Determination of Mucin in Salivary Glands Using Sialic Acids as the Marker by High-Performance Liquid Chromatography with Fluorometric Detection

Comparative studies of HPLC-fluorometry and LC/MS method for the determination of N-acetylneuraminic acid as a marker of deteriorated ophthalmic solutions

“…NeuAc contents of other sialoglycoproteins are also summarized in Table 1. As shown from these data, the concentrations obtained by HPLC-fluorometry in this work shows good accordance with the values from the previous reported data [15,16].…”

The protease-activated receptor-2 agonist induces gastric mucus secretion and mucosal cytoprotection

“…One was essentially the same method as described above for the gastrointestinal mucus (23). The other method was based on the amount of sialic acids that are abundant in salivary, but not gastric, mucin molecules (24). Briefly, the collected saliva was hydrolyzed with 0.1 N HCl, and the amount of N-acetylneuraminic acid was determined by reversephase HPLC after derivatization with 1,2-diamino-4,5-methylenedioxy-benzene.…”

Quantifying the Efficiency ofN-Phenyl-D-mannosamine to Metabolically Engineer Sialic Acid on Cancer Cell Surface

“…Except for LC-MS, sialic acids must be derivatized to be photometrically detectable. In addition to thiobarbituric acid, 14,15 a number of reagents including 3,4-diaminotolenene, 32 1,2-diamino-4,5-dimethoxybenzene, 17 O -phenylenediamine, 20 and 1,2-diamino-4,5-methylenedioxybenzene (DMB), 21–29,33,34 have been used for sialic acid derivatization, taking advantage of the special α-keto acid functionality. Among these methods, DMB derivatization for HPLC-fluorescence and LC-MS analyses was the most popular due to the high sensitivity and selectivity of the reaction involved.…”