Determination of Metal Ions by High Performance Liquid Chromatography

Abstract

The mixture of metal ions [Bi(III), Fe(III), Fe(II), Cu(II), Zn(II), Ni(II), Co(II), Pb(II), Cd(II), Mn(II)] were separated in the bonded‐phase strong cation exchange column (Vydac‐401 SA) and monitored at 540 nm after a postcolumn reaction with 4‐(2‐pyridylazo)‐resorcinol (PAR). Citrate, tartrate, lactate and α‐hydroxyisobutyrate buffer were used as eluent and it has been found that the elution order of some metal ions were changed with different eluents. The detection limits and the calibration curves of metal ions were also studied.

Separation of metal ions using a polymeric reversed-phase column and a Methylthymol Blue containing mobile phase

“…For Zn 2+ and Pb 2+ the sensitivity achieved using MTB is of the same order as that obtained using ion exchange chromatography with post-column reaction with PAR, which was quoted as being 0.10-0.75 mm for Zn 2+ and 0.58-0.72 mm for Pb 2+ (50 ml injection). [9][10] The reproducibility (%RSD) of the method was investigated with the repeat injection (n = 7) of a 0.1 mm Zn 2+ standard and was found to be 3.9% for peak height and 4.8% for peak area.…”

Ion-chromatographic separation of metal cations in the presence of a-hydroxyisobutyric acid

“…Applications of HIBA to the separations of other polyvalent metal cations such as actinoids [16], In>, Al>, Ga>, Zr> [12], Th> and Pu> [17], Th> and UO> [18,19] are less common. Only exceptionally has HIBA been employed for the chromatographic separations of divalent metal cations [8,12,20,21]. HIBA was also successfully used in other separation techniques such as capillary isotachophoresis (ITP) [22][23][24] and capillary electrophoresis (CE) [25,26] -again for the separations of lanthanides in the first place.…”

Study on Determination of Six Transition Metal Ions in Biological Samples by SPE and RP‐HPLC

“…The simultaneous determination of metal ions as they chelate with organic reagents by RP-HPLC technique has been receiving increasing attention and has been widely applied to inorganic analysis in recent years. [1][2][3][4] Many azo dyes such as pyridylazo reagents, thiazolylazo reagents, benzothiazolylazo reagents and the like have been used as chelating reagents for the determination of metal ions by RP-HPLC. [5][6][7][8][9][10][11] But the 2-quinolinylazo derivatives have received little attentions.…”