Determination of mold contamination using ergosterol imprinted particles

Abstract

Ergosterol is a key biochemical marker for fungal mycelial growth. In this study, molecularly ergosterol imprinted particles (Erg-MIPs) were newly synthesized for the selective detection of ergosterol in mold samples. Erg-MIPs were characterized via scanning electron microscopy, swelling studies, and surface area measurements. Maximum selective ergosterol adsorption achieved as 28.50 mg/g Erg-MIP. Selectivity studies showed that Erg-MIPs adsorbed Erg 2.01 and 3.27 times higher than that of cholesterol and stigmasterol, respectively. Erg adsorption from Aspergillus niger was found as 23.87 mg/g. Reusability of Erg-MIPs was studied and decrease in Erg adsorption capacity of the particles was negligible (3%). Erg-MIPs are good affinity materials for the selective Erg detection from food samples, prior to use in food industry.

Antimicrobial activity of oridonin

“…The polysaccharide content in the mycelium solution increases, and the content of total polysaccharides in bacteria decreases. The decrease in ergosterol level shows that the R. rubescens extract has an inhibitory effect on the synthesis of the intracellular substances of fungi (Oktay Başeğmez et al, 2021). The antifungal effect of R. rubescens extract on Ascochyta molleriana wint is achieved by damaging the cell membrane and inhibiting the synthesis of protein, which is consistent with the antibacterial mechanism clarified by other natural antibacterial agents and has a guiding significance for the antifungal research of oridonin in R. rubescens (Ju et al, 2020;Meng et al, 2020;Yin et al, 2021).…”

Synthesis of PHEMA-Based Polymeric Particles for Gallic Acid Purification

“…Elde edilen monolitik kolon öğütüldü ve partiküller elekten geçirilerek 1 µm boyutun altındaki partiküller adsorpsiyon çalışmalarında kullanılmak üzere seçildi (AS200, Retsch Standard sieves, Retsch, Haan, Gemany). Üretilen partiküller, reaksiyona katılmayan monomerlerin ve safsızlıkların uzaklaştırılması için oda sıcaklığında etanol/saf su (30/70, v/v) çözeltisi ile yıkandı [16].…”

“…Kuru ağırlığı belirlenen partiküller ultra saf su içerisinde oda sıcaklığında dengeye ulaşana kadar bekletildi ve partiküllerin şişmiş ağırlığı belirlendi. Partiküllerin şişme oranı eşitlik (1) kullanılarak hesaplandı: Şişme Oranı (%) = [(Ws -Wo)/Wo] x %100 [16,17] (…”