Determination of Metal Associated with Proteins of Wheat Seed Samples After Sequential Extraction Procedure

Bittencourt, Leila Moreira; Lana, Diogo A. P. D.; Pimenta, Adriano Monteiro de Castro; Santos, Agenor Valadares; Gonçalves, Ana Paula; Augusti, Rodinei; Costa, Letícia M.

doi:10.5935/0103-5053.20130292

Cited by 5 publications

(3 citation statements)

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“…3 ). Tris/HCl is a common buffer used to extract metal-bound species from different genotypes of wheat [18 , 24] , probably for its lysis properties which can explain its high extraction ability. Ammonium nitrate is used as a fertilizer in agriculture to provide a source of nitrogen which stimulates the plant growth, and it was chosen because it was considered a suitable solvent to extract water-soluble plant available trace elements from soil [21 , 22] and toxic elements from contaminated grassland soils to assess metal mobility in soil [19] .…”

Section: Extractionmentioning

confidence: 99%

Development of a method for the detection of zinc in Brassica oleracea using solid phase extraction and size-exclusion chromatography inductively coupled plasma mass spectrometry (SEC-ICP-MS)

Dell'Aquila

2021

MethodsX

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The aim of this work is the development of a suitable method to extract and detect zinc-bound compounds from cabbage, broccoli and kale (family Brassicaceae, species oleracea) using solid phase extraction (SPE) and size-exclusion chromatography inductively coupled plasma mass spectrometry (SEC-ICP-MS). Tris [2-Amino-2-(hydroxymethyl)-1,3-propanediol]/hydrochloric acid (Tris/HCl) or ammonium nitrate were used as extractants added to the freeze-dried samples, which were then sonicated and centrifuged. An enzymatic mixture was added to the extracts and then incubated for 5- and 18 h prior to analysis by SEC-ICP-MS. Results showed a good coefficient of variation (CV) of the elution time (0.06-0.9%), concentration (4.7–16.9%) and molecular size (0.4–5.4%). The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.9 μg L−1 and 2.8 μg L−1, respectively. The proposed method is reliable and robust and can be applied to samples with difficult matrices like vegetables and soil. Good precision, stability and reproducibility. Easy to execute and suitable for analysis of vegetables and other samples with complex matrices, eg. soil. This method contributed to good maintenance of the instrument and to minimal cleaning time.

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Section: Extractionmentioning

confidence: 99%

Development of a method for the detection of zinc in Brassica oleracea using solid phase extraction and size-exclusion chromatography inductively coupled plasma mass spectrometry (SEC-ICP-MS)

Dell'Aquila

2021

MethodsX

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“…Since neutrophils are the predominant cell type in HSF with RA, most proteins are neutrophil-related (i.e. azurocidin, defensin 2, neutrophil gelatinase associated lipocalin or the leukocyte elastase inhibitor) [35][36][37].…”

Section: Electrophoretic Separation Of Hsf Proteins and Characterizationmentioning

confidence: 99%

ICPMS analysis of proteins separated by Native-PAGE: Evaluation of metaloprotein profiles in human synovial fluid with acute and chronic arthritis

Moyano

Mariño-Repizo

Tamashiro³

et al. 2016

Journal of Trace Elements in Medicine and Biology

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“…Only a limited number of papers have been published on the elemental mapping of seeds using LA-ICP-MS (Basnet, Amarasiriwardena et al 2014, Bittencourt, Lana et al 2012, Cakmak, Kalayci et al 2010, Han, Quinet et al 2013, Wu, Andersch et al 2013a, Wu, Andersch et al 2013b, with even less of full images (Wu, Andersch et al 2013b, Basnet, Amarasiriwardena et al 2014. Therefore, the work presented in this chapter further demonstrates the potential application for LA-ICP-MS in agricultural studies.…”

Section: Quantitative Laser Ablation Inductively Coupled Plasma Mass mentioning

confidence: 78%

Investigation of nutrient solutions for the hydroponic growth of plants

Seaman¹

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I would first like to thank the EPRSC for funding my PhD and Prof Neil Bricklebank for being an understanding and patient supervisor. I would like to give special thanks to my and dear friend Ian Stansfield, for spending many hours' proof reading my thesis and correcting my work. I would like to thank Michael Cox of the Technical staff at Sheffield Hallam University, who spent many hours helping me with many instruments of various ages and capabilities. Without him I would not have half the data I do. I would also like to thank Gareth Hopcroft for his help during the Afterlife project. Thank you to Bence Paul and Dany Savard of Iolite 3.32 who gave me so much support with the data processing of the LA-ICP-MS. Bob Burton and Deeba Zahoor of MERI, Sheffield Hallam university, for performing analysis with XRD, XRF and Raman Spectroscopy. Also, I would like to thank Dr Antonio Feteira of MERI for allowing me to use their ball mill. I would like to thank Bryn Flinders of the FOM Institute AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands for performing the Meta-SIMS imaging of the radish. I would like to thank all my friends and family for believing in me and keeping me focused during some very difficult times, particularly, Ian Dickinson Emma Pearson and Lucy Sweeny. I would like to thank my husband David Seaman and son Baden Fixter-Seaman for supporting me through this difficult journey and listening to me moan about everything.

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Determination of Metal Associated with Proteins of Wheat Seed Samples After Sequential Extraction Procedure

Cited by 5 publications

References 0 publications

Development of a method for the detection of zinc in Brassica oleracea using solid phase extraction and size-exclusion chromatography inductively coupled plasma mass spectrometry (SEC-ICP-MS)

Development of a method for the detection of zinc in Brassica oleracea using solid phase extraction and size-exclusion chromatography inductively coupled plasma mass spectrometry (SEC-ICP-MS)

ICPMS analysis of proteins separated by Native-PAGE: Evaluation of metaloprotein profiles in human synovial fluid with acute and chronic arthritis

Investigation of nutrient solutions for the hydroponic growth of plants

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