Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains

Petersen, Bent O.; Meier, Sebastián; Paulsen, Berit Smestad; Redondo, Antonio R.; Skovsted, Ian C.

doi:10.1016/j.carres.2014.06.018

Cited by 11 publications

(8 citation statements)

References 20 publications

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“…To determine the positions of O-acetylation of the backbone of the native CPS42, we used the carbonyl HMBC spectra to show that there are two forms of the CPS: one form with O-acetylation at both A5 and A6 and a second form with acetylation only at A6, consistent with published results (17). Our data indicated, however, that there is a third form that has no O-acetylation and that is thus identical to CPS35C.…”

Section: Structure Of Cps35fsupporting

confidence: 82%

“…Additional WcrH acceptors may thus be suggested from the structures of CPS36 and CPS43, the only serotypes in genetic cluster 4 (9) that remain to be structurally characterized. CR931721, CR931703, CR931704, CR931706, CR931715, CR931705, and CR931694) (A) with the corresponding CPS structures determined in the present or previous investigations (10,11,16,17,19) (B). The antigenic formulas of the CPS serotypes are indicated in parentheses.…”

Section: Discussionmentioning

confidence: 73%

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Structures of Capsular Polysaccharide Serotypes 35F and 35C of Streptococcus pneumoniae Determined by Nuclear Magnetic Resonance and Their Relation to Other Cross-Reactive Serotypes

2015

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The structures of Streptococcus pneumoniae capsular polysaccharides (CPSs) are essential for defining the antigenic as well as genetic relationships between CPS serotypes. The four serotypes that comprise CPS serogroup 35 (i.e., types 35F, 35A, 35B, and 35C) are known to cross-react with genetically related type 20, 29, 34, 42, or 47F. While the structures of CPS serotype 35A (CPS35A) and CPS35B are known, those of CPS35F and CPS35C are not. In the present study, the serotypes of CPS35F and CPS35C were characterized by high-resolution heteronuclear magnetic resonance (NMR) spectroscopy and glycosyl composition analyses to reveal the following repeat unit structures:Glc␣1 where OAc indicates O-acetylated. Importantly, CPS35F, the immunizing serotype for the production of group 35 serum, more closely resembles CPS34 and CPS47F than other members of serogroup 35. Moreover, CPS35C is distinct from either CPS35F or CPS35B but closely related to CPS35A and identical to de-O-acetylated CPS42. The findings provide a comprehensive view of the structural and genetic relations that exist between the members of CPS serogroup 35 and other cross-reactive serotypes. T he virulence of Streptococcus pneumoniae depends in part on the production of capsular polysaccharides (CPSs) that protect invading bacteria from phagocytosis and subsequent killing by host neutrophils (1). When administered in vaccines, these polysaccharides elicit the formation of opsonic antibodies that confer serotype-specific protective immunity. Currently available CPS-based vaccines include the 23-valent polysaccharide vaccine and the 13-valent conjugate vaccine. The widespread use of these vaccines, while highly effective in preventing invasive pneumococcal disease, may also favor the emergence of nonvaccine, replacement serotypes (2) or possible new serotypes by recombination of existing strains (3). Surveillance of both vaccine and nonvaccine pneumococcal serotypes is thus essential for monitoring and maintaining vaccine efficacy. IMPORTANCE Cross-reactions of diagnostic rabbit antisera withTwenty-four of the 92 presently recognized CPS serotypes (4-6) are distinct in the Danish system of nomenclature, while the remaining 68 are distributed among 21 serogroups that contain from two to five members each. For the most part, cross-reactions between serotypes are limited to members of the same CPS serogroup. Exceptions do, however, occur, most notably among a group of nonvaccine serotypes (7) that includes the members of CPS serogroup 35 (i.e., 35F, 35A, 35B, and 35C) and other individual types (i.e., 20, 29, 34, 42, and 47F). Examples of crossreactions between these types include group 35 serum with types 42 and 47F (8), factor 34b serum with type 35F, factor 20b serum

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Section: Structure Of Cps35fsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 73%

Structures of Capsular Polysaccharide Serotypes 35F and 35C of Streptococcus pneumoniae Determined by Nuclear Magnetic Resonance and Their Relation to Other Cross-Reactive Serotypes

2015

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“…Pool G typing antiserum, which recognizes serotypes 35C and 42 (15), strongly stained all putative 35C and 42 isolates. Each isolate was positive for factor sera 35c and 42a, whereas binding by factor sera 35b and 29b was at or below the levels of the irrelevant serotype (12,13). The wciG genes of all isolates serologically classified as serotype 35C encoded an intact WciG (see Table 2 for accession numbers).…”

Section: Resultsmentioning

confidence: 99%

“…2A]) (11). However, biochemical studies showed that serotype 42 capsular polysaccharide (CPS) has two O-acetylations on its 3-linked ␤-galactofuranose (Galf) (12,13), while a commercial serotype 35C capsule preparation contained no O-acetylation (13). In addition, both serotypes are known to bind ficolin-2, which targets O-acetyl groups in some pneumococcal serotypes (14).…”

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confidence: 99%

WciG O -Acetyltransferase Functionality Differentiates Pneumococcal Serotypes 35C and 42

et al. 2017

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Streptococcus pneumoniae expresses capsular polysaccharides (CPSs) to protect itself from opsonophagocytic killing. The genes responsible for capsules synthesized by the Wzy-dependent mechanism, which accounts for 96 of the 98 known pneumococcal capsule types, are in a chromosomal region known as the cps locus. The nucleotide sequence in this region has been determined for all serotypes. In contrast, not all CPS structures have been defined. The structure of the serotype 35C polysaccharide was recently reported, but the presence of O-acetyltransferase genes in the serotype 35C cps locus suggested that it could be incomplete, as the reported structure contains no O-acetylation. In addition, the genetic distinction of serotype 35C from the closely related serotype 42 was unclear, as their reported cps loci are nearly identical. To clarify these discrepancies, we obtained serotype 35C and 42 clinical and reference isolates and studied their serological and genetic properties, as well as the structures of CPSs purified from reference isolates. We demonstrated that the Oacetyltransferase WciG was functional in serotype 35C but nonfunctional in serotype 42 due to a deletion in wciG. Serotype 35C was O-acetylated at the 5-and 6-positions of 3-␤-galactofuranose, as well as the 2-position of 6-␤-galactofuranose. However, serotype 42 has only O-acetylation at 3-␤-galactofuranose, an observation consistent with its loss of WciG functionality, which is associated with O-acetylation at the 2-position and subsequent reaction with typing antiserum 35a. These findings provide a comprehensive view of the genetic, biochemical structural, and serological bases of serotypes 35C and 42.

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“…In the anomeric region, four major signals appeared at ı 5. Table 2 and were obtained from NMR analytical techniques and reference data [28][29][30][31][32]. On this basis, a series of possible linkage sites and sequence among residues were simulated by MestReNova and ChemNMR soft-ware.…”

Section: Structural Analysismentioning

confidence: 99%

Characterization and bioactivities of a novel purified polysaccharide from Endothelium corneum gigeriae Galli

Xiong

Jing

et al. 2015

International Journal of Biological Macromolecules

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Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains

Cited by 11 publications

References 20 publications

Structures of Capsular Polysaccharide Serotypes 35F and 35C of Streptococcus pneumoniae Determined by Nuclear Magnetic Resonance and Their Relation to Other Cross-Reactive Serotypes

Structures of Capsular Polysaccharide Serotypes 35F and 35C of Streptococcus pneumoniae Determined by Nuclear Magnetic Resonance and Their Relation to Other Cross-Reactive Serotypes

WciG O -Acetyltransferase Functionality Differentiates Pneumococcal Serotypes 35C and 42

Characterization and bioactivities of a novel purified polysaccharide from Endothelium corneum gigeriae Galli

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