Determination of Nitrosamines in Sausages by Solid Phase Extraction with Activated Carbon – GC/MS

Abstract

This study shows a method for the detection and quantification of six volatile nitrosamines, dimethyl nitrosamine, diethylnitrosamine, N-nitrosopyrrolidine, N-nitrosopiperidine, N-nitrosomorpholine, N-nitrosoibuthylnitrosamine in meat products. The procedure based on: (a) Isolation of the compounds by vacuum steam distillation, (b) extraction in solid phase -SPE -from aqueous distillates using activated carbon and (c) analysis by gas chromatography-mass spectrometry. The recovery of the compounds from fortified samples (sausages and their preserved liquid) with 200 µg.Kg -1 ranged between 10.9 -61.7% to solid sample and 20.9 -80.4% to liquid sample. The direct application of this method to samples of canned sausages allowed the separation, identification and quantification of the nitrosamines at the µg.Kg-1 level with detection limit varying between 0.2 to 1.0 µg.Kg -1 . Gr up SMCopyright  Filho PJS with dispersive micro solid-phase extraction and gas chromatography-chemical ionisation mass spectrometry. Food Chem. 2013; 138: 227-233. 28. Sen NP, Seaman SW, Page BD. Rapid semi-quantitative estimation of N-nitrosodibutylamine and N-nitrosodibenzylamine in smoked hams by solid phase microextraction followed by gas chromatography-thermal energy analysis.

Anion exchange polymeric sorbent coupled to high‐performance liquid chromatography with UV diode array detection for the determination of ten N‐nitrosamines in meat products: a validated approach

“…Furthermore, although not comparable to mass spectrometry, the obtained limits of detection are suitable for developing accurate determinations of N ‐NAs levels in meats and as a tool for food safety controls. Indeed, different authors detected levels of some N ‐NAs (especially non‐volatile) in meat products, higher than the detection limits of this method (Herrmann et al ., 2015b; Sanches Filho et al ., ; Sallan et al ., ). It is also interesting to underline that the sensitivity reached in this study results are comparable with that of some of the other available methods (Sanches Filho et al ., ; Yuan et al ., ).…”

“…Due to high volatility of many N ‐NAs, this type of analytical determination is usually accomplished by using gas chromatography coupled to mass spectrometry. The sample preparation may be carried out by using steam distillation (Li et al ., ), ultrasonic solvent extraction (Yuan et al ., ), vacuum steam distillation/solid‐phase extraction (SPE) (Sanches Filho et al ., ) or double SPE (Jurtchenko et al ., ). High‐performance liquid chromatography was applied for identifying/quantifying also the non‐volatile N ‐NAs.…”