Determination of Nitrofurans Residue in Fishery Products Using High Performance Liquid Chromatography

Abstract

Nitrofurans residue in fishery products were dangerous for human health and still remain a big challenge in China. However, there are a few available methods to detect nitrofurans residue in fishery products. This study aimed at developing a high performance liquid chromatography (HPLC) method to detect four nitrofurans (furaltadone, nitrofurazone, nitrofurantoin and furazolidone) residue in fishery products sensitively . Firstly the nitrofurans were extracted from samples with ethyl acetate and then concentrated with rotary evaporator. After mobile phase dissolved and normal hexane defatted, the analytes were quantified with an external standard method by HPLC. The results showed that the linear range was 0.025~1.000μg/mL with correlation coefficients all more than 0.998 and the quantification limits of furaltadone, nitrofurazone, nitrofurantoin, furazolidone, were 11.0, 6.00, 13.0 and 7.00μg/kg respectively. The method was used to detect the nitrofurans in fishery products prepared with recovery rates from 72.2% to 107.4% and relative standard deviation (RSD) varied from 6.2% to 10.4%. Therefore, this method can be used for monitoring nitrofurans in commercial fishery products, improving the safety of commercial fishery products in China.

Nitrofuran Determination in Aquaculture Water by Using Poly-Alizarin Red-Modified Electrode

“…7d, the peak width half of reduction and oxidation peaks was 58. I pc (μA) = −0.58173 ν 0.5 + 0.44108 R 2 = 0.95952 [5] This result indicated that the oxidation process was more susceptible to adsorption control, and the reduction process was controlled by diffusion.…”

“…At present, the detection methods of NF include high performance liquid chromatography (HPLC), [4][5][6] liquid chromatography-tandem mass spectrometry (LC-MS), [7][8][9] enzyme-linked immunosorbent assay (ELISA), 10 and electrochemical methods. Among these methods, HPLC is sensitive, accurate, and repeatable, but the pretreatment is cumbersome, and the instrument is expensive.…”