Determination of naphthalene-2,3-dicarboxaldehyde-labeled amino acids by open tubular liquid chromatography with electrochemical detection

Oates, Mary D.; Jorgenson, James W.

doi:10.1021/ac00180a011

Cited by 74 publications

(30 citation statements)

References 25 publications

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“…Therefore, fluorescence probes are extensively used in physical, chemical and biological sciences for investigating the structure and dynamics of living system [13,14]. Other techniques used to improve detection limits are micro-column LC [15] and CE [16]. At present, popular methods for the determination of aliphatic amines are pre-column and post-column derivatization with fluorescence detection.…”

Section: Introductionmentioning

confidence: 99%

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

You

Zhao

Sun

et al. 2009

J of Separation Science

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Original PaperApplication of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS A simple, sensitive method for the determination of aliphatic amines based on a sulfonylation reaction using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as pre-column labeling reagent with fluorescence detection and APCI-MS identification has been developed. The labeled derivatives exhibited high stability and were enough to be efficiently analyzed by HPLC with an excitation maximum at k ex 270 nm and an emission maximum at k em 430 nm. Identification of derivatives was carried out by online post-column MS in positive-ion mode. Comparing with the widely used 5-dimethylaminonaphthalene-1-sulfonylchloride (Dansyl-Cl), EASC-amine derivatives not only exhibited high fluorescence but also exhibited excellent MS ionizable potential. Detection limits obtained from 0.10 pmol injection, at a S/N of 3, were 4.0 -12.7 fmol. The mean intra-and inter-assay precision for all aliphatic amine levels were a3.84 and 3.21%, respectively. Excellent linear responses were observed with coefficients of A0.9995.

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Section: Introductionmentioning

confidence: 99%

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

You

Zhao

Sun

et al. 2009

J of Separation Science

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“…Electrochemical detection circumvents the problem of fluorescence quenching from amino acid derivatives. Detection limits in the subfemtomole region have been reported for NDA derivatives with electrochemical detection [16]. These levels are even lower than those reported for laser-induced fluorescence detection [12].…”

Section: Introductionmentioning

confidence: 78%

Selective Analysis of Secondary Amines Using Liquid Chromatography with Electrochemical Detection (LC‐EC)

Williams

Koppang

2006

Electroanalysis

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In a mixture of primary and secondary aliphatic amines, the primary amines were derivatized (masked) with ophthalaldehyde (OPA) followed by derivatization of the remaining secondary amines with ferrocenecarboxylic acid chloride (FAC). The "tagged" amines were analyzed by LC-EC (liquid chromatography with electrochemical detection) using in-series dual electrode detection. Chemically-reversible oxidation of the FAC tagged secondary amines and their subsequent complementary oxidation and reduction signals coupled with chemically-irreversible oxidation of OPA tagged primary amines provided the selectivity for quantitative secondary amine analysis. The procedure was also applied for the selective identification of fragment 4 -11 (N-terminus-proline) of Substance P in the presence of other Substance P fragments with primary amino acids as their N-termini.

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“…31 NDA has been used as catecholamines ͑e.g., dopamine, norepinephrine͒, labeled reagent in single cell analysis by capillary electrophoresis. 32 In this study we used NDA to stain SH-SY5Y neuroblastoma cells.…”

Section: A Fluorescence Imaging Evaluationmentioning

confidence: 99%

Release monitoring of single cells on a microfluidic device coupled with fluorescence microscopy and electrochemistry

et al. 2010

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A method for monitoring the biological exocytotic phenomena on a microfluidic system was proposed. A microfluidic device coupled with functionalities of fluorescence imaging and amperometric detection has been developed to enable the real-time monitoring of the exocytotic events. Exocytotic release of single SH-SY5Y neuroblastoma cells was studied. By staining the cells located on integrated microelectrodes with naphthalene-2,3-dicarboxaldehyde, punctuate fluorescence consistent with localization of neurotransmitters stored in vesicles was obtained. The stimulated exocytotic release was successfully observed at the surface of SH-SY5Y cells without refitting the commercial inverted fluorescence microscope. Spatially and temporally resolved exocytotic events from single cells on a microfluidic device were visualized in real time using fluorescence microscopy and were amperometrically recorded by the electrochemical system simultaneously. This coupled technique is simple and is hoped to provide new insights into the mechanisms responsible for the kinetics of exocytosis.

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Determination of naphthalene-2,3-dicarboxaldehyde-labeled amino acids by open tubular liquid chromatography with electrochemical detection

Cited by 74 publications

References 25 publications

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

Application of 10‐ethyl‐acridine‐3‐sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI‐MS

Selective Analysis of Secondary Amines Using Liquid Chromatography with Electrochemical Detection (LC‐EC)

Release monitoring of single cells on a microfluidic device coupled with fluorescence microscopy and electrochemistry

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