Determination of N-acetylcysteine via its effect on the aggregation of gold nanoparticles

Gold nanoparticles as a colorimetric probe for the determination of N-acetyl-l-cysteine in biological samples and pharmaceutical formulations

“…Under the proposed conditions, increasing the NAC concentration results in a decrease in the plasmon absorbance without any wavelength shi, allowing for analyte quantication based on the absorption at a single wavelength. In comparison with another method based on AuNPs, 22 the proposed approach is convenient, low-cost and free of complex equipment, making it possible to analyse NAC in biological samples and pharmaceutical formulations.…”

“…Therefore, use of AuNPs is feasible for applications in various elds such as biosensors, nanomedicine, separation science, cell imaging and catalysis. 21 Sierra-Rodero et al 22 studied the effect of NAC on the kinetics of AuNP aggregation using a stopped-ow mixing technique together with light scattering detection. The signal obtained was measured aer about 5 s and gave the analytical information for a calibration graph in the NAC concentration range of 2.9 to 60 mmol L À1 with a limit of detection (LOD) of 0.87 mmol L À1 .…”

Use of metal nanoparticles for preconcentration and analysis of biological thiols

“…In this section, other optical methods used for biothiol analysis with NPs are summarized. Light scattering detection with stopped-flow mixing technique was used for the determination of NAC, based on the kinetic effect on aggregation of AuNPs in the presence of CTAB [135]. RLS of FSN-capped AuNPs was used for the detection of Cys and Hcys [136].…”