Determination of monoureides in biological fluids by high-pressure-liquid-chromatography

Abstract

A sensitive and specific method for the simultaneous determination of bromisoval, carbromal, and methaqualone is described. The drugs are adsorbed from serum onto charcoal at pH 11 and eluted from it with organic solvent. The eluate is separated by high-pressure liquid-chromatography on reverse phase (RP 18) column using acetonitrile: water (26 : 74 by volume) as mobile phase. The eluted drugs are detected by uv-absorption at 210 nm. The method is sensitive, specific, precise, and accurate.

Precolumns of Amberlite XAD-2 for direct injection liquid chromatographic determination of methaqualone in blood plasma

“…Among the standard techniques used for concentration and cleanup when dealing with biological samples are solvent extraction and adsorption onto columns of the nonpolar adsorbent Amberlite XAD-2. Both of these techniques have been used to prepare blood plasma samples for gas or liquid chromatographic determination of methaqualone (MTQ), a sedative, hypnotic drug (1)(2)(3)(4)(5)(6)(7)(8). An alternative to using adsorbent columns "off-stream" is the "on-stream" incorporation of them into the flow scheme of a liquid chromatograph as a "precolumn" in advance of the anaytical column.…”