Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method

Owuama, C.I.

doi:10.5897/ajmr2017.8545

Cited by 118 publications

(56 citation statements)

References 5 publications

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“…The inhibition rate was evaluated by measuring the distance between the hole and the nearest growth area in mm. The minimal inhibitory concentration (MIC) of the extract was calculated using serial dilution concentrations (50, 25, 12.5, 6.25, and 3.13 mg ⁄ml) as described before [47].…”

Section: The Antibacterial Activity Evaluationmentioning

confidence: 99%

Antimicrobial Potential of Biosynthesized Silver Nanoparticles by Aaronsohnia factorovskyi Extract

et al. 2020

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The green biosynthesis of nanoparticles by plant extracts is an attractive and promising technique for medicinal applications. In the current study, we chose one of the daisy plants, Aaronsohnia factorovskyi (which grows in the Najd region, Saudi Arabia), to investigate its anti-microbial efficacy, in combination with silver nanoparticles. The biosynthesized nanoparticles were evaluated for antibacterial activity against Staphylococcus aureus, Bacillussubtilis (Gram-positive), Pseudomonas aeruginosa, and Escherichia coli, (Gram-negative) using the disc diffusion method, while the antifungal activity was assessed against Fusarium oxysporum, Fusarium solani, Helminthosporiumrostratum, and Alternariaalternata. The potential phytoconstituents of the plant extracts were identified by Fourier-transform infrared spectroscopy (FT-IR) techniques, the Field emission scanning electron microscopy (FE-SEM), Chromatography/Mass Spectrometry (GC-MS) techniques, and Zeta potential analysis. The current study revealed the ability of the tested plant extract to convert silver ions to silver nanoparticles with an average diameter of 104–140 nm. Biogenic Aaronsohnia factorovskyi-silver nanoparticles (AF-AgNPs) showed significant antibacterial activity against Staphylococcus aureus with inhibition zone diameter to 19.00 ± 2.94 mm, and antifungal activity against Fusarium solani, which reduced the growth of fungal yarn to 1.5 mm. The innovation of the present study is that the green synthesis of NPs, which is simple, cost-effective, provides stable nano-materials, and can be an alternative for the large-scale synthesis of silver nanoparticles.

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Section: The Antibacterial Activity Evaluationmentioning

confidence: 99%

Antimicrobial Potential of Biosynthesized Silver Nanoparticles by Aaronsohnia factorovskyi Extract

et al. 2020

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“…To determine the minimum inhibitory concentrations (MIC) of plant extracts, 100 µL of Mueller Hinton Broth medium (Merk, Germany) was initially applied to each of a microtiter plate's wells (24,25). Then 100 µL of 20 mg/mL extract was added to the first well, and after mixing, 100 µL of the extract was removed from the first well and added to the second well.…”

Section: Determination Of the Mic And Mbc Of Plant Extracts Against Escherichia Colimentioning

confidence: 99%

“…To determine the minimum bactericidal concentration (MBC), 10 µL of the wells' content was applied to the nutrient agar medium (Merk-Germany) at the end of the 24-hour incubation period. The lowest plant extract's concentration at which 99.9% of bacteria did not grow was considered as MBC (24,25). All antimicrobial tests were repeated three times.…”

Section: Determination Of the Mic And Mbc Of Plant Extracts Against Escherichia Colimentioning

confidence: 99%

The Effects of Different Solvents on the Growth-inhibitory Activity of Rhazya stricta Extract Against Antibiotic-Resistant Escherichia coli

2021

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Background: Escherichia coli (Gram-negative bacilli) inflicts large economic losses on the poultry industry and is one of the most important causes of poultry diseases. The indiscriminate use of antibiotics has contributed to today’s increasing prevalence of drug-resistant strains, which their emergence appears to exceed the discovery of new drugs. Therefore, several attempts have been dedicated to find new compounds as effective alternatives to antibiotics. Medicinal plants constitute a rich source for various antimicrobial compounds. Objectives: The aim of this study was to evaluate the antibiotic resistance trend of the E. coli strains isolated from Quail feces samples and to investigate the antimicrobial effects of Eshvarak extract against these strains. Methods: Eshvarak plant was collected from Saravan (Sistan and Baluchestan province, Iran) and identified in the botany laboratory of Zabol University. E. coli samples were isolated from poultry feces. Various solvents (methanol (100%), ethanol (100%), water (100%), hydro-alcohol (70%), and ethyl-acetate (100%)) were used to prepare Eshvarak extract. Inhibitory zone diameter was determined in an agar-based medium using a standard procedure. The MIC and MBC of prepared extracts were determined by the micro-dilution method. Results: The lowest MIC values were obtained for the methanolic (12.5 ppm), ethanolic (12.5 ppm), aqueous (12.5 ppm), hydroalcoholic (25 ppm), and ethyl-acetate (12.5 ppm) Eshvarak extracts. The highest inhibitory zone diameters against E. coli were recorded at the 100-ppm concentration of the methanolic (8 mm), ethanolic (7 mm), aqueous (8 mm), hydroalcoholic (10 mm), and ethyl-acetate (5 mm) Eshvarak extracts. Conclusions: Eshvarak extract, particularly in the hydroalcoholic solvent, inhibited the growth of E. coli. However, the antimicrobial properties of plant extracts seem to be independent of the extraction method or the type of solvent.

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“…Also, no growth was determined in the negative control tube whereas growth was determined in the positive control tube. The MIC value was determined by taking half of the sum of the concentrations of the first tube evaluated as positive growth and the tubes previously evaluated as negative growth (By Aamer et al, 2015;Chikezie, 2017;Akarca, 2019).…”

Section: Minimum Inhibitory Concentration (Mic) and Minimum Bactericimentioning

confidence: 99%

The Antibacterial Effects of the Different Extracts of Oenothera biennis and Origanum minutiflorum O. Schwarz et. P. H. Davis on Food-borne Pathogenic Bacteria

Akarca

Tomar

2020

SJAFS

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Oenothera biennis is a biennial plant from the Convolvulaceae family. It is commonly known as evening primrose. The plant grows well in sandy, loamy and clayey soils in almost every place such as fields, roadsides and meadows (Morrison and Reekie, 1995). The oil obtained from the seeds of the plant was determined to have various pharmacological properties (Arimura, 2003a). The seed oil of the plant is very rich in γlinolenic acid content, an important fatty acid found in the composition of prostaglandins and related hormones that are effective in regulating muscle and vascular contractions in human physiology. (Becker, 1983; Shukla et al., 1999). Also, recent studies have shown that ethanol extract obtained from the seeds of the plant shows specific anti-tumor activity (Arimura et al., 2003b; Arimura et al., 2004).

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Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method

Cited by 118 publications

References 5 publications

Antimicrobial Potential of Biosynthesized Silver Nanoparticles by Aaronsohnia factorovskyi Extract

Antimicrobial Potential of Biosynthesized Silver Nanoparticles by Aaronsohnia factorovskyi Extract

The Effects of Different Solvents on the Growth-inhibitory Activity of Rhazya stricta Extract Against Antibiotic-Resistant Escherichia coli

The Antibacterial Effects of the Different Extracts of Oenothera biennis and Origanum minutiflorum O. Schwarz et. P. H. Davis on Food-borne Pathogenic Bacteria

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