Determination of N-acetylcysteine in human plasma by liquid chromatography coupled to tandem mass spectrometry

Celma, C.; Allué, J.A; Pruñonosa, J; Peraire, Concepción; Obach, R.

doi:10.1016/s0021-9673(99)01078-x

Cited by 30 publications

(29 citation statements)

References 11 publications

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“…In biological fluids, HPLC has been the most predominant technique for the quantification of ACT and fluorescence detection was applied by using different reagents such as N-(1-pyrenyl) maleimide [29] or ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F) [30] as precolumn fluorescence labeling reagents while ortho-phthalaldehyde was used for post column derivatization [31]. Moreover, liquid chromatography tandem mass spectrometry (LC-MS/MS) was a powerful tool applied for the estimation of ACT in plasma and urine [32,33].…”

Section: Research Articlementioning

confidence: 99%

Spectrofluorimetric Estimation of Some Sulfhydryl–Containing Drugs by Demasking Reaction of the Palladium Chelate of 8-Hydroxyquinoline-5-Sulfonic Acid

Rs¹,

Sf²,

Hewala³

et al. 2017

Pharm Anal Acta

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A simple and sensitive spectrofluorimetric method has been developed for the determination of some selected sulfhydryl-containing drugs namely Acetylcysteine (ACS), Captopril (CAP) and Mesna (MSN).The method is based on the interaction of the drugs with potassium (5-sulfoxino) palladium II in alkaline medium in presence of magnesium ions, where the sulfhydryl group combines with palladium from the non-fluorescent potassium bis (5-sulfoxino) palladium II. The resulting 8-hydroxy-5-quinoline sulfonic acid coordinates with magnesium to form the fluorescent chelate that is a measure of the amount of sulfhydrl containing drug analyzed. The fluorescence intensity was measured at an emission wavelength of 485 nm, by excitation at 345 nm. All the experimental parameters affecting the reaction were studied and optimized. The proposed method was applicable over the concentration range of 0.04-0.44 µg/mL for the three drugs and was applied for their determination in bulk form and in pharmaceutical preparations without interference from common excipients. The assay results were statistically compared with those obtained from previously reported methods where no significant difference was found between them. The selectivity and the stability-indicating aspect of the proposed method were confirmed by preparing the disulphides of the studied drugs and applying the reaction to the parent drugs in presence of their disulphides where no interference was detected from these related substances. By virtue of its high sensitivity, the proposed method was also extended to analyze the drugs in spiked human plasma and urine.

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Section: Research Articlementioning

confidence: 99%

Spectrofluorimetric Estimation of Some Sulfhydryl–Containing Drugs by Demasking Reaction of the Palladium Chelate of 8-Hydroxyquinoline-5-Sulfonic Acid

Rs¹,

Sf²,

Hewala³

et al. 2017

Pharm Anal Acta

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“…The reported analytical methods for N-Acetyl cysteine include colorimetric [2], spectrophotometric [3], Chemiluminescence [4], HPLC with electrochemical [5] [6] [7], fluorimetric detection [8] [9] [10], mass [11] [12], ultraviolet detection [13] [14] [15] and Gas chromatographic [16].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of N-Acetyl Cysteine and Taurine by HPTLC Method in Active Pharmaceutical Ingredient and Pharmaceutical Dosage Form

Magesh¹,

Dhanaraju²

2017

AJAC

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Specific, precise and sensitive TLC-Densitometric method was developed and validated for the simultaneous estimation of N-Acetyl cysteine and Taurine in active pharmaceutical ingredient and pharmaceutical dosage form. An effective separation was achieved on pre-coated silica gel HPTLC plates by using n-butanol:acetic acid:water (8:0.5:1.5 v/v/v). The spots were scanned densitometrically at 295 nm. The RF values of N-Acetyl cysteine and Taurine were found to be 0.29 and 0.52, respectively. Calibration curves were linear in the range of 30 -180 and 100 -600 ng/band for N-Acetyl cysteine and Taurine, correspondingly with correlation coefficients of 0.999. The developed method was validated as per ICH guidelines. The limits of detection were 11.24 and 63.40 ng/spot for NAC and TAU respectively. The method developed was found to be precise and specific for the simultaneous analysis of N-Acetyl cysteine and Taurine in pure and tablet dosage form.

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“…[19][20][21][22][23][24][25] Various other analytical methods have also been applied to detect APAP and its metabolites, such as high performance liquid chromatography (HPLC) in combination with accelerator mass spectrometry (AMS), [26] diode-array detection (DAD), [8] chemical reaction interface for mass spectrometry (CRIMS), [27] UV, [9] as well as capillary electrophoresis (CE) with UV detection. Several LC-MS methods have been reported for quantification of APAP [29] or APAP in combination with other analytes, for example other therapeutic drugs, [30][31][32][33] or several metabolites in various matrices. Several LC-MS methods have been reported for quantification of APAP [29] or APAP in combination with other analytes, for example other therapeutic drugs, [30][31][32][33] or several metabolites in various matrices.…”

Section: Introductionmentioning

confidence: 99%

“…[33,40] Phenacetin and p-phenetidine have been included in LC-MS assays for APAP and of its metabolites. [33,40] Phenacetin and p-phenetidine have been included in LC-MS assays for APAP and of its metabolites.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of acetaminophen and structurally related compounds in human serum and plasma

Bylda

Thiele

Kobold

et al. 2013

Drug Testing and Analysis

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The method described in this study allows the simultaneous quantification of acetaminophen (APAP) and nine structurally related compounds, namely acetaminophen metabolites and structurally similar analogs (acetaminophen-glucuronide [APG], -sulfate [APS], mercapturate [APM], -cysteine [APC], p-phenetidine, phenacetin), antidote (N-acetylcysteine, NAC), and two tricyclic antidepressants (imipramine and amitryptiline). Due to the relatively high serum concentration levels in the µg/ml range, matrix effects were simply minimized by dilution. The samples were diluted with water and disulfide bonds between serum proteins and analytes reduced using tris(2-carboxyethyl)phosphine. Chromatographic separation of the analytes was achieved by gradient elution using a pentafluorphenyl (PFP) column with subsequent detection by electrospray ionization (ESI) triple quadrupole mass spectrometry in positive and negative ionization multiple reaction monitoring (MRM) modes. Quantification was performed by means of deuterated analogues of the analytes as internal standards. Total run time of the assay was 19 min. The method was fully validated and allowed quantification of the analytes with lower limits of quantification between 50 and 0.5 ng/ml. The calibration curves were linear over the range 0.1-100 µg/ml for APAP, APG, NAC, p-phenetidine and phenacetin, 0.03-50 µg/ml for APS, and 0.01-10 µg/ml for APM, APC, imipramine and amitriptyline with correlation coefficients r(2) > 0.99. The intra-assay precision was ≤5% for all analytes except NAC (CV < 10%). The inter-day precision was ≤10% for all analytes except NAC (inter-assay precision <11%). This method was used to analyze 77 patient and spiked samples and results were consistent with expected values from a round robin test.

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Determination of N-acetylcysteine in human plasma by liquid chromatography coupled to tandem mass spectrometry

Cited by 30 publications

References 11 publications

Spectrofluorimetric Estimation of Some Sulfhydryl–Containing Drugs by Demasking Reaction of the Palladium Chelate of 8-Hydroxyquinoline-5-Sulfonic Acid

Spectrofluorimetric Estimation of Some Sulfhydryl–Containing Drugs by Demasking Reaction of the Palladium Chelate of 8-Hydroxyquinoline-5-Sulfonic Acid

Simultaneous Determination of N-Acetyl Cysteine and Taurine by HPTLC Method in Active Pharmaceutical Ingredient and Pharmaceutical Dosage Form

Simultaneous quantification of acetaminophen and structurally related compounds in human serum and plasma

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