Determination of minimum inhibitory and minimum bactericidal concentrations of Brazilian strains of <i>Leptospira</i> spp. for streptomycin sulphate

Rocha, Bruno Ribeiro; Martins, Gabriel; Cruz, Breno Cayeiro; Lilenbaum, Walter

doi:10.1017/s0950268820002228

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“…Curcusionol, the most active compound, presented the similar potent bactericidal activity against R . nicotianae to streptomycin sulfate, regarded as effective for many bacteria 51 . The antibacterial activities of curcusionol and the other nine compounds extracted from Carex siderosticta Hance against R .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity and action mechanism of curcusionol from Carex siderosticta Hance against Ralstonia nicotianae

Ren,

Zhou,

Liu

et al. 2023

Pest Management Science

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BACKGROUNDTobacco bacterial wilt is a typical soil‐borne disease caused by Ralstonia nicotianae, which causes huge losses in tobacco production every year. The crude extract of Carex siderosticta Hance was shown to have antibacterial activity against R. nicotianae during our search, and the natural antibacterial components were sought after using bioassay‐guided fractionation of the compounds.RESULTEthanol extract of Carex siderosticta Hance with the minimum inhibitory concentration (MIC) value of 100 μg/mL against R. nicotianae in vitro. The potential of these compounds as antibactericides against R. nicotianae were assessed. Curcusionol (1), showed the highest antibacterial activity against R. nicotianae with MIC value of 12.5 μg/mL in vitro. In the protective effect tests, the control effect of curcusionol (1) was 92.31 and 72.60%, respectively, after application of 7 and 14 days, at a concentration of 1500 μg/mL, being comparable to that of streptomycin sulfate at a concentration of 500 μg/mL, confirming that curcusionol (1) showed the potential for the development of new antibacterial drugs. RNA‐sequencing, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis confirmed that curcusionol mainly destroys R. nicotianae cell membrane structure and affects quorum sensing (QS) to inhibit pathogenic bacteria.CONCLUSIONThis study revealed that the antibacterial activity of Carex siderosticta Hance makes it a botanical bactericide against R. nicotianae, while curcusionol as lead structures for antibacterial development is obvious by its potent antibacterial activity. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%