Determination of microbial load, total Phenolic and flavonoids contents in polyherbal formulation “yograj guggulu vati”

Dutt, V. V. S. Eswara; Srivastav, Shivani; Mittal, Swati; Haque, Rafiul

doi:10.22270/jddt.v10i1-s.3858

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…DPPH solution measures the electron-donating ability of the herbal molecules. The activity is based upon the potential of natural products to decolourize the DPPH solution as the change in colour is directly proportional to the concentration of antioxidant [38]. In the present study, it was observed that the DPPH radical scavenging activity was positively correlated to the concentration of the extract.…”

Section: Fluorescence Analysissupporting

confidence: 56%

Pharmacognostical and Phytochemical Evaluation of a Unani Polyherbal Formulation: Dawa ul Kurkum by HPTLC

Gupta

Sumaiya

Ali³

et al. 2023

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Background: Dawa ul Kurkum (Duk) is a widely used Unani formulation. It consists of seven plant herbs, including stigmas of Crocus sativus L., rhizomes of Nardostachys jatamansi (D.Don) DC., the bark of Cinnamomum cassia (L.) J. Presl., shoot of Cymbopogon jwarancusa (Jones ex Roxb.) Schult., the resin of Commiphora wightii (Arn.) Bhandari, roots of Saussurea lappa (Decne.) Sch.Bip., and bark of Cinnamomum zeylanicum Blume. However, no study has been previously conducted to characterize this formulation. Thus, the present study was designed to carry out the pharmacognostic and phytochemical characterization of Duk. Methods: Duk was prepared following the protocols in Bayaz e Kabeer and The National Formulary of Unani Medicine Part-I. The characterization included organoleptic properties, fluorescence analysis, preliminary phytochemical screening, antioxidant activity, and active constituent profiling using HPTLC. Results: Evaluation of Duk showed the presence of carbohydrates, flavonoids, quinones, glycosides, cardiac glycosides, terpenoids, phenols, coumarin, steroids, and phytosterols. The total phenolic and flavonoid content was 5.75 ± 0.23 mg GAE/g and 10 ± 0.18 mg QUE/g, respectively. HPTLC of Duk showed the presence of p-coumaric acid, cinnamaldehyde, citral, crocin, isovaleric acid, guggulsterone, and dehydrocostus lactone. Conclusions: Our findings supported the use of Duk as a conventional medicine, and these results could be used as a reference for the standardization of Duk.

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Section: Fluorescence Analysissupporting

confidence: 56%

Pharmacognostical and Phytochemical Evaluation of a Unani Polyherbal Formulation: Dawa ul Kurkum by HPTLC

Gupta

Sumaiya

Ali³

et al. 2023

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“…Took 0.2 mL extract to determine the total phenol content. The total phenolics content of the extract was determined using the Folin-Ciocalteu method 25 . The standard curve was established as:…”

Section: Total Phenolics Extractionmentioning

confidence: 99%

Studies on Adsorption Kinetics and Thermodynamics of Macroporous Resin for Rosmarinic Acid

Zhang

Bai

et al. 2021

J. Oleo Sci.

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This experiment treated perilla seeds with different concentrations of NaCl solution to enrich and purify their rosmarinic acid (RosA). The results showed that low concentrations of salt (0-20 mmol/L) promoted seed germination, while high concentrations (> 20 mmol/L) inhibited germination. When the salt concentration was 20 mmol/L, the germination rate was the highest. The content of RosA in germinated perilla seeds was 3.5 mg/g, which was 3.5 times as much as that in the seeds without germination. The RosA was purified using NK-109 macroporous resin and its adsorption kinetics, isotherms and thermodynamics were determined. The adsorption kinetics showed that the adsorption behavior of RosA in NK-109 resin conformed to the pseudo-second-order kinetic model. The model for RosA in the NK-109 resin exhibited Langmuir adsorption based on a spontaneous exothermic process according to its adsorption thermodynamics, which included both physical and chemical adsorption. The optimized process conditions were as follows: the loading concentration of 0.04 mg/mL, loading volume of 40 mL, 70% methanol as the eluent with the volume of 60 mL, and the purity of RosA was 42.1%.

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“…Preparation of blank solution: Taken 2 ml of methanol, added 0.1 ml aluminium chlorides and 0.1ml of sodium acetate, & then added 2.8 ml of distilled water. After taking absorbance of standard dilution as mentioned above, draw a calibration curve and finded out the regression equation and calculated the total flavonoid content 7 . The standard curve of quercetin is shown in figure 1.…”

Section: Determination Of Flavonoid Contentsmentioning

confidence: 99%

“…The microbial load was determined as per procedure of Dutt et al 2020 7,9 . The medium was autoclaved at 151lbs per square inch pressure at 121 °C.…”

Section: Determination Of Microbial Counts Explained As Per Whomentioning

confidence: 99%

Total phenolic and flavonoids contents in the standardized polyherbal formulation “vayusadi guggulu”

Vp¹,

Dhaka²,

Dhama³

et al. 2020

J. Drug Delivery Ther.

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Vayusadi guggulu vati (VGV), a polyherbal formulation is recommended for the management of diseases like obesity, arthritis, hyperlipidaemia and hyper cholesterol. Though Vayusadi guggulu vati is widely used for the treatment of obesity in Ayurvedic System of Indian Medicine, but till date, its quality standard study has not been carried out. In the present article, we evaluated the total phenolic and flavonoid contents in the standarised polyherbal VGV. The total phenolic content was determined by Folin Ciocalteu reagent (FC reagent) method. Aluminum chloride colorimetric method was used for total flavonoids contents determination. The VGV was standardized by physic-chemical parameters like total ash value, acid insoluble ash, loss on drying (LOD), PH, extractive value, phytochemical tests and thin layer chromatography (TLC). Determination of microbial load in different dilutions was also performed. Quality determination of vati (tablet) was also evaluated with help of various tablet parameters. Microbial load study revealed the growth of microbes increases with increases the dilutions. The phenolic and flavonoid contents in VGV were 190.16 ± 5.07 mg/g and 80.216 ± 2.07 mg/ g respectively. Physicochemical parameters such as total ash value (9.73 ± 1.45% W/W), acid insoluble ash (1.85± 0.40 % W/W), LOD (4.77 ± 0.45%W/W), PH 1% (4.6) water soluble extract (64.69 ± 3.42% W/W), alcohal soluble extract (50.56 ± 2.48% W/W) were assessed in preliminary physicochemical scanning. Thin layer chromatography (TLC) fingerprints study revealed that alcoholic and hexane extract of formulation showed 3 spots with different resolution in long wave UV 366 nm. TLC study revealed genuinely, quality and purity of formulation. Physico-chemical and microbial load result revealed that the formulation has a good quality. The inference from the present study may be used as reference standard in the further quality control researches. Key words: Phenolic contents, flavonoid contents, microbial load, vayusadi guggulu vati

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Determination of microbial load, total Phenolic and flavonoids contents in polyherbal formulation “yograj guggulu vati”

Cited by 4 publications

References 8 publications

Pharmacognostical and Phytochemical Evaluation of a Unani Polyherbal Formulation: Dawa ul Kurkum by HPTLC

Pharmacognostical and Phytochemical Evaluation of a Unani Polyherbal Formulation: Dawa ul Kurkum by HPTLC

Studies on Adsorption Kinetics and Thermodynamics of Macroporous Resin for Rosmarinic Acid

Total phenolic and flavonoids contents in the standardized polyherbal formulation “vayusadi guggulu”

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