Determination of metal content in superoxide dismutase enzymes by capillary electrophoresis†

Kazarjan, Jana; Vaher, Merike; Hunter, Thérèse; Kulp, Maria; Hunter, Gary J.; Bonetta, Rosalin; Farrugia, Diane; Kaljurand, Mihkel

doi:10.1002/jssc.201400925

Cited by 8 publications

(9 citation statements)

References 21 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The co‐factor of enzymatic antioxidants is a metallic core that participates in the redox reactions. Methods such as capillary electrophoresis have been developed to characterize the enzymatic activity of antioxidants through the measurement of metal content in the enzyme . Nitroblue tetrazolium (NBT) reduction is used to assess O 2 ●− production by xanthine oxidase .…”

Section: Measuring Cellular Oxidative Stressmentioning

confidence: 99%

Oxidative stress by inorganic nanoparticles

Tee

Ong

Bay

et al. 2015

WIREs Nanomed Nanobiotechnol

View full text Add to dashboard Cite

Metallic and metallic oxide nanoparticles (NPs) have been increasingly used for various bio-applications owing to their unique physiochemical properties in terms of conductivity, optical sensitivity, and reactivity. With the extensive usage of NPs, increased human exposure may cause oxidative stress and lead to undesirable health consequences. To date, various endogenous and exogenous sources of oxidants contributing to oxidative stress have been widely reported. Oxidative stress is generally defined as an imbalance between the production of oxidants and the activity of antioxidants, but it is often misrepresented as a single type of cellular stress. At the biological level, NPs can initiate oxidative stress directly or indirectly through various mechanisms, leading to profound effects ranging from the molecular to the disease level. Such effects of oxidative stress have been implicated owing to their small size and high biopersistence. On the other hand, cellular antioxidants help to counteract oxidative stress and protect the cells from further damage. While oxidative stress is commonly known to exert negative biological effects, measured and intentional use of NPs to induce oxidative stress may provide desirable effects to either stimulate cell growth or promote cell death. Hence, NP-induced oxidative stress can be viewed from a wide paradigm. Because oxidative stress is comprised of a wide array of factors, it is also important to use appropriate assays and methods to detect different pro-oxidant and antioxidant species at molecular and disease levels. WIREs Nanomed Nanobiotechnol 2016, 8:414-438. doi: 10.1002/wnan.1374 For further resources related to this article, please visit the WIREs website.

show abstract

Section: Measuring Cellular Oxidative Stressmentioning

confidence: 99%

Oxidative stress by inorganic nanoparticles

Tee

Ong

Bay

et al. 2015

WIREs Nanomed Nanobiotechnol

View full text Add to dashboard Cite

show abstract

“…Catalase activity was determined by the decomposition of hydrogen peroxide [15][16]. Activity superoxide dismutase (SOD) determined in the system nitro-blue tetrazoliumphenazinemethosulfate-nicotinamidedinucleotide (NBT-PMS-NADN).…”

Section: Methodsmentioning

confidence: 99%

Effects of Bacillus subtilis and Bacillus licheniformis on catfish in industrial aquaculture

et al. 2020

View full text Add to dashboard Cite

When growing fish in high-tech breeding systems, it is exposed to many stress factors. To reduce stress, we used spore forms of microorganisms — Bacillus subtilis and Bacillus licheniformis, possessing probiotic properties. Two groups were formed, a control group and an experimental group that received probiotic microorganisms with feed. The aim of the study was to evaluate the effectiveness of the use of microorganisms — Bacillus subtilis and Bacillus licheniformis to reduce stress in fishes. For this indicator points were selected — stress hormone cortisol and antioxidant defense enzymes. The research results showed that stress level assessed by cortisol in fishes fed with probiotic microorganisms is 2.8 times lower than in the control group. The use of micronucleus test to assess cytogenetic damage showed that against the probiotic microbiota background, the number of cells with fragmented nuclei decreases by 5.7 times. Antioxidant defense enzyme activity when using Bacillus subtilis and Bacillus licheniformis increased, indicating activation of the defense system against oxidative stress. The obtained results demonstrated the high efficiency of the use of spore forms Bacillus subtilis and Bacillus licheniformis when growing catfish in high-tech fish farming systems. The Russian Foundation for Basic Research has supported our study with the grant No. 18-016-00127.

show abstract

“…Анализ ферментативной активности (ед./ мг белка) осуществляли на двулучевом спектрофотометре UV-1800 (Shimadzu, Япония), длина волны λ=480 нм и λ=347 нм, при комнатной температуре, учитывая тот факт, что исследовали пойкилотермных организмов в физиологически адекватных условиях. Активность каталазы определяли по реакции разложения перекиси водорода [9][10][11]. Активность супероксиддисмутазы (СОД) определялась спектрофотометрическим методом в системе нитро-синий тетразолиевый-феназинметасульфат-никотинамиддинуклеотид (НСТ-ФМС-НАДН).…”

Section: объекты и методы исследованийunclassified

Stimulation effect of antioxidant system of fish against use of probiotics of spore term

Спирина¹,

Romanova²,

Romanov³

et al. 2020

Vestnik of Ulyanovsk SAA

View full text Add to dashboard Cite

Ключевые слова: аквакультура, африканский клариевый сом, антиоксидантная защита, эритроциты, супероксиддисмутаза, каталаза.В организме рыб функционирует антиоксидантная система, состоящая из ферментативного и неферментативного звеньев, которые обеспечивают связывание радикалов, предупреждают образование или разрушают перекиси. Целью нашей работы являлось исследование влияния пробиотика споротермин на процессы свободнорадикального окисления в организме африканских клариевых сомов. Одними из важнейших элементов антиоксидантной защиты организма рыб являются супероксиддисмутаза (СОД) и каталаза. СОД -это группа металлоферментов, обеспечивающих ускорение реакции дисмутации супероксидных анион-радикалов, за счет последовательного восстановления и окисления, в активном центре фермента ионов металлов с переменной валентностью. Каталаза -это геминовый фермент, содержащийся в пероксисомах, который обеспечивает разложение перекиси водорода на молекулярный кислород и воду. Главная роль каталазы в клетках у рыб -это разрушение перекиси водорода, которая образуется в клетках вследствие действия оксидаз, а также обеспечение защиты от перекиси водорода, способной разрушить клеточные структуры. В своих исследованиях мы использовали споротермин, который относится к числу пробиотиков последнего поколения. Результаты исследований показали, что у клариевых сомов, выращенных с использованием пробиотика споротермин, наблюдается увеличение активности СОД и каталазы в эритроцитах и в сыворотке крови. При исследовании антиоксидантной системы клариевых сомов было установлено, что использование пробиотика споротермин приводит к повышению активности ферментов антиоксидантной системы, тем самым стимулируя антиоксидантную защиту организма рыб.

show abstract

Determination of metal content in superoxide dismutase enzymes by capillary electrophoresis†

Cited by 8 publications

References 21 publications

Oxidative stress by inorganic nanoparticles

Oxidative stress by inorganic nanoparticles

Effects of Bacillus subtilis and Bacillus licheniformis on catfish in industrial aquaculture

Stimulation effect of antioxidant system of fish against use of probiotics of spore term

Contact Info

Product

Resources

About