Determination of Mianserin Using Tropaeolin-Ooo by Ion Pair Formation

Abstract

Objective: The present study was aimed at the development of a simple visible spectrophotometric method for the assay of mianserin, a drug used for the treatment of depression.Methods: The method was developed using tropaeolin-ooo (TPooo) as an ion associative complex forming a chromophore. Developed the chromophore by sequential mixing of aqueous solutions of mianserin, hydrochloric acid, and TPooo. Chromophore was extracted into an organic solvent (chloroform) and absorbance values of organic layers were measured. As per the existing guidelines of an international conference on harmonization (ICH), various parameters of the method were tested for validation.Results: At the optimized reaction conditions, the formed chromophore (λ max 524 nm) was stable and sensitive. Regression analysis (r>0.9999) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (4–24 µg/ml). Accuracy of the method was evident from the % recovery values (99.50–99.87 range). Satisfactory precision (both intra and inter day) for the proposed method was clear as ranges of percentage of relative standard deviation (%RSD) values were 1.382-1.781 and 1.128-1.765 respectively. Since RSD is less than 2 %, this method was reproducible and accurate.Conclusion: Due to lack of pre-treatment process for this method, it was simple. All the tested parameters of the method were validated as per ICH guidelines.

Piperacillin Estimation by Ion-Associative Complex Formation

“…Spectrophotometric method is the generally used method and of choice for routine analysis in industrial quality control (QC) laboratories, especially in developing countries [27][28][29][30][31][32][33][34][35][36]. Hence, the present study is an attempt to develop a sensitive visible spectrophotometric method for the determination of PIP in pure and powder for injection formulations using an extractive ion pair complex-forming agent like CTC.…”

Visible Spectrophotometric Determination of Gemigliptin using Charge Transfer Complex

“…Out of the various reactions involved in spectrophotometric assay of pharmaceutical drugs, charge transfer reaction is proved to be highly sensitive as well as acceptable method, in addition to reactions involving to ion pair [9][10][11][12][13][14][15] and oxidation [16][17][18][19][20] . The complex formed between electron donor and acceptor compound yields a new absorption band with a precise l max pertaining to that complex.…”