Determination of methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteria in blood by capillary zone electrophoresis

Horká, Marie; Tesařová, Marie; Karásek, Pavel; Růžička, Filip; Holá, Veronika; Sittová, Martina; Roth, Michal

doi:10.1016/j.aca.2015.02.001

Cited by 23 publications

(28 citation statements)

References 50 publications

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“…Throughout the pH range, the EOF in the capillaries etched with SCW was higher than that in the original smooth, single diameter, FS capillaries, curves 1 versus 4. A significant decrease in EOF occurred after the modification of the original smooth FS capillary (curve 2) and the etched FS capillary (curve 5) with GOTMS such as in . However, EOF was higher (curve 5) in comparison to the SCW‐treated capillary with the surface roughness gradient – coarse‐to‐fine orientation (Fig.…”

Section: Resultsmentioning

confidence: 94%

Fused silica capillaries with two segments of different internal diameters and inner surface roughnesses prepared by etching with supercritical water and used for volume coupling electrophoresis

et al. 2017

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In this work, single-piece fused silica capillaries with two different internal diameter segments featuring different inner surface roughness were prepared by new etching technology with supercritical water and used for volume coupling electrophoresis. The concept of separation and online pre-concentration of analytes in high conductivity matrix is based on the online large-volume sample pre-concentration by the combination of transient isotachophoretic stacking and sweeping of charged proteins in micellar electrokinetic chromatography using non-ionogenic surfactant. The modified surface roughness step helped to the significant narrowing of the zones of examined analytes. The sweeping and separating steps were accomplished simultaneously by the use of phosphate buffer (pH 7) containing ethanol, non-ionogenic surfactant Brij 35, and polyethylene glycol (PEG 10000) after sample injection. Sample solution of a large volume (maximum 3.7 μL) dissolved in physiological saline solution was injected into the wider end of capillary with inlet inner diameter from 150, 185 or 218 μm. The calibration plots were linear (R ∼ 0.9993) over a 0.060-1 μg/mL range for the proteins used, albumin and cytochrome c. The peak area RSDs from at least 20 independent measuremens were below 3.2%. This online pre-concentration technique produced a more than 196-fold increase in sensitivity, and it can be applied for detection of, e.g. the presence of albumin in urine (0.060 μg/mL).

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Section: Resultsmentioning

confidence: 94%

Fused silica capillaries with two segments of different internal diameters and inner surface roughnesses prepared by etching with supercritical water and used for volume coupling electrophoresis

et al. 2017

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“…4. Capillary electrophoresis has already been useful in the identification of pathogens [5][6][7], yeast cells [8] or various types of viruses [9,10]. A CE approach has also been applied for the direct detect of the Hela cells apoptosis [40].…”

Section: Resultsmentioning

confidence: 99%

“…This technique has been found to provide many advantages such as high separation efficiency, short analysis time or the possibility of direct analysis of biological samples [1][2][3][4]. There is a few examples of the CE use for the determination of bacterial pathogens [5][6][7], yeast cells [8] or various types of human and plant viruses [9,10]. Although this analytical method provides many solutions in the field of the microorganisms identification, it is important to remember about its limitations and drawbacks such as uncontrolled aggregation (clumping) of bacterial cells and their adhesion to the capillary surface [5,11].…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions

2018

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The developed capillary electrophoresis (CE) method was applied to evaluate the aggregation activity of divalent metal ions (zinc) on bacterial cells. In our study, the influence of the Lactococcus lactis surface modification by zinc ions at different concentration (1, 3 and 10 mM, respectively) was determined. Electrophoretic results in pseudo isotachophoretic mode indicated that using a Zn aq 2+ caused the uncontrolled aggregation of bacterial cells and, as a result, new signals of microbial agglomerates were observed in the electropherogram. Moreover, the observed process is strongly related with the concentration of the used Zn aq 2+ solution-the number of bacterial agglomerates increased with an increasing concentration of modifier. The phenomenon of L. lactis uncontrolled clumping was also evaluated by application of size distribution and spectrometric in infrared range measurements (FT-IR) measurements. The obtained data indicated that the main groups involved in the bacterial cells aggregation process are deprotonated carboxyl and amid groups derived from microbial surface proteins. As a consequence, the proposed mechanism of the L. lactis uncontrolled clumping process is presented. In addition, data from fluorescence microscopy pointed out damage cells analyzed direct after electroanalysis. Therefore, CE may be a potential method for evaluating the aggregation activity of different types of bivalent metal ions against microbial cells.

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“…The possible advantages of the FS capillary roughness have been demonstrated by the separation of different Staphylococcus aureus strains which are usually difficult to carry out. The capillaries with a roughness of 2 μm were prepared by etching with supercritical water (SCW) and were used for the CE separation of these cells with a diameter of ∼1 μm . To prevent an interaction between the analytes and the inner wall of the capillary together with simultaneous control of the EOF, it has been necessary to silanize the inner surface of the capillary and to add suitable additives in the BGEs of their appropriate pH .…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis in a fused-silica capillary with surface roughness gradient

et al. 2016

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The electro-osmotic flow, a significant factor in capillary electrophoretic separations, is very sensitive to small changes in structure and surface roughness of the inner surface of fused silica capillary. Besides a number of negative effects, the electro-osmotic flow can also have a positive effect on the separation. An example could be fused silica capillaries with homogenous surface roughness along their entire separation length as produced by etching with supercritical water. Different strains of methicillin-resistant and methicillin-susceptible Staphylococcus aureus were separated on that type of capillaries. In the present study, fused-silica capillaries with a gradient of surface roughness were prepared and their basic behavior was studied in capillary zone electrophoresis with UV-visible detection. First the influence of the electro-osmotic flow on the peak shape of a marker of electro-osmotic flow, thiourea, has been discussed. An antifungal agent, hydrophobic amphotericin B, and a protein marker, albumin, have been used as model analytes. A significant narrowing of the detected zones of the examined analytes was achieved in supercritical-water-treated capillaries as compared to the electrophoretic separation in smooth capillaries. Minimum detectable amounts of 5 ng/mL amphotericin B and 5 μg/mL albumin were reached with this method.

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Determination of methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteria in blood by capillary zone electrophoresis

Cited by 23 publications

References 50 publications

Fused silica capillaries with two segments of different internal diameters and inner surface roughnesses prepared by etching with supercritical water and used for volume coupling electrophoresis

Fused silica capillaries with two segments of different internal diameters and inner surface roughnesses prepared by etching with supercritical water and used for volume coupling electrophoresis

Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions

Capillary electrophoresis in a fused-silica capillary with surface roughness gradient

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