Determination of Neonicotinoid Insecticides in Bee Products by Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Valverde, Silvia; Ares, Ana M.; Nozal, Marı́a J.; Bernal, José

doi:10.1007/978-1-0716-1928-5_2

Cited by 3 publications

(10 citation statements)

References 42 publications

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“…Sample treatment is a crucial step when developing sample preparation methods. Considering bee products and given the diverse matrix components present, it has become imperative to choose different approaches focused on a specific api-product [19]. These methodologies encompass not only the extraction of analytes but also the implementation of effective sample clean-up steps.…”

Section: Sample Preparation Methods To Study Pesticides In Royal Jellymentioning

confidence: 99%

“…Additionally, it is also of utmost importance to consider the physical state of royal jelly, as it can be found in various states [43], which significantly impact the development of sample preparation methods. For example, two methodologies have been developed for analyzing neonicotinoids in products based on royal jelly, one for fresh royal jelly [3], and another for liquid dietary supplements, specifically lyophilized royal jelly in the form of ampoules [19]. Another factor affecting the sample treatment of the royal jelly is its composition, so the extraction solvent and clean-up steps should be optimized to remove sugar and protein interferents that can affect chromatography measurements [47].…”

Section: Sample Preparation Methods To Study Pesticides In Royal Jellymentioning

confidence: 99%

“…However, the utilization of neonicotinoid active substances in the seeds of crops such as cotton, corn, and sunflowers has sparked a hypothesis linking this particular class of pesticides to the occurrence of colony collapse disorder syndrome [10]. Different neonicotinoids have been studied in royal jelly [3,19,34,49], due to the potential presence in crops visited by bees, but in most cases, no neonicotinoids were detected. Seven neonicotinoids (dinotefuran, nitenpyram, thiamethoxam, clothianidin, imidacloprid, acetamiprid and thiacloprid) were investigated in royal jelly-based products from different Spanish regions (n = 12), but no residues were detected [3].…”

Section: Insecticidesmentioning

confidence: 99%

“…While pesticide residue determination in honey, bee pollen and beeswax has been extensively studied [18], there is a scarcity of publications specifically addressing pesticides in royal jelly [11,13,19,20]. The first article for pesticide analysis in royal jelly was published in the literature in 2001 [21].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Contamination of Honeybee (Apis mellifera L.) Royal Jelly by Pesticides and Sample Preparation Methods for Its Determination: A Critical Appraisal

Fuente-Ballesteros,

Brugnerotto,

Nguyen

et al. 2023

Foods

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Pesticides can easily enter the food chain, harming bee populations and ecosystems. Exposure of beehive products to various contaminants has been identified as one of the factors contributing to the decline in bee populations, and multiple food alerts have been reported. Despite this fact, royal jelly, a valuable bee product with nutritional and functional properties, has received less attention in this context. Pesticide residues of different chemical class can contaminate royal jelly when foraging bees collect pollen or nectar from pesticide-treated flowers, or in some cases, due to its frequent and inappropriate use in the treatment of mites in beehives. To monitor this issue and also make it more reliable, it is crucial to develop effective sample preparation methods for extracting pesticides from royal jelly for subsequent analysis. In this context, this review provides information about sample preparation methods (solid-phase extraction, solvent extraction, and QuEChERS—quick, easy, cheap, effective, rugged and safe) and analytical methods that have been validated or improved to extract and analyze pesticides, respectively, in royal jelly samples of different origins. Finally, future perspectives are discussed. With this background, we aim to provide data that can guide future research related to this topic.

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Section: Sample Preparation Methods To Study Pesticides In Royal Jellymentioning

confidence: 99%

Section: Sample Preparation Methods To Study Pesticides In Royal Jellymentioning

confidence: 99%

Section: Insecticidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Contamination of Honeybee (Apis mellifera L.) Royal Jelly by Pesticides and Sample Preparation Methods for Its Determination: A Critical Appraisal

Fuente-Ballesteros,

Brugnerotto,

Nguyen

et al. 2023

Foods

View full text Add to dashboard Cite

show abstract

“…Liquid-liquid extraction (LLE) [8], solid-phase extraction (SPE) [9,10], QuEChERS extraction [11][12][13], and dispersive liquid-liquid microextraction (DLLME) [14] can be counted among the efective extraction methods used. A high-pressure liquid chromatography diode array detector (HPLC/DAD) [15], a gas chromatography-mass spectrometer (GC-MS/MS) [13], and liquid chromatography-mass spectrometry (LC-MS/MS) are used for neonicotinoid analyses [12,16].…”

Section: Introductionmentioning

confidence: 99%

Neonicotinoid Analysis in Sunflower (Helianthus annuus) Honey Samples Collected around Tekirdag in Turkey

Özdemir

Muz

2023

International Journal of Analytical Chemistry

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In recent years, the widespread use of neonicotinoids in agricultural areas has caused environmental pollution due to its lower toxicity to mammals. Honey bees, which are considered as biological indicators of environmental pollution, can carry these pollutants to the hives. Forager bees returning from sunflower crops that have been treated with neonicotinoids treated sunflower fields cause residue accumulation in the hives, which reason colony-level adverse effects. This study analyses neonicotinoid residues in sunflower (Helianthus annuus) honey sampled by beekeepers from Tekirdag province. Honey samples have been subjected to liquid-liquid extraction methods before liquid chromatography-mass spectrometry (LC-MS/MS). The method validation was carried out to fulfill all the necessary requirements of procedures SANCO/12571/2013. Accuracy was in the range of 93.63–108.56%, for recovery in the range of 63.04–103.19%, and for precision in the range 6.03–12.77%. Detection and quantification limits were determined according to the maximum residue limits of each analyte. No neonicotinoid residues were found above the maximum residue limit in the sunflower honey samples analysed.

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Luminescent Probe Based on Terbium-Carbon Quantum Dots for the Quantification of Imidacloprid in Caneberries

Llorent-Martínez,

Jiménez-López,

Ruiz-Medina

2023

Journal of Analytical Methods in Chemistry

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We propose a modification of terbium-sensitized luminescence (TSL) by means of the introduction of nanoparticles to improve the sensitivity and selectivity of the analytical methods. TSL detection is usually based on the complexation between fluorescent organic compounds (the analytes) and terbium. The organic compound is then excited, and, after an energy transfer towards terbium, the latter emits the luminescence signal. Here, the modification consists of the introduction of nanoparticles (carbon quantum dots, CQDs) into the system. The carboxylic groups of CQDs react with terbium, providing an interesting time-resolved luminescence probe. We applied this system for the determination of the neonicotinoid imidacloprid (IMID). When IMID was introduced in the terbium-CQDs system, the luminescent signal (λexc/λem of 256/545 nm) was quenched, proportionally to IMID concentration in the range of 100–2500 ng·mL−1, obtaining a limit of detection of 30 ng·mL−1. A method detection limit of 0.9 mg·kg−1 was reached in caneberries, thus complying with the maximum residue level of 5 mg·kg−1 established by Codex Alimentarius. We performed recovery experiments in caneberries (blackberries, blueberries, raspberries, and mulberries), obtaining recovery yields close to 100% in all cases. These results show that the use of terbium ions-nanoparticles luminescence probes can be useful for screening purposes in quality control laboratories.

show abstract

Determination of Neonicotinoid Insecticides in Bee Products by Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Cited by 3 publications

References 42 publications

Contamination of Honeybee (Apis mellifera L.) Royal Jelly by Pesticides and Sample Preparation Methods for Its Determination: A Critical Appraisal

Contamination of Honeybee (Apis mellifera L.) Royal Jelly by Pesticides and Sample Preparation Methods for Its Determination: A Critical Appraisal

Neonicotinoid Analysis in Sunflower (Helianthus annuus) Honey Samples Collected around Tekirdag in Turkey

Luminescent Probe Based on Terbium-Carbon Quantum Dots for the Quantification of Imidacloprid in Caneberries

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