Determination of Nitric Oxide by Quenching Electro‐Chemiluminescence of Tris(2,2′‐Bipyridyl)ruthenium in Flow Injection Analysis

Abstract

Quenching of the electro-chemiluminescence of the ruthenium complex was applied for detection of NO at the flow injection apparatus equipped with a homemade gas pressure pump with low noise and a flow-through electrogenerated chemiluminescence (ECL) cell. NO quenched the ECL emission of Ru(bpy) 3 2þ at the potential of the oxidation of Ru(bpy) 3 2þ efficiently to set the detection limit of NO at 0.1% of concentration of Ru(bpy) 3 2þ . The decrease in ECL intensity is linearly dependent on the logarithmic concentration of NO in the range of 5.7 Â 10 À7 M to 1.8 Â 10 À5 M. Voltammetric and spectrometric analysis showed that NO was not coordinated to Ru(bpy) 3 2þ/3þ , but catalyzed the quenching reaction.

A ruthenium(ii) complex based turn-on electrochemiluminescence probe for the detection of nitric oxide

“…In 2007, Chen et al reported the ECL detection of NO using a [Ru (bpy) 3 ] 2+ /TPrA system based on the ECL quenching of NO to the system. 15 Compared to this report, our method shows the better results including a wider linear range and a lower detection limit.…”

“…However, at high concentration levels it can rapidly react with other reactive oxygen species (ROS) to form reactive nitrogen species (RNS) to cause the damage of the cells. 4 Therefore, the detection of NO is an attractive work for understanding the complicated functions of NO in living systems, and a number of methods, such as fluorescence, [5][6][7][8][9][10] chemiluminescence, 11 electron paramagnetic resonance spectroscopy, 3,[12][13][14] and electrochemiluminescence, 15 have been developed for the detection of NO.…”

Flow injection analysis for nitric oxide quantification based on reduced fluoresceinamine

“…Some previous analytical methodologies were presented for the direct NO quantification by FIA. [14][15][16][17][18][19][20][21] Most of them are based on the chemiluminescence analytical technique. One is based on the quenching of the electro-chemiluminescence of a ruthenium complex 14 and all the others are based on the oxidation of luminol by hydrogen peroxide.…”

“…[14][15][16][17][18][19][20][21] Most of them are based on the chemiluminescence analytical technique. One is based on the quenching of the electro-chemiluminescence of a ruthenium complex 14 and all the others are based on the oxidation of luminol by hydrogen peroxide. [15][16][17][18][19][20] In these methodologies different procedures were used for the preparation of NO standard solutions and in the majority the NO is sampled by microdialysis and/or retained by different kinds of compounds.…”

Development and application of an electrochemiluminescent flow-injection cell based on CdTe quantum dots modified electrode for high sensitive determination of dopamine

“…During past decades, considerable attention has been paid to develop the ECL-flow injection analytical (FIA) system. [19][20][21][22][23][24][25] Especially the miniaturized ECL flow cell has aroused the interest from researchers. [26][27][28][29] .…”