Determination of myoglobin: comparative evaluation of the new automated VIDASR assay with two other immunoassays

Moigne, F. Le; Beauvieux, M.-C.; Derache, P.; Darmon, Y.M.

doi:10.1016/s0009-9120(02)00306-5

Cited by 7 publications

(7 citation statements)

References 11 publications

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“…[10][11][12] There are several commercial approaches to measuring myoglobin in blood, in addition to new strategies that are still in the demonstration phase. [13][14][15][16][17] The most sensitive commercial assay is the two-site enzyme immunoassay marketed by Dade Behring Inc. 10,12,18,19 This immunoassay requires a laboratorysized Stratus Ò CS Stat Fluorimetric Analyser. An evaluation of this system for myoglobin requires a minimum of 3.0 mL of whole blood ($1.7 mL serum) with an analysis time of 2 h. 19 The detection limit of this assay is reported as 56 pM (1 mg/L) (2 SD over zero measurement) with a linear range of 56 pM-50 nM (1.0-900 mg/L).…”

Section: Introductionmentioning

confidence: 99%

“…Le Moigne et al examined the VIDAS system from bioMerieux, based on an enzyme-linked fluorescent immunoassay method, and suggested that this system had equivalent performance to other commercial systems (detection limit 5 ng/ mL, dynamic range 100 ng/mL, precision <5%). 17 Newer academic approaches include a colloidal gold electrochemical system that has not yet demonstrated equivalent performance to commercial systems, although it holds some promise. 16 Other systems rely on improved luminosity from local field effects, and are also relatively unproven -yet holding promise once developed further.…”

Section: Introductionmentioning

confidence: 99%

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Demonstration of sandwich and competitive modulated supraparticle fluoroimmunoassay applied to cardiac proteinbiomarkermyoglobin

Hayes

Petkus

García

et al. 2009

Analyst

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Modulated supraparticle structures are used to improve sandwich and competitive fluoroimmunoassays. The improved methods are demonstrated on myoglobin, a key diagnostic protein for detection of heart damage. The resulting method uses microliter volumes with bovine serum samples doped with varying concentrations of equine myoglobin. These immunoassays use micron-diameter iron oxide particles as a solid phase for antibody anchoring. Introduction of a magnetic field creates dipole moments on the particles, which attracts them to each other to form rod-like supraparticle structures. These structures can rotate within an alternating magnetic field generating convective flow and a periodic signal that can be analyzed with lock-in amplification enabling more sensitive detection. The system is demonstrated on a target associated with acute myocardial infarction (AMI). This disease causes decreased oxygen delivery to the heart resulting in tissue death and the release of cardiac myoglobin into the bloodstream. Studies have shown that the assessment and monitoring of serum myoglobin concentrations is important when making an early diagnosis of AMI. Early diagnosis is crucial since treatment is most effective when done within the first two hours of symptoms. The modulated assay is rapid, accurate, and sensitive for myoglobin assessment of small-volume serum samples. Using a cut-off value of 5.0 nM (85 ng/mL) for AMI induced myoglobin, the modulated competitive assay was able to diagnose AMI-like conditions in serum doped with myoglobin after an incubation time of only 10 min. The standard curve developed for the modulated sandwich assay was linear over a range of zero to 1 nM (17 ng/mL) with a lower limit of detection at 50 pM (0.85 ng/mL).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Demonstration of sandwich and competitive modulated supraparticle fluoroimmunoassay applied to cardiac proteinbiomarkermyoglobin

Hayes

Petkus

García

et al. 2009

Analyst

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“…Acute myocardial infarction (AMI) disrupts cardiac cell membranes, releasing intracellular cardiac proteins into the vascular system. Some of these proteins, including myoglobin, creatine kinase (CK)-MB, lactate dehydrogenase (LDH) type 1, and cardiac troponin subunits T and I, have proven useful for diagnosing AMI (1). In many patients with chest pain, the correct diagnosis of myocardial cell injury depends mainly on cardiac markers because the electrocardiogram is often nondiagnostic (2).…”

Section: Introductionmentioning

confidence: 99%

Lack of Association Between Cardiac Troponin T and D-Dimer in the Evaluation of Myocardial Damage

Moresco

Vargas

Júnior

et al. 2005

J. Clin. Lab. Anal.

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Acute myocardial infarction (AMI) disrupts cardiac cell membranes, releasing intracellular cardiac proteins into the vascular system. Some of these proteins, including the cardiac troponin subunits T and I, have proven useful for diagnosing myocardial damage. Intracoronary thrombosis plays a key role in the pathogenesis of AMI, and the formation of an occlusive thrombus usually precedes the development of myocardial damage. To evaluate whether there is an association between the size of intracoronary thrombosis and myocardial damage, we analyzed D-dimer and cTnT levels in blood samples from patients suspected to have myocardial damage. We investigated 102 patients who were admitted to emergency service for suspected myocardial damage. D-dimer was assessed with the use of the immunoassay Liatest D-dimer, and cTnT levels were measured with an electrochemiluminescence immunoassay (Troponin T STAT). D-dimer levels were lower in patients with cTnT < 0.01 than in patients presenting cTnT > 0.01 ng/mL. We investigated the relationship between D-dimer and cTnT levels in the patients with cTnT > 0.01 ng/mL (0.40 +/- 0.10 ng/mL), and no significant agreement (r = 0.20, P > 0.05) was observed. The levels of D-dimer were not associated with the levels of cTnT in patients with cTnT > 0.01 ng/mL.

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“…A hemostasia garante o funcionamento adequado do organismo humano pelo equilíbrio entre a manutenção do sangue no estado fluído, permitindo que ele flua pela rede circulatória, e a prevenção de perda sanguínea, que ocorre em situações de risco da integridade vascular. Para a normalidade dessas funções é necessário um potente mecanismo pró-coagulante, para a formação de tampões hemostáticos nos locais comprometidos por lesão vascular e um sistema regulatório capaz de limitar a formação de tampões hemostáticos somente para as áreas comprometidas (LE MOIGNE et al, 2002).…”

Section: Sepse E Sistema Hemostáticounclassified

“…Distúrbios ocorridos no equilíbrio entre os sistemas pró-coagulantes e anticoagulantes devido a fatores genéticos ou adquiridos podem causar alterações hemorrágicas ou trombóticas (LE MOIGNE et al, 2002). No estágio inicial do quadro de sepse, células endoteliais e células mononucleares ativadas produzem citocinas próinflamatórias que ativam os fatores mediadores da coagulação.…”

Section: Sepse E Sistema Hemostáticounclassified

Relação entre parâmetros hemostáticos, evolução clínica e desfecho de pacientes diagnosticados com sepse

Fernandes¹

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simplicidade, sempre me incentivou, estimulou e apoio, tanto nos momentos de vitória como nos momentos de dificuldade, com muito carinho me direcionou nos caminhos em todos os momentos da vida. Esta dissertação é fruto de um aperfeiçoamento pessoal, profissional, acadêmico e também de apoio de várias pessoas Agradecimentos À Professora Angelita, que desde o início desta trajetória, me aceitou como discente, acreditando no meu trabalho, me apoiando e ajudando em todos os momentos, com paciência e sabedoria, desde o início quando tudo não passava de uma ideia. Não somente a construção inicial, não somente do projeto em si, mas também a construção de um profissional acadêmico. Por todo o incentivo, trocas de experiências, demonstrando-se também uma amiga. Meu eterno agradecimento, admiração e respeito. Ao Centro Inter Escolar do Hospital das Clínicas de Ribeirão Preto, que foi onde se iniciou minha carreira dentro da enfermagem, com profissionais tão solícitos, em especial a enfermeira Wanda Vasco Arena da Costa, que foi tão disponível, um exemplo de profissional a se seguir.

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Determination of myoglobin: comparative evaluation of the new automated VIDASR assay with two other immunoassays

Cited by 7 publications

References 11 publications

Demonstration of sandwich and competitive modulated supraparticle fluoroimmunoassay applied to cardiac proteinbiomarkermyoglobin

Demonstration of sandwich and competitive modulated supraparticle fluoroimmunoassay applied to cardiac proteinbiomarkermyoglobin

Lack of Association Between Cardiac Troponin T and D-Dimer in the Evaluation of Myocardial Damage

Relação entre parâmetros hemostáticos, evolução clínica e desfecho de pacientes diagnosticados com sepse

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