Determination of methylparaben, propylparaben, triamcinolone acetonide and its degradation product in a topical cream by RP-HPLC

Abstract

A novel reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the determination of active component triamcinolone acetonide, its degradation product triamcinolone (occurring in formulation after long-term stability tests) and two preservatives presented in the cream-methylparaben and propylparaben, using hydrocortisone as an internal standard. The chromatographic separation was performed on a Supelco Discovery C18 column; the mobile phase for separation of all compounds consists of a mixture of acetonitrile and water (40:60 v/v). The analysis time was less than 9 min, at a flow rate of 0.6 mL min(-1) and detection at 240 nm. The method was found to be applicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product topical cream Triamcinolon cream 0.1%.

Application of triamcinolone acetonide paste to the endotracheal tube reduces postoperative sore throat: a randomized controlled trial

“…Moreover, triamcinolone acetonide contains two preservatives, methylparaben and propylparaben, which are effective antibacterial and antifungal agents commonly used as preservatives in foods, beverages, and pharmaceuticals. 17 Chlorehexidine gluconate jelly is used frequently for lubrication. Thus, we used chlorhexidine gluconate jelly for comparison with triamcinolone acetonide paste.…”

Characterisation of systemic and ocular drug level of triamcinolone acetonide following a single sub-Tenon injection

“…The mobile phase was composed of a mixture of acetonitrile and 0.1% formic acid (50:50, volume/volume). Acetonitrile has been used as a mobile phase for separation of TA using liquid chromatography (HPLC) or LC-tandem MS in recent years18–20 and the method has been validated 18 19. The flow rate was set at 0.3 ml/min.…”

Simultaneous Determination of NSAID and Antimicrobial Preservatives Using Validated RP-HPLC Method: An Application in Pharmaceutical and Clinical Laboratories

“…[21][22][23][24][25][26][27][28][29][30][31][32], gradient HPLC [33], solid phase extraction HPLC [34], HPLC-MS [35].…”