Determination of Metronidazole in Tablet Formulations by Differential Pulse Polarography

Papas, Andrew N.; Delaney, Michael F.

doi:10.1080/00032718208069507

Cited by 18 publications

(5 citation statements)

References 3 publications

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“…Voltammetric methods have been applied to the study of the mechanism of nitroimidazoles as antimicrobial agents 20 , and its determination in pharmaceutical [21][22][23][24][25] and clinical [25][26][27] matrces. The mercury electrode was the most used, but good results were obtained using solid electrodes when studying nitrobenzene [28][29][30] and metronidazole [31][32][33] .…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Behaviour of Metronidazole at Mercury Electrodes

La-Scalea¹,

Serrano²,

Gutz³

1999

J. Braz. Chem. Soc.

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Metronidazol é o fármaco mais importante do grupo de 5-nitroimidazoles, possuindo toxicidade contra microrganismos anaeróbicos, sendo a molécula de DNA o principal alvo de sua ação biológica. O mecanismo de ação do metronidazol depende do processo de redução do grupo nitro e, em meio ácido, a redução é pH-dependente envolvendo quatro elétrons na completa redução ao derivado hidroxilamínico. Em meio aprótico a redução do metronidazole acontece em duas etapas: a primeira envolvendo um elétron para formar o radical nitro e a segunda etapa envolve mais três elétrons até a formação da hidroxilamina. Neste artigo o mecanismo de redução do metronidazol foi estudado utilizando as técnicas voltamétricas: polarografia d.c. e pulso diferencial, voltametria cíclica usando o eletrodo gota de mercúrio como eletrodo de trabalho. Metronidazole is the most important drug of the group of 5-nitroimidazoles and possesses toxicity to anaerobic microorganisms DNA being the main target for their biological action. The mechanism of biological action of metronidazole is dependent upon the nitro group reduction process. The reduction of metronidazole is pH dependent in acid medium and four electrons are involved in the complete reduction to the hydroxylamine derivative. In aprotic medium the reduction of the metronidazole occurs in two steps, the first involving one electron to form the nitro radical and the second step involving three more electrons until the formation of the hydroxylamine derivative. In this paper the mechanism of reduction of metronidazole was studied by using the voltammetric techniques: d.c. polarography, differential pulse polarography and cyclic voltammetry using the mercury drop as the working electrode.

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Section: Introductionmentioning

confidence: 99%

Voltammetric Behaviour of Metronidazole at Mercury Electrodes

La-Scalea¹,

Serrano²,

Gutz³

1999

J. Braz. Chem. Soc.

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“…To date, the majority of studies focus on its therapeutic efficacy [21], pharmaceutical technology [22,23], preclinical pharmacokinetics evaluation [24] and metabolism study in animals [25,26]. There are only a few studies concerning to the quantification of this antichagasic drug by techniques such as electrochemistry [27,28], polarography [29], spectrophotometry [30,31] and liquid chromatography (LC) associated to UV detection [32][33][34]. However, it has been well documented the limitations of some of these analytical approaches to detect interferences and overcome some serious problems related to sample complexity.…”

Section: Introductionmentioning

confidence: 99%

Development of a LC–MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine

Martínez¹,

Marson²,

Mastrantonio³

et al. 2015

Talanta

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“…Several methods have been reported for the determination of metronidazole and or tinidazole in bulk and dosage forms or in biological fluids. Such methods include; non-aqueous titration [12], voltammetric methods [13][14][15][16][17], HPLC [18,19,20,21] and flow injection analysis [22]. Some previously described spectrophotometric methods include the use of vanillin and anisaldehyde [23], p-Benzoquinone [24], Ĵ-Naphthol [25], ȕ-naphthol [26], alkaline hydrolysis [27], derivative spectrophotometry [28], extractive spectrophotometry using bromothymol blue [29], sodium 1,2-naphthaquinone-4-sulfonate after condensation reaction [30], 4-dimethylamino-cinnamaldehyde [31] and methylbenzothiazolin-2-onehydrazine (MBTH) [32] among others.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric determination of metronidazole and tinidazole via charge transfer complexation using chloranilic acid

Adegoke

Umoh

Soyinka

2010

JICS

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A new spectrophotometric method was developed for the determination of two important nitroimidazoles; metronidazole (MZ) and tinidazole (TZ). The method was based on the charge-transfer (CT) complexation reaction of reduced forms of metronidazole and tinidazole as n-electron donors and chloranilic acid (CAA) as ʌ-electron acceptor to form a purple-colored complex with a new absorption band at 520 nm which was adopted as the analytical wavelength. Molar absorptivities of 2.741 u 10 2 L M -1 cm -1 and 2.681 u 10 2 L M -1 cm -1 were obtained for MZ and TZ, respectively. Optimization of reducing agent and time of reduction revealed the superiority of metal hydrides over reducing metals. Reduction of MZ and TZ was completed at 30 °C within 10 min. Optimizations of temperature and time for the complexation reaction revealed that the reaction was completed at 30 °C within 5 min. A 60:40 mixture of 1,4-dioxane:acetonitrile was found to be the best diluting solvent for optimal detector response. The complexes were stable at room temperatures for weeks. Beer's law was observed in the concentration of 5-40 μg ml (MZ) and 4.8-79.2 μg ml -1 (TZ) with low limits of detection of 1.88 and 0.74 μg ml -1 , respectively. Overall recoveries of MZ and TZ from quality control samples were 103.19 ± 2.05 (%RSD = 1.99, n = 12) and 101.63 ± 1.41 (%RSD = 1.39) over three days. There was no interference from commonly utilized tablet excipients. No significant difference existed between the results of the new method and the BP titrimetric procedures (p > 0.05). The new CT procedure described in this paper is simple, fast, convenient, accurate and precise and has the novelty of carrying out the reactions at room temperature compared to previously described procedures.The new method could be adopted as an alternative procedure for the quality assessment of MZ and TZ in bulk and dosage forms.

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Determination of Metronidazole in Tablet Formulations by Differential Pulse Polarography

Cited by 18 publications

References 3 publications

Voltammetric Behaviour of Metronidazole at Mercury Electrodes

Voltammetric Behaviour of Metronidazole at Mercury Electrodes

Development of a LC–MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine

Spectrophotometric determination of metronidazole and tinidazole via charge transfer complexation using chloranilic acid

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