Determination of methylmercury in fish tissues by isotope dilution SPME-GC-ICP-MS

Abstract

Access and use of this website and the material on it are subject to the Terms and Conditions set forth at Determination of methylmercury in fish tissues by isotope dilution SPME-GC-ICP-MS Yang, L.; Mester, Z.; Sturgeon, R.http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=fr L'accès à ce site Web et l'utilisation de son contenu sont assujettis aux conditions présentées dans le site LISEZ CES CONDITIONS ATTENTIVEMENT AVANT D'UTILISER CE SITE WEB. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?action=rtdoc&an=8899014&lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?action=rtdoc&an=8899014&lang=fr READ THESE TERMS AND CONDITIONS CAREFULLY BEFORE USING THIS WEBSITE.http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://doi.org/10.1039/b301299aJournal of Analytical Atomic Spectrometry, 18, 5, pp. 431-436, 2003 Determination of methylmercury in fish tissues by isotope dilution SPME-GC-ICP-MS{ A method is described for the accurate and precise determination of monomethylmercury (MMHg) by species specific isotope dilution (ID) calibration using solid phase microextraction (SPME) in combination with gas chromatographic (GC) separation and inductively coupled plasma mass spectrometric (ICP-MS) detection. Samples were digested with methanolic potassium hydroxide, derivatized in aqueous solution with sodium tetrapropylborate and headspace sampled with a polydimethylsiloxane coated SPME fused silica fiber. The analyte was then directly transferred from the fiber to the head of the GC column for desorption by insertion of the fiber through the heated injection port. Reverse spike ID analysis was performed to determine the accurate concentration of an in-house synthesized 198 Hg-enriched monomethylmercury (MM 198 Hg) spike [candidate Certified Reference Material (CRM) EOM-1] using two natural abundance MMHg standards. Concentrations of 0.719 ¡ 0.015 and 4.484 ¡ 0.029 mgg 21 (one standard de...

Blank correction considerations for isotope dilution and reverse isotope dilution calibration: Determination of methylmercury in fish tissue

“…No significant difference in measured MeHg concentrations was obtained when either isotope pair 202 Hg/ 198 Hg or 200 Hg/ 198 Hg was used for the calculations based on eqn. (9). The 202 Hg/ 198 Hg isotope pair was thus selected for the final quantitation of MeHg because of the higher abundance of this isotope (better sensitivity).…”

“…As SPME extraction efficiency can be influenced by a number of factors, including extraction temperature, extraction time, pH of the solution and the concentration of derivatization reagent, optimized parameters were taken from an earlier SPME GC-ICP-MS study. 9 Optimum sample pH was found to be in the range 4 to 7. A pH 5 obtained with a 0.5 M NH 4 OAc or 1 M NaOAc buffer was used.…”

Species-specific isotope dilution-GC-ICP-MS for accurate and precise measurement of methylmercury in water, sediments and biological tissues

“…To tackle these above mentioned problems of losses and chemical transformation of MeHg in sample cleanup and derivatization, the isotope dilution (ID) technique is a good alternative method 14,15,22 since quantitative recoveries are not necessary, once the enriched isotope is fully equilibrated with the natural isotope. Isotope dilution mass spectrometry has been considered to be an absolute method, which can play an important role as the reference method for MeHg analysis in different environmental matrices.…”

Species specific isotope dilution for the accurate and SI traceable determination of arsenobetaine and methylmercury in cuttlefish and prawn

“…Sample preparation for the determination of MeHg followed previous reported procedures [11,33,34]. In brief, 250 mg subsamples were spiked with an appropriate mass of the enriched Me 198 Hg spike solution (0.12 mgg À1 ).…”