Determination of Microcytic Hypochromic Anemia (MCHC) for Hemoglobin Variants and Hemoglobinopathies by Using High Performance Liquid Chromatography (HPLC)

Kharche, Kaustubh Vilasrao; Bhake, Arvind

doi:10.9734/bpi/capr/v4/2290b

Cited by 2 publications

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“…Anemia in which both MCV and MCHC are low are called microcytic, hypochromic anemia. While when both MCV and MCHC are high is called macrocytic Anemia [24,25,26].…”

Section: Discussionmentioning

confidence: 99%

Macrocytic anemia induced via oral administration of toxic dose of acetaminophen

Okerulu¹,

Onwuka²

2022

GSC Biol. and Pharm. Sci.

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The abuse of Acetaminophen (over-the-counter drug) is rapidly increasing globally, thus inducing physiological alterations and toxicity. In this study, it was hypothesized that overdose of acetaminophen may induce certain type of anemia; hence the type of anemia induced by oral exposure to toxic dose of acetaminophen was evaluated. Fifteen (15) Wistar rats weighing approximately 160 g were grouped into three (3) groups; Group 1 (control) received 2ml of distilled water, Group 2 received 200 mg/kg of acetaminophen and Group 3 received 700 mg/kg of acetaminophen for 14 days. After administration, the rats were dissected on anesthesia (chloroform); blood was collected via cardiac puncture. The samples collected were assayed for Red Blood cell (RBC) Count, Hemoglobin concentration (HB), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC). The data was statistically analyzed using Graph Pad Prism (version 8). Statistical significance was considered at P < 0.05. Results showed that acetaminophen (200 mg/kg and 700 mg/kg) significantly increased MCV, MCH and decreased RBC and HB in a dose dependent manner with no significant effect on MCHC when compared to group 1 (control) (P<0.05). In conclusion, Acetaminophen (700 mg/kg) decreases RBC, HB and increases MCV, MCH and MCHC which suggests that toxic dose of acetaminophen can cause macrocytic anemia in Wistar rats.

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“…Anemia in which both MCV and MCHC are low are called microcytic, hypochromic anemia. While when both MCV and MCHC are high is called macrocytic Anemia [24,25,26].…”

Section: Discussionmentioning

confidence: 99%

Macrocytic anemia induced via oral administration of toxic dose of acetaminophen

Okerulu¹,

Onwuka²

2022

GSC Biol. and Pharm. Sci.

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“…As a result, it has become a serious public health issue. 14 Haemolytic anaemia is characterized by excessive destruction of the RBCs in the central and peripheral circulation. The causes behind the excessive RBC destruction are attributed to either the extrinsic or the intrinsic defects that may be present in the RBC.…”

Section: Rationalementioning

confidence: 99%

Assessment and comparison of the sensitivity and specificity of sickling and haemoglobin electrophoresis in haemolytic anaemia patients: A study protocol

Mungale,

Singh Chauhan,

Jagtap

2023

F1000Res

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The present protocol will compare haemoglobin electrophoresis with sickling test, evaluating sensitivity, specificity, positive predicted value and negative predicted value of haemolytic anaemia patients. Haemoglobin electrophoresis is the confirmation test for sickle cell anaemia. Sickle cell anaemia is a type of haemolytic anaemia where red blood cells (RBC)’s biconcave form does not enable the best possible respiratory exchange. In haemolytic anaemia, EDTA is used to examine the peripheral smear stained with Giemsa stain. Peripheral smear shows polychromatic neutrophils, nucleated RBCs (Red blood cell) and fragmented RBCs. In haemolytic anaemia, patients have decreased haemoglobin (Hb) levels. In sickle cell disease (SCD), anaemia is brought about by a change in the DNA sequence of cells in the Hb beta-globin chain. Screening of the sickling test is done by using sodium metabisulfite to assess the shape of cells. Confirmation test of Hb electrophoresis on cellulose acetate paper uses band detection. This study will aim to evaluate the sensitivity and specificity of Hb electrophoresis with the sickling test utilizing quantifiable investigation. The effectiveness of sensitivity and specificity, positive and negative predicted values, and overall course of action for the two techniques, Hb electrophoresis and sickling test, will be collected. This research will also give an overview of existing haemolytic anaemia literature.

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Determination of Microcytic Hypochromic Anemia (MCHC) for Hemoglobin Variants and Hemoglobinopathies by Using High Performance Liquid Chromatography (HPLC)

Cited by 2 publications

References 0 publications

Macrocytic anemia induced via oral administration of toxic dose of acetaminophen

Macrocytic anemia induced via oral administration of toxic dose of acetaminophen

Assessment and comparison of the sensitivity and specificity of sickling and haemoglobin electrophoresis in haemolytic anaemia patients: A study protocol

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