. The sample solutions were prepared by a method previously described by Chen et al [46]. Briefly, after being air-dried and crushed to powder, 0.5013 g sample was extracted with 25 mL of 0.1 M HCl solution in an ultrasonator for 1.5 h. The extract was then filtered through a filter paper and a 0.45 mm filter membrane for analysis.…”

Section: Analytical Applicationmentioning

confidence: 99%

A novel sol-gel calix[4]arene-modified capillary column for open-tubular capillary electrochromatography

Tian

Zeng

2006

Electrophoresis

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A novel open-tubular CEC (OT-CEC) column was prepared by immobilized 5,11,17,23-tetra-tert-butyl-25,27-diethoxy-26,28-dihydroxy-calix[4]arene (Calix[4]) on fused-silica capillary column with sol-gel technology. Calix[4] was initially reacted with gamma-glycidoxypropyltrimethoxysilane (KH-560) to form a new sol-gel precursor (calix[4]-KH-560), and then mixed with another precursor, namely tetraethoxysilane (TEOS). After hydrolysis and condensation, a sol-gel matrix was obtained, in which calix[4] was incorporated. Successful calix[4]-modified sol-gel coating was suggested by infrared (IR) spectra and greatly decreased EOF. In comparison with OT columns prepared by the sol-gel method with TEOS and KH-560 only, the calix[4]-modified sol-gel column showed greatly improved separation of isomeric toluidines, nitrophenols, picolines and neurotransmitters with structural similarity. Migration time and theoretical plate number reproducibility were satisfactory with RSDs less than 1 and 6% each for within column runs and not more than 3 and 7% each for column-to-column measurements, using toluidine and nitrophenol isomers as test solutes on this column. High separation efficiencies (96,000-300,000 plates/m) for basic toluidine isomers were obtained. This column was also successfully employed to combine extraction procedure for the determination of dopamine (DA) and norepinephrine (NE) in Portulaca oleracea L. The recoveries of DA and NE were 93.3 and 94.2%, respectively.

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“…We have developed several HPLC methods for determining effective constituents in plants or in their pharmaceutical preparations [23][24][25]. This paper describes a simple, rapid, and accurate method using HPLC coupled with photodiode array (PDA) detection for the analysis of the five flavonoids in S. mongolica.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of five flavonoids inSaussurea mongolicaFranch by high-performance liquid chromatography

Chen

Shi

2012

Acta Chromatographica

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Summary.A high-performance liquid chromatographic (HPLC) technique coupled with photodiode array (PDA) detection has been proposed for simultaneous determination of five flavonoids, i.e. quercetin 3-O-β-D-glucopyranoside, quercetin 4′-methoxy-3-O-β-Dgalactopyranoside, kaempferol 3-O-α-L-rhamnopyranoside, asebotin, and kaempferol 7-methxoy-3-O-α-L-rhamnopyranoside in extract of the whole plant of Saussurea mongolica Franch. The optimum conditions for separation were achieved on a 4.6 × 250 mm i.d., 5-μm particle, C 18 column with acetonitrile and 1% acetic acid (20:80, v/v) as the mobile phase at a flow rate of 1.0 mL min −1 . For all the analytes, a good linear regression relationship (r of >0.999) was obtained between peak area and concentration over a relatively wide range. The method was validated for repeatability, precision, stability, and accuracy. Seven different extraction procedures were investigated for preparation of the sample solution. The validated method was successfully applied to simultaneous analysis of these flavonoids in S. mongolica and was found to be simple and efficient.

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Determination of noradrenaline and dopamine in Chinese herbal extracts from Portulaca oleracea L. by high-performance liquid chromatography

Cited by 85 publications

References 10 publications

Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.)

Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.)

A novel sol-gel calix[4]arene-modified capillary column for open-tubular capillary electrochromatography

Simultaneous determination of five flavonoids inSaussurea mongolicaFranch by high-performance liquid chromatography

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