Determination of Nifedipine in plasma by a rapid capillary gas chromatographic method

Abstract

A rapid, specific and reliable gas chromatographic assay procedure for Nifedipine in plasma has been developed. With a single-step solvent extraction, and electron capture detection, the method is sensitive to 0.5 ng/mL of plasma and the standard curve is linear from 0.5 to 500 ng/mL. Samples are protected from light to prevent formation of photodecomposition products. The method has been used to monitor drug concentrations in patients receiving therapeutic doses.

Determination of nifedipine in human plasma by ultra performance liquid chromatography–tandem mass spectrometry and its application in a pharmacokinetic study

“…Several analytical methods based on gas chromatography (GC) [6][7][8] and high performance liquid chromatography (HPLC) [9][10][11][12][13][14][15] have been reported for the determination of nifedipine in biological samples. Although some GC methods could provide high sensitivity for the pharmacokinetic study, thermal decomposition of nifedipine under GC condition was the major problem.…”

Rapid and simultaneous determination of nifedipine and dehydronifedipine in human plasma by liquid chromatography–tandem mass spectrometry: Application to a clinical herb–drug interaction study

“…Most of these reported analytical methods involve the application of gas chromatography (GC) and liquid chromatography (LC). The reported GC methods often utilize electron-capture detection [18,[26][27][28][29][30], flame ionization detection [31], nitrogen-phosphorus detection [32], or mass spectrometric (MS) detection [33,34]. Although the sensitivity of GC/MS is high in pharmacokinetic and pharmacogenetic studies, the GC method has several concerning drawbacks, such as thermal degradation of NIF and DNIF due to high temperatures during analysis.…”

Cathodic adsorptive stripping square-wave voltammetric determination of nifedipine drug in bulk, pharmaceutical formulation and human serum

“…Several analytical methods have been described for the assay of nifedipine in pharmaceutical formulations and biological fluids. These methods include spectrophotometry [3,4,5,6], gas chromatography [5,6,7,8,9,10,11,12,13,14], liquid chromatography [6,15,16], high-performance thin-layer chromatography [17], high-performance liquid chromatography [18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41], flow-injection tandem mass spectrometry [42] polarography [43,44], linear-sweep voltammetry [45] and differential-pulse stripping voltammetry [46,47].…”