Determination of nomifensine in rat brain by high-performance liquid chromatography with fluorescence detection

D'Aranno, V.; Mancinelli, Angelo; Borsini, Franco; Furio, Martino; Meli, Alberto

doi:10.1016/s0378-4347(00)83398-8

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…A few methods have been reported for the determination of NOM in pharmaceutical preparations and biological fluids. Several methods, such as spectrophotometric and fluorimetric , colorimetric , high‐performance liquid chromatographic (HPLC) , gas chromatogaphic‐mass spectrometric (GC‐MS) and thin layer chromatographic methods have been developed. However, using fluorescamine (FC) as a derivatization probe to determine NOM has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of nomifensine in human plasma and urine by derivatization with fluorescamine

Ulu

2011

Luminescence

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A sensitive, simple and rapid spectrofluorimetric method was developed for the determination of nomifensine in human plasma and urine. The present method was based on the derivatization by fluorescamine in phosphate buffer at pH 4.0 to produce a highly fluorescent product which was measured at 488 nm (excitation at 339 nm). The method was validated according to the ICH guidelines with respect to linearity, limit of detection, limit of quantification, accuracy, precision, recovery and robustness. The assay was linear over the concentration ranges 100-2,000 and 50-2,000 ng/mL for plasma and urine, respectively. The limits of detection were calculated to be 13.9 and 7.5 ng/mL for plasma and urine, respectively. The method was successfully applied to the analysis of the drug in human plasma and urine.

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Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of nomifensine in human plasma and urine by derivatization with fluorescamine

Ulu

2011

Luminescence

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Resolution of Nomifensine Enantiomers on Selected Achiral and Chiral Stationary Phases Using High Performance Liquid Chromatography

Hefnawy¹,

Stewart²

1999

Analytical Letters

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Determination of nomifensine in rat brain by high-performance liquid chromatography with fluorescence detection

Cited by 2 publications

References 15 publications

Spectrofluorimetric determination of nomifensine in human plasma and urine by derivatization with fluorescamine

Spectrofluorimetric determination of nomifensine in human plasma and urine by derivatization with fluorescamine

Resolution of Nomifensine Enantiomers on Selected Achiral and Chiral Stationary Phases Using High Performance Liquid Chromatography

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