Determination of microRNAs in mouse preimplantation embryos by microarray

Yang, Yanhong; Bai, Wentao; Zhang, Liang; Guo, Yijia; Wang, Xiaohong; Wang, Jun; Zhao, Hongxi; Han, Yang; Yao, Yuanqing

doi:10.1002/dvdy.21666

Cited by 69 publications

(74 citation statements)

References 60 publications

(65 reference statements)

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“…Although it has been reported that miRNAs undergo dynamic changes during preimplantation embryo development [41], the exact nature of the environmental cues that lead to such alterations in embryos and the underlying mechanism have not been elucidated. The present study has demonstrated for the first time that extracellular HCO 3 − can regulate embryonic miR-125b through CFTR-mediated entry and subsequent sAC, PKA and NF-κB activation, which is important for preimplantation embryo development ( Figure 6).…”

Section: Discussionmentioning

confidence: 99%

CFTR mediates bicarbonate-dependent activation of miR-125b in preimplantation embryo development

et al. 2012

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Section: Discussionmentioning

confidence: 99%

CFTR mediates bicarbonate-dependent activation of miR-125b in preimplantation embryo development

et al. 2012

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“…[27][28][29] After the first cell division, the pool of maternal miRNAs is significantly downregulated, with some miRNAs selectively reduced by 95%. Subsequently, upon activation of the zygotic transcription, the number of expressed miRNAs is increased.…”

Section: Zebrafishmentioning

confidence: 99%

MicroRNAs in early vertebrate development

Rosa

Brivanlou

2009

Cell Cycle

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“…The dynamic changes in the expression of miRNAs in preimplantation embryos (6)(7)(8) and the increased synthesis of miRNAs after the two-cell stage in mouse embryos (7,9) suggest that miRNAs have a functional role in the preimplantation period. This evidence is supported by the observations that mouse oocytes without the miRNA-processing enzyme Dicer have a minimal amount of miRNA, that their resulting zygotes cannot pass through the first cleavage division (7), and that Dicer-null embryos carrying maternal dicer die at embryonic day 7.5 (E7.5) (10).…”

mentioning

confidence: 99%

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

Liu

Pang

Chiu

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

374

332

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In mammals, the sperm deliver mRNA of unknown function into the oocytes during fertilization. The role of sperm microRNAs (miRNAs) in preimplantation development is unknown. miRNA profiling identified six miRNAs expressed in the sperm and the zygotes but not in the oocytes or preimplantation embryos. Sperm contained both the precursor and the mature form of one of these miRNAs, miR-34c. The absence of an increased level of miR-34c in zygotes derived from α-amanitin-treated oocytes and in parthenogenetic oocytes supported a sperm origin of zygotic miR-34c. Injection of miR-34c inhibitor into zygotes inhibited DNA synthesis and significantly suppressed first cleavage division. A 3′ UTR luciferase assay and Western blotting demonstrated that miR-34c regulates B-cell leukemia/lymphoma 2 (Bcl-2) expression in the zygotes. Coinjection of anti-Bcl-2 antibody in zygotes partially reversed but injection of Bcl-2 protein mimicked the effect of miR-34c inhibition. Oocyte activation is essential for the miR-34c action in zygotes, as demonstrated by a decrease in 3′ UTR luciferase reporter activity and Bcl-2 expression after injection of precursor miR-34c into parthenogenetic oocytes. Our findings provide evidence that sperm-borne miR-34c is important for the first cell division via modulation of Bcl-2 expression.

show abstract

Determination of microRNAs in mouse preimplantation embryos by microarray

Cited by 69 publications

References 60 publications

CFTR mediates bicarbonate-dependent activation of miR-125b in preimplantation embryo development

CFTR mediates bicarbonate-dependent activation of miR-125b in preimplantation embryo development

MicroRNAs in early vertebrate development

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

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