Determination of nitrotyrosine and tyrosine by high-performance liquid chromatography with tandem mass spectrometry and immunohistochemical analysis in livers of mice administered acetaminophen

Ishii, Yuji; Iijima, Masayoshi; Umemura, Takashi; Nishikawa, Akiyoshi; Iwasaki, Yusuke; Ito, Rie; Saito, Koichi; Hirose, Masao; Nakazawa, Hiroyuki

doi:10.1016/j.jpba.2006.02.045

Cited by 80 publications

(35 citation statements)

References 25 publications

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“…While the most recent study reported relative increase in 3-NT brain content in Alzheimer's disease [101] , there are no reports of quantitative brain levels of this oxidative stress marker in humans. The 3-NT in mice liver is reported at concentration ranging from 0.17 to 0.3 pmol per mg protein [60] . Thus, our report contains novel data on brain 3-NT levels and the evidence of an increase in brain levels of 3-NT in autism.…”

Section: Implications Of Increased Cerebellar 3-nt In Autismmentioning

confidence: 99%

Oxidative Stress in Autism: Elevated Cerebellar 3-nitrotyrosine Levels

Sajdel-Sulkowska¹,

Lipiński²,

Windom³

et al. 2008

American J. of Biochemistry and Biotechnology

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It has been suggested that oxidative stress and/or mercury compounds play an important role in the pathophysiology of autism. This study compared for the first time the cerebellar levels of the oxidative stress marker 3-nitrotyrosine (3-NT), mercury (Hg) and the antioxidant selenium (Se) levels between control and autistic subjects. Tissue homogenates were prepared in the presence of protease inhibitors from the frozen cerebellar tissue of control (n=10; mean age, 15.5 years; mean PMI, 15.5 hours) and autistic (n=9; mean age 12.1 years; mean PMI, 19.3 hours) subjects. The concentration of cerebellar 3-NT, determined by ELISA, in controls ranged from 13.69 to 49.04 pmol g 1 of tissue; the concentration of 3-NT in autistic cases ranged from 3.91 to 333.03 pmol g 1 of tissue. Mean cerebellar 3-NT was elevated in autism by 68.9% and the increase was statistically significant (p=0.045). Cerebellar Hg, measured by atomic absorption spectrometry ranged from 0.9 to 35 pmol g 1 tissue in controls (n=10) and from 3.2 to 80.7 pmol g 1 tissue in autistic cases (n=9); the 68.2% increase in cerebellar Hg was not statistically significant. However, there was a positive correlation between cerebellar 3-NT and Hg levels (r=0.7961, p=0.0001). A small decrease in cerebellar Se levels in autism, measured by atomic absorption spectroscopy, was not statistically significant but was accompanied by a 42.9% reduction in the molar ratio of Se to Hg in the autistic cerebellum. While preliminary, the results of the present study add elevated oxidative stress markers in brain to the growing body of data reflecting greater oxidative stress in autism.

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Section: Implications Of Increased Cerebellar 3-nt In Autismmentioning

confidence: 99%

Oxidative Stress in Autism: Elevated Cerebellar 3-nitrotyrosine Levels

Sajdel-Sulkowska¹,

Lipiński²,

Windom³

et al. 2008

American J. of Biochemistry and Biotechnology

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“…[11][12][13][14][15]. Most of these conventional methods are very tedious, dependent on multi-step sample cleanup procedures, relatively expensive, and time consuming.…”

Section: Introductionmentioning

confidence: 99%

Selective detection of l-tyrosine in the presence of ascorbic acid, dopamine, and uric acid at poly(thionine)-modified glassy carbon electrode

Rahman

Lopa

Kim

et al. 2015

Journal of Electroanalytical Chemistry

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“…Acetaminophen (paracetamol, N-acetyl-p-aminophenol, AC) ( Figure 1b) is an extensively used antipyretic analgesic drug and a suitable alternative for patients who are sensitive to aspirin [13]. To date, various methods have been developed for the detection AC such as HPLC [14], spectrophotometry [15], and electrophoresis [16]. Alternatively, electrochemical methods have also received much interest due to their higher selectivity, lower cost, and faster operation than other methods.…”

Section: Introductionmentioning

confidence: 99%

Design of a New Carbon Paste Electrode Modified with TiO₂ Nanoparticles to Use in an Electrochemical Study of Codeine and Simultaneous Determination of Codeine and Acetaminophen in Human Plasma Serum Samples

Mashhadizadeh

Rasouli

2014

Electroanalysis

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A new electrochemical sensor was fabricated via TiO2 nanoparticles onto a carbon paste electrode. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) studied the response of the modified electrode toward codeine. The effects of pH, modifier amount, pulse amplitude, and scan rate of potential have been examined. Using DPV, we could measure simultaneously codeine and acetaminophen in one mixture. The detection limits of 0.018 and 0.050 µmol L−1 were achieved for codeine and acetaminophen, respectively. The electrooxidation pathway, transfer coefficient, and standard rate constant, are estimated. The proposed voltammetric sensor was successfully applied to determination of codeine and acetaminophen in human plasma serum samples.

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Determination of nitrotyrosine and tyrosine by high-performance liquid chromatography with tandem mass spectrometry and immunohistochemical analysis in livers of mice administered acetaminophen

Cited by 80 publications

References 25 publications

Oxidative Stress in Autism: Elevated Cerebellar 3-nitrotyrosine Levels

Oxidative Stress in Autism: Elevated Cerebellar 3-nitrotyrosine Levels

Selective detection of l-tyrosine in the presence of ascorbic acid, dopamine, and uric acid at poly(thionine)-modified glassy carbon electrode

Design of a New Carbon Paste Electrode Modified with TiO₂ Nanoparticles to Use in an Electrochemical Study of Codeine and Simultaneous Determination of Codeine and Acetaminophen in Human Plasma Serum Samples

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Determination of nitrotyrosine and tyrosine by high-performance liquid chromatography with tandem mass spectrometry and immunohistochemical analysis in livers of mice administered acetaminophen

Cited by 80 publications

References 25 publications

Oxidative Stress in Autism: Elevated Cerebellar 3-nitrotyrosine Levels

Oxidative Stress in Autism: Elevated Cerebellar 3-nitrotyrosine Levels

Selective detection of l-tyrosine in the presence of ascorbic acid, dopamine, and uric acid at poly(thionine)-modified glassy carbon electrode

Design of a New Carbon Paste Electrode Modified with TiO2 Nanoparticles to Use in an Electrochemical Study of Codeine and Simultaneous Determination of Codeine and Acetaminophen in Human Plasma Serum Samples

Contact Info

Product

Resources

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Design of a New Carbon Paste Electrode Modified with TiO₂ Nanoparticles to Use in an Electrochemical Study of Codeine and Simultaneous Determination of Codeine and Acetaminophen in Human Plasma Serum Samples