Determination of Neisseria gonorrhoeae susceptibility to ciprofloxacin in clinical specimens from men using a real-time PCR assay

Magooa, Mahlape Precious; Müller, Etienne; Gumede, Lindy; Lewis, David A.

doi:10.1016/j.ijantimicag.2013.02.026

Cited by 23 publications

(21 citation statements)

References 10 publications

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“…The 40 isolates consisted of 11 susceptible isolates, 2 isolates of intermediate susceptibility and 27 resistant isolates. Twenty-four of these linked first-pass urine samples were obtained from men presenting with urethral gonorrhoea [8]. Each gonococcal isolate in the study was reidentified based on Gram staining-a positive oxidase test (Davies Diagnostics, Randburg, South Africa) and coagglutination with the Phadebact® Monoclonal Antibody Test (Pharmacia Diagnostics AB, Uppsala, Sweden).…”

Section: Determining the Antibiotics Resistance Of Pathogen Std Agentsmentioning

confidence: 99%

“…DNA extracted from first-pass urine of 15 out of 16 patients with ciprofloxacin-resistant gonorrhoea generated no gyrA and parC amplicons. The modified RT-PCR assay previously described in this paper offered a faster method for combined N. gonorrhoeae diagnosis and determination of ciprofloxacin susceptibility than traditional culture and antimicrobial resistance testing [8].…”

Section: Determining the Antibiotics Resistance Of Pathogen Std Agentsmentioning

confidence: 99%

“…There are multiple mechanisms leading to antibiotic resistance, one involving resistance plasmids carrying genes responsible for resistance to antibiotics [5,6]. The phenomenon of antibiotic resistance can also develop via mutations or by acquiring resistance genes from other resistant bacteria [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…N. gonorrhoeae are Gram-negative facultative intracellular bacteria accounting for about three-quarters of urethral discharge cases among South Africa men presenting to primary healthcare clinics [8]. Chlamydia and gonorrhoea share several aspects of their disease outcomes, both being able to cause pelvic inflammatory disease and epithelial scarring which can lead to infertility.…”

Section: Introductionmentioning

confidence: 99%

“…The increasing prevalence of N. gonorrhoeae antimicrobial resistance has become a global public health problem as lesser abilities to develop N. gonorrhoeae cultures and to perform antimicrobial susceptibility testing make monitoring antimicrobial resistance rather difficult. A significant advantage to public health programs and selective patient management could be provided by the use of molecular assays for gonococcal antimicrobial resistance, particularly in the case of fluoroquinolones [8].…”

Section: Introductionmentioning

confidence: 99%

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Determining the Antibiotic Resistance of Bacterial Pathogens in Sexually Transmitted Diseases

Laura¹,

Vladi²,

Vasile³

2017

Antibacterial Agents

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Sexually transmitted diseases (STD) are among the most common infections worldwide. These bacterial infections have spread predominantly in the developing/underdeveloped countries, the most common being syphilis, gonorrhea and those induced by Chlamydia trachomatis, Ureaplasma urealyticum or Mycoplasma spp. Due to extensive usage of antibiotics in the recent past, these bacteria developed resistance to those commonly used for treatment, such resistant strains becoming a public health problem in a number of countries. It is well documented that bacterial STD agents are difficult to detect using standard culture media because these methods require special conditions and adequate nutrients. Antimicrobial susceptibility testing is, therefore, difficult to obtain in such cases. In recent years, genetic tests have been frequently employed in STD diagnosis. The study of genes that induce resistance to antibiotics using DNA isolated from these bacteria may prove to be a viable alternative. Genetic methods enable the DNA extraction from different biological samples, and both the presence of the bacteria and their resistance to one or more antibiotics can be determined from a single DNA sample. By studying the genes that induce antibiotic resistance and the plasmids that transfer such genes, the mechanism that leads to antibiotic resistance can be elucidated.

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Section: Determining the Antibiotics Resistance Of Pathogen Std Agentsmentioning

confidence: 99%

Section: Determining the Antibiotics Resistance Of Pathogen Std Agentsmentioning

confidence: 99%