Determination of microcystins and anatoxin-a using liquid chromato-mass-spectrometry of unit resolution

Chernova, Ekaterina; Russkikh, Y. V.; Podolskaya, Е. P.; Zhakovskaya, Z. А.

doi:10.18358/np-26-1-i1125

Cited by 5 publications

(2 citation statements)

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“…The fragment spectra of the linearized form of MC-LR (Figure 6b) contained a number of characteristic ion products with m/z 995; 862; 571 [45], and the additional ion products presented in the fragment spectrum of MC-LR with m/z 844; 728; 710; 599; 571; 553; 470 [46]. It should be noted that the linearized form of MC-LR in fungi destructors was revealed for the first time.…”

Section: Determination Of Mc-lr Biotransformation Productsmentioning

confidence: 86%

“…The target compounds were identified according to the established retention times (with an error not exceeding 0.3 min), the exact m/z values of [M + H] + and [M + 2H] 2+ ions (the mass determination accuracy was 5 ppm) [55], and the data of complete fragment spectra [46]. The quantitative determination was carried out using the external standard method.…”

Section: Quantification Of Mc-lr and Identification Of Its Transforma...mentioning

confidence: 99%

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Algicidal Activity and Microcystin-LR Destruction by a Novel Strain Penicillium sp. GF3 Isolated from the Gulf of Finland (Baltic Sea)

Kuzikova,

Zaytseva,

Chernova

et al. 2023

Toxins

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The present article focuses on a strain of ascomycete GF3 isolated from a water sample taken in the Gulf of Finland. Based on phylogenetic analysis data, the isolate was identified as Penicillium sp. GF3. The fungus GF3 demonstrates algicidal activity towards cyanobacteria (98–100%). The algicidal effect on green algae did not exceed 50%. The isolate GF3 exhibits an indirect attack mode by releasing metabolites with algicidal and/or lytic activity into the environment. Moreover, the strain Penicillium sp. GF3 is able to degrade MC-LR. After 72 h of GF3 cultivation, the MC-LR content was reduced by 34.1% and 26.7% at initial 0.1 μg/mL and 0.45 μg/mL concentrations, respectively. The high stress resistance of the GF3 to toxic MC-LR is provided by a 1.5-fold activation of catalase activity and a change in the reduced glutathione content. Additionally, during the MC-LR biotransformation, a MC-LR-GSH conjugate and linearized MC-LR were identified. The linearized MC-LR in the presence of fungi capable of degrading MCs was revealed for the first time. Using Daphnia magna as a bioindicator, it was shown that the MC-LR biotransformation led to the formation of less toxic intermediates. The toxicity of the fungal filtrate is reduced by five times compared to the abiotic control. Our findings enhance the understanding of the role that ascomycete fungi have as potential bioagents for cyanoHABs to control and detoxify water bodies.

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Section: Determination Of Mc-lr Biotransformation Productsmentioning

confidence: 86%

Section: Quantification Of Mc-lr and Identification Of Its Transforma...mentioning

confidence: 99%

Algicidal Activity and Microcystin-LR Destruction by a Novel Strain Penicillium sp. GF3 Isolated from the Gulf of Finland (Baltic Sea)

Kuzikova,

Zaytseva,

Chernova

et al. 2023

Toxins

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Microcystin-LR Biodestruction by Autochthonous Microbiota of Different Water Bodies in the Northwest of Russia

Medvedeva,

Zaytseva,

Kuzikova

et al. 2023

Biol Bull Russ Acad Sci

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Mass Spectrometric Analysis of Microcystins from Cyanobacterial Biomass: Optimization of the Sample Preparation Procedure

et al. 2017

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Determination of microcystins and anatoxin-a using liquid chromato-mass-spectrometry of unit resolution

Cited by 5 publications

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Algicidal Activity and Microcystin-LR Destruction by a Novel Strain Penicillium sp. GF3 Isolated from the Gulf of Finland (Baltic Sea)

Algicidal Activity and Microcystin-LR Destruction by a Novel Strain Penicillium sp. GF3 Isolated from the Gulf of Finland (Baltic Sea)

Microcystin-LR Biodestruction by Autochthonous Microbiota of Different Water Bodies in the Northwest of Russia

Mass Spectrometric Analysis of Microcystins from Cyanobacterial Biomass: Optimization of the Sample Preparation Procedure

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