Determination of mofebutazone and its 4-hydroxy metabolite in plasma and urine by high-performance liquid chromatography

Delbeke, Frans; Debackere, M.

doi:10.1016/s0021-9673(00)90155-9

Cited by 4 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentrations of SXB and its metabolites in plasma and urine were measured by modification of the HPLC method for mofebutazone and metabolites (Delbeke & Debackere, 1986a). Plasma samples were prepared in duplicate by pipetting 2.0 ml into a 15 ml screw-capped tube, followed by the addition of 50 pI of internal standard solution (diclofenac in methanol, 100 pg/ml), 250 p1 1 M HCl and 5 ml diethylether.…”

Section: Determination Of Plasma and Urinary Concentrations Of Suxibumentioning

confidence: 99%

The disposition of suxibuzone in the horse

Delbeke

Vynckier

Debackere

1993

Vet Pharm & Therapeutics

Self Cite

View full text Add to dashboard Cite

A high performance liquid chromatographic method is described to determine the anti-inflammatory drug suxibuzone (SXB) and its major metabolites phenylbutazone (PBZ) and oxyphenbutazone (OPBZ) in equine plasma and urine. When suxibuzone (6 mg/kg) was administered intravenously (i.v.) or orally (p.o.) no parent drug was detected in plasma or in urine. The disposition of the metabolite PBZ (i.v.) could be described by a 2 compartment model with a beta half-life varying from 7.40 to 8.35 h. Due to severe side effects the use of i.v. suxibuzone should not be encouraged in the horse. PBZ and OPBZ were detected in plasma and urine after p.o. SXB administration. Peak plasma PBZ concentrations (8.8 +/- 3.0 micrograms/ml) occurred 6 h after oral dosing and the terminal exponential constant was 0.11 +/- 0.01 h-1. Phenylbutazone and oxyphenbutazone were detectable in urine (> 1 microgram/ml) for at least 36 h, after p.o. administration. SXB was not hydrolyzed in vitro by horse plasma. Equine liver homogenates however appeared to have a very high capacity for hydrolysing SXB, indicating that first-pass effect could be responsible for the rapid disappearance of this NSAID in the horse.

show abstract

Section: Determination Of Plasma and Urinary Concentrations Of Suxibumentioning

confidence: 99%