Determination of nitrophenols by capillary zone electrophoresis in a hydrodynamically closed separation compartment

Kaniansky, Dušan; Krčmová, Eva; Madajová, V.; Masár, Marián; Marák, Jozef; Onuska, Francis I.

doi:10.1016/s0021-9673(97)00102-7

Cited by 27 publications

(15 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The former concentration. level was close to that evaluated as a limit of determination of nitrophenols by CZE itself [18] for the same configuration of the CZE equipment as used in this work. This concentration level was chosen as it enabled us to perform comparative CZE experiments while developing the ITP pretreatment procedure.…”

Section: Itp Pretreatment Of Nitrophenols Present In Water Samplessupporting

confidence: 84%

“…Nitrophenols listed by the US Environmental Protection Agency in the group of priority phenol pollutants were separated by MEKC [15] and by capillary zone electrophoresis (CZE) [ 161. Recently, the separation potential of CZE for nitrophenols was investigated in details [17, 181. Here, using the CZE columns of 300 vm ID sample injection volumes could be enhanced so that concentration LODs in the range of 20-80 ppb were achieved for current photometric absorbance detection [18]. Such LOD values are linked with 200-800 ppb limits of quantitation [19] and, therefore, CZE often cannot be used directly for the determination of nitrophenols at concentrations of environmental or ecotoxicological relevance (see, e.g., [l-lo]).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Capillary zone electrophoresis of nitrophenols with off‐line isotachophoretic sample pretreatment

et al. 1997

Self Cite

View full text Add to dashboard Cite

Preparative capillary isotachophoresis (ITP) operating in a discontinuous fractionation mode was studied as a sample pretreatment technique for capillary zone electrophoresis (CZE) trace analysis of a group of ten nitrophenols. Different separation mechanisms employed by these capillary electrophoresis techniques made possible a group isolation of the studied analytes by ITP while their CZE resolutions based on differences in the interactions with polyvinylpyrrolidone (PVP) provided suitable conditions for a final analytical evaluation. Experiments with model and practical water samples revealed high and reproducible recovery rates of the ITP pretreatment of nitrophenols when sample loadability limits of the preparative ITP equipment were met. A main disadvantage of the studied ITP-CZE combination was an inefficient use of the pretreated sample as only 0.5% of the fraction containing nitrophenols was used in the final CZE step. Despite these limitations associated with the CZE sample injection, the concentration limit of detection (LOD) for nitrophenols in practical water samples could be reduced to 2-8 ppb concentrations (photometric absorbance detector operating at a 254 nm wavelength) when 200 microL sample volumes were taken for the ITP pretreatment. This was accompanied by an efficient sample clean-up as no other trace ionic constituents originating from the samples were detected on CZE.

show abstract

Section: Itp Pretreatment Of Nitrophenols Present In Water Samplessupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Capillary zone electrophoresis of nitrophenols with off‐line isotachophoretic sample pretreatment

et al. 1997

Self Cite

View full text Add to dashboard Cite

show abstract

“…2 illustrate also this aspect of the developed CZE procedure. In this context, we should note that such reproducibilities required that an adequate attention is paid to the minimization of fluctuations in the compositions and pH of the background electrolyte solutions due to carbon dioxide [49]. Means employed to reach such conditions are described in Section 2.4.…”

Section: Cze Monitoring Of Preparative Citp Separations Of Rhepomentioning

confidence: 99%

Fractionation of glycoforms of recombinant human erythropoietin by preparative capillary isotachophoresis

et al. 2005

Self Cite

View full text Add to dashboard Cite

This feasibility study deals with the use of preparative capillary isotachophoresis (CITP), operating in a discontinuous fractionation mode, to the separations and isolations of glycoforms of recombinant human erythropoietin (rhEPO). The preparative CITP separations were monitored by capillary zone electrophoresis (CZE) with a hydrodynamically closed separation unit. Such a CZE system, suppressing fluctuations of the migration data linked with fluctuations of EOF and hydrodynamic flow, made possible to evaluate and compare the preparative CITP separations performed within a longer time frame. Preparative CITP, carried out in the separation unit with coupled columns of enhanced sample loadability, separating 100 microg of rhEPO in a run lasting ca. 30 min, gave the production rate higher than 55 ng/s for the rhEPO glycoforms. The preparative separations included valve isolations of the glycoforms from the ITP stack into four or six fractions. Such numbers of the fractions corresponded to typical numbers of the major glycoform peaks as resolved in CZE of rhEPO. With respect to close effective mobilities of the glycoforms and a multicomponent nature of rhEPO, the fractions contained mixtures of glycoforms with the dominant glycoforms enriched 10-100-fold, relative to the original rhEPO sample.

show abstract

“…13 Although sensitive and selective, these methods depend on multi-step sample clean-up procedures and are, therefore, generally time-consuming and require skilled personnel. Electrochemical method, which can offer fast, simple and direct real time analysis is one of the most used techniques in the determination of phenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

A Novel Sensor Based on Electropolymerization Poly(safranine) Film Electrode for Voltammetric Determination of 4-Nitrophenol

Liu¹

2010

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

A novel poly(safranine)-modified electrode has been constructed for the determination of 4-nitrophenol (4-NP) in natural water sample. The electrochemical behavior of poly(safranine) film electrode and its electrocatalytic activity toward 4-NP were studied in detail by cyclic voltammetry (CV) and adsorptive linear stripping voltammetry (LSV). All experimental parameters were optimized and LSV was proposed for its determination. In optimal working conditions, the reduction current of 4-NP at this poly ( . The high sensitivity and selectivity of the sensor were demonstrated by its practical application for the determination of trace amounts of 4-NP in natural water and fruit samples.

show abstract

Determination of nitrophenols by capillary zone electrophoresis in a hydrodynamically closed separation compartment

Cited by 27 publications

References 28 publications

Capillary zone electrophoresis of nitrophenols with off‐line isotachophoretic sample pretreatment

Capillary zone electrophoresis of nitrophenols with off‐line isotachophoretic sample pretreatment

Fractionation of glycoforms of recombinant human erythropoietin by preparative capillary isotachophoresis

A Novel Sensor Based on Electropolymerization Poly(safranine) Film Electrode for Voltammetric Determination of 4-Nitrophenol

Contact Info

Product

Resources

About