Determination of neutral sugars in soil by capillary gas chromatography after derivatization to aldononitrile acetates

Determination of carbohydrates in tobacco by pressurized liquid extraction combined with a novel ultrasound-assisted dispersive liquid–liquid microextraction method

“…Therefore, a reaction time of 5 min was selected to conduct both the oximation and acetylation. Compared with the literature method, the optimized method provided similar reaction efficiency, less reaction time, and milder reaction conditions (Table 2S) [30]. The new catalyst was further evaluated by studying its tolerance to different volumes of water (0, 25, 50, 80, and 100 μL).…”

Derivatization of carbohydrates for GC and GC–MS analyses

“…After hydrolysis with TFA the sugars were converted into aldononitrile acetate derivatives. In comparison with TMS derivatives, the aldononitrile acetates were more stable, gave better recovery, and the reproducibility of ribose analysis improved significantly [29].…”

“…However, the validity of quantitative analysis using these derivatives has been called into question [28], and they cannot be applied for ketoses [26]. Zhang et al [29] developed a method for the simultaneously quantitation of ribose, rhamnose, arabinose, xylose, fucose, mannose, glucose, and galactose in soil by GC. After hydrolysis with TFA the sugars were converted into aldononitrile acetate derivatives.…”

Elevated O3 and wheat cultivars influence the relative contribution of plant and microbe-derived carbohydrates to soil organic matter

“…Soil neutral sugars were determined according to the method of Zhang et al (2007). Briefly, air-dried soil samples were hydrolyzed with 4 M trifluoroacetic acid (TFA) and the hydrolyte was filtered.…”

“…Changes in the quantity or quality of carbon flux into the soil will influence the interactions among soil organisms (Li et al, 2012;Li et al, 2013) and then alter carbon retention and mineralization in soil ecosystem (Andersen, 2003). As the labile fraction of SOM, soil carbohydrates account for about 5-25% of total SOM (Stevenson, 1994;Zhang et al, 2007). Soil carbohydrates are highly responsive to changes in C inputs to the soil because of their fast turnover rates (Schmitt and Glaser, 2011).…”